breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 33 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG46.9% 283.2 / 90.0 228intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC39.8% 306.3 / 79.2 207G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306341,3770TG32.6% 173.6 / 23.4 99G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123063,527,3680AC32.6% 511.2 / 63.6 242L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306935,0540GT32.1% 165.9 / 45.8 84intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123062,680,3450AC30.5% 316.8 / 46.3 179T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit
*AP012306800,6750AC29.3% 533.9 / 40.3 240T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123063,639,0400TG28.9% 147.4 / 12.6 76noncoding (54/76 nt)gltVtRNA‑Glu
*AP012306644,4170GA27.4% 1011.3 / inf 569intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,938,8870CT27.1% 504.7 / 142.3 247S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123061,128,4120TG26.9% 612.8 / 56.9 258V29G (GTG→GGG) yciOconserved protein
*AP012306202,4170TG26.6% 528.1 / 24.2 232V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP012306257,1370TG26.4% 368.0 / 20.1 178L496V (TTG→GTG) betTcholine transporter of high affinity
*AP0123062,212,7530AC25.2% 286.8 / 33.6 119Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123061,226,5570AG24.8% 341.8 / 55.5 150G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123062,560,2430TG24.6% 421.2 / 22.3 171N419T (AAC→ACC) pepPproline aminopeptidase P II
*AP0123061,421,1470TG24.5% 420.7 / 10.2 207T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
*AP0123062,558,0710AC24.1% 517.7 / 27.8 212V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123061,066,3230AC23.3% 594.0 / 13.2 215intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
*AP0123063,282,0760TG23.1% 534.7 / 18.7 238intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide

Marginal new junction evidence (lowest skew 10 of 104 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 215364NA (NA)14 (0.050) 12/288 8.1 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 1092334293 (0.980)10 (0.040) 9/258 8.5 3.5% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 297 (1.150)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 42209248 (0.830)7 (0.030) 7/270 9.2 3.0% coding (139/531 nt) kefF flavoprotein subunit for the KefC potassium efflux system
?AP012306 = 42195 231 (0.860)coding (125/531 nt) kefF flavoprotein subunit for the KefC potassium efflux system
* ? AP012306 = 2490688287 (0.960)7 (0.020) 7/290 9.4 2.4% coding (483/693 nt) ygeA predicted racemase
?AP012306 = 2490701 287 (0.990)coding (470/693 nt) ygeA predicted racemase
* ? AP012306 = 1092512356 (1.200)14 (0.050) 6/258 9.4 4.4% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 298 (1.160)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 3597644 =246 (0.830)7 (0.030) 6/274 9.6 3.0% noncoding (60/76 nt) gltT tRNA‑Glu
?AP012306 3639058 = NA (NA)noncoding (72/76 nt) gltV tRNA‑Glu
* ? AP012306 = 3240654429 (1.440)6 (0.020) 6/274 9.6 1.5% coding (234/237 nt) rpmB 50S ribosomal subunit protein L28
?AP012306 = 3240642 388 (1.420)intergenic (‑12/+9) rpmG/rpmB 50S ribosomal subunit protein L33/50S ribosomal subunit protein L28
* ? AP012306 = 2275071224 (0.750)45 (0.170)
+GTATCAGTCTGCTTCGCAAG
5/258 9.7 15.9% noncoding (1/76 nt) gltW tRNA‑Glu
?AP012306 = 3597478 326 (1.100)intergenic (+65/‑107) rrsB/gltT 16S ribosomal RNA (rrnB)/tRNA‑Glu
* ? AP012306 = 3805843360 (1.210)6 (0.020) 6/290 9.8 1.7% intergenic (+42/‑134) ecnB/sugE entericidin B membrane lipoprotein/multidrug efflux system protein
?AP012306 = 3805868 338 (1.170)intergenic (+67/‑109) ecnB/sugE entericidin B membrane lipoprotein/multidrug efflux system protein
* ? AP012306 = 3017771281 (0.940)6 (0.020) 6/290 9.8 2.2% coding (737/2448 nt) glgP glycogen phosphorylase
?AP012306 = 3017781 265 (0.920)coding (727/2448 nt) glgP glycogen phosphorylase