breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 26 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG47.5% 322.5 / 88.9 257intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC38.2% 348.5 / 59.3 206G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306341,3770TG35.1% 144.1 / 17.6 94G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP012306644,4170GA32.9% 757.0 / inf 568intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,527,3680AC32.4% 441.3 / 56.1 222L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123062,758,9090TG30.1% 523.3 / 29.0 236G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP012306800,6750AC28.3% 535.8 / 45.2 244T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123062,680,3450AC28.2% 362.9 / 36.0 170T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit
*AP0123062,558,0710AC28.1% 491.3 / 32.9 224V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123061,854,8510AC25.9% 709.6 / 77.3 293T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP012306524,2890TG24.2% 328.3 / 13.6 133N4T (AAC→ACC) citDcitrate lyase, acyl carrier (gamma) subunit
*AP0123063,938,8870CT24.1% 639.9 / 129.8 278S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP012306202,4170TG23.8% 604.6 / 28.1 245V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP012306472,8680TG23.7% 568.2 / 16.2 220D435A (GAC→GCC) cusSsensory histidine kinase in two‑component regulatory system with CusR, senses copper ions
*AP0123063,282,0760TG23.1% 571.3 / 17.4 256intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP0123062,714,5550TG22.6% 716.5 / 16.6 293T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP0123063,116,3540TG22.3% 532.7 / 10.8 221T307P (ACC→CCC) yhjGpredicted outer membrane biogenesis protein
*AP0123061,226,5570AG21.9% 457.9 / 66.4 184G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123061,128,4120TG21.8% 683.8 / 16.6 258V29G (GTG→GGG) yciOconserved protein
*AP0123063,324,4330TA21.5% 515.2 / 73.6 205intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein

Marginal new junction evidence (lowest skew 10 of 71 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 1092512352 (1.090)15 (0.050) 12/258 9.9 4.8% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 292 (1.040)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 997497299 (0.920)10 (0.030) 9/268 11.0 3.5% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 277 (0.950)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 644106 =509 (1.570)14 (0.040)
+CCCGG
9/288 11.3 3.5% noncoding (5/76 nt) lysZ tRNA‑Lys
?AP012306 = 2073541 301 (0.930)intergenic (+1/‑46) valX/valY tRNA‑Val/tRNA‑Val
* ? AP012306 644106 =509 (1.570)14 (0.050)
+CCCGGG
9/286 11.3 3.2% noncoding (5/76 nt) lysZ tRNA‑Lys
?AP012306 = 2073420 383 (1.180)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 = 1092334328 (1.010)8 (0.030) 7/258 11.5 2.7% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 292 (1.040)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 2410909 =473 (1.460)7 (0.020) 7/286 12.0 1.5% intergenic (‑101/‑218) ygcW/yqcE predicted deoxygluconate dehydrogenase/predicted transporter
?AP012306 2410933 = 458 (1.480)intergenic (‑125/‑194) ygcW/yqcE predicted deoxygluconate dehydrogenase/predicted transporter
* ? AP012306 1359429 =319 (0.990)7 (0.020) 7/296 12.1 2.1% coding (58/1533 nt) pntA pyridine nucleotide transhydrogenase, alpha subunit
?AP012306 1359444 = 330 (1.030)coding (43/1533 nt) pntA pyridine nucleotide transhydrogenase, alpha subunit
* ? AP012306 = 966009385 (1.190)6 (0.020) 6/292 12.4 1.6% coding (228/561 nt) yceB predicted lipoprotein
?AP012306 = 966026 375 (1.180)coding (211/561 nt) yceB predicted lipoprotein
* ? AP012306 = 2275012298 (0.920)5 (0.020) 5/274 12.6 1.8% noncoding (60/76 nt) gltW tRNA‑Glu
?AP012306 3597656 = 282 (0.950)noncoding (72/76 nt) gltT tRNA‑Glu
* ? AP012306 186236 =363 (1.120)6 (0.020) 5/290 12.8 1.7% coding (830/1353 nt) yaeL zinc metallopeptidase
?AP012306 186261 = 351 (1.120)coding (855/1353 nt) yaeL zinc metallopeptidase