breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG47.2% 345.9 / 109.0 268intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123063,020,6700TG33.1% 298.8 / 10.3 170K195N (AAA→AACglgCglucose‑1‑phosphate adenylyltransferase
*AP012306644,4170GA31.4% 711.8 / inf 481intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,527,3680AC29.1% 558.6 / 51.7 237L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123061,226,5570AG28.6% 386.9 / 87.5 192G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123063,938,8870CT28.6% 412.2 / 115.4 203S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123061,181,3690AC28.5% 351.3 / 31.0 144K103N (AAA→AACycjSpredicted oxidoreductase, NADH‑binding
*AP0123061,854,8510AC28.0% 592.6 / 61.0 255T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123063,182,8610AC26.9% 305.1 / 21.5 130L198F (TTA→TTCyiaNpredicted transporter
*AP012306341,3770TG26.2% 294.5 / 14.3 150G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123061,944,2880AC25.3% 581.5 / 35.2 242G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,590,3670AC24.7% 652.6 / 36.5 255T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP0123062,212,7530AC24.6% 345.1 / 38.0 142Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123062,714,5550TG24.5% 685.9 / 32.8 287T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP0123063,324,4330TA24.4% 535.3 / 92.8 234intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
*AP0123063,282,0760TG24.1% 600.6 / 10.0 253intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP012306935,0540GT24.0% 346.2 / 59.2 121intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123061,074,6190TC23.0% 365.4 / 105.1 187S29G (AGC→GGC) ldrAtoxic polypeptide, small
*AP012306202,4170TG22.5% 694.9 / 27.5 284V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123061,128,4120TG22.3% 675.9 / 32.2 265V29G (GTG→GGG) yciOconserved protein

Marginal new junction evidence (lowest skew 10 of 140 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 215364NA (NA)16 (0.050) 16/288 19.1 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 1092512382 (1.220)14 (0.050) 12/258 19.7 4.3% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 300 (1.110)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 2424031 =322 (1.030)9 (0.030) 9/270 21.6 2.9% coding (156/2235 nt) relA (p)ppGpp synthetase I/GTP pyrophosphokinase
?AP012306 2424045 = 303 (1.070)coding (142/2235 nt) relA (p)ppGpp synthetase I/GTP pyrophosphokinase
* ? AP012306 = 1092334318 (1.010)8 (0.030) 7/258 22.4 2.7% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 299 (1.100)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 152555308 (0.980)10 (0.030) 9/292 22.4 3.2% coding (3/480 nt)
coding (1397/1398 nt)
folK
pcnB
2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase
poly(A) polymerase I
?AP012306 = 152570 306 (0.990)coding (1382/1398 nt) pcnB poly(A) polymerase I
* ? AP012306 = 3959350319 (1.020)9 (0.030) 8/286 22.8 2.8% intergenic (‑18/‑88) ytjB/serB conserved protein/3‑phosphoserine phosphatase
?AP012306 = 3959401 325 (1.080)intergenic (‑69/‑37) ytjB/serB conserved protein/3‑phosphoserine phosphatase
* ? AP012306 179936 =448 (1.430)8 (0.030) 8/288 22.8 1.8% coding (219/852 nt) tsf protein chain elongation factor EF‑Ts
?AP012306 179956 = 440 (1.450)coding (239/852 nt) tsf protein chain elongation factor EF‑Ts
* ? AP012306 = 3567673196 (0.620)8 (0.030) 8/294 23.0 4.2% coding (576/1104 nt) gldA glycerol dehydrogenase, NAD
?AP012306 = 3567687 168 (0.540)coding (562/1104 nt) gldA glycerol dehydrogenase, NAD
* ? AP012306 2876782 =283 (0.900)7 (0.020) 7/274 23.0 2.6% coding (1324/1452 nt) panF pantothenate:sodium symporter
?AP012306 2876794 = 262 (0.910)coding (1336/1452 nt) panF pantothenate:sodium symporter
* ? AP012306 = 2275012240 (0.760)7 (0.020) 7/274 23.0 3.1% noncoding (60/76 nt) gltW tRNA‑Glu
?AP012306 3372719 = NA (NA)noncoding (72/76 nt) gltU tRNA‑Glu