breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 47.2% | 345.9 / 109.0 | 268 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 3,020,670 | 0 | T | G | 33.1% | 298.8 / 10.3 | 170 | K195N (AAA→AAC) | glgC | glucose‑1‑phosphate adenylyltransferase |
* | AP012306 | 644,417 | 0 | G | A | 31.4% | 711.8 / inf | 481 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 3,527,368 | 0 | A | C | 29.1% | 558.6 / 51.7 | 237 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 1,226,557 | 0 | A | G | 28.6% | 386.9 / 87.5 | 192 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 3,938,887 | 0 | C | T | 28.6% | 412.2 / 115.4 | 203 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 1,181,369 | 0 | A | C | 28.5% | 351.3 / 31.0 | 144 | K103N (AAA→AAC) | ycjS | predicted oxidoreductase, NADH‑binding |
* | AP012306 | 1,854,851 | 0 | A | C | 28.0% | 592.6 / 61.0 | 255 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 3,182,861 | 0 | A | C | 26.9% | 305.1 / 21.5 | 130 | L198F (TTA→TTC) | yiaN | predicted transporter |
* | AP012306 | 341,377 | 0 | T | G | 26.2% | 294.5 / 14.3 | 150 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 1,944,288 | 0 | A | C | 25.3% | 581.5 / 35.2 | 242 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 3,590,367 | 0 | A | C | 24.7% | 652.6 / 36.5 | 255 | T30P (ACC→CCC) | fabR | DNA‑binding transcriptional repressor |
* | AP012306 | 2,212,753 | 0 | A | C | 24.6% | 345.1 / 38.0 | 142 | Y241D (TAT→GAT) | hcaT | predicted 3‑phenylpropionic transporter |
* | AP012306 | 2,714,555 | 0 | T | G | 24.5% | 685.9 / 32.8 | 287 | T175P (ACC→CCC) | ygjV | conserved inner membrane protein |
* | AP012306 | 3,324,433 | 0 | T | A | 24.4% | 535.3 / 92.8 | 234 | intergenic (+2/+52) | yieF/yieG | chromate reductase, Class I, flavoprotein/predicted inner membrane protein |
* | AP012306 | 3,282,076 | 0 | T | G | 24.1% | 600.6 / 10.0 | 253 | intergenic (‑79/+27) | ilvB/ivbL | acetolactate synthase I, large subunit/ilvB operon leader peptide |
* | AP012306 | 935,054 | 0 | G | T | 24.0% | 346.2 / 59.2 | 121 | intergenic (+24/‑1406) | putP/ycdO | proline:sodium symporter/conserved protein |
* | AP012306 | 1,074,619 | 0 | T | C | 23.0% | 365.4 / 105.1 | 187 | S29G (AGC→GGC) | ldrA | toxic polypeptide, small |
* | AP012306 | 202,417 | 0 | T | G | 22.5% | 694.9 / 27.5 | 284 | V409G (GTA→GGA) | tilS | tRNA(Ile)‑lysidine synthetase |
* | AP012306 | 1,128,412 | 0 | T | G | 22.3% | 675.9 / 32.2 | 265 | V29G (GTG→GGG) | yciO | conserved protein |
Marginal new junction evidence (lowest skew 10 of 140 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | = 215364 | NA (NA) | 16 (0.050) | 16/288 | 19.1 | NA | noncoding (745/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) |
? | AP012306 | = 215372 | NA (NA) | noncoding (753/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) | |||||
* | ? | AP012306 | = 1092512 | 382 (1.220) | 14 (0.050) | 12/258 | 19.7 | 4.3% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 300 (1.110) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | 2424031 = | 322 (1.030) | 9 (0.030) | 9/270 | 21.6 | 2.9% | coding (156/2235 nt) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
? | AP012306 | 2424045 = | 303 (1.070) | coding (142/2235 nt) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase | |||||
* | ? | AP012306 | = 1092334 | 318 (1.010) | 8 (0.030) | 7/258 | 22.4 | 2.7% | coding (198/276 nt) | ychS | predicted protein |
? | AP012306 | 1092985 = | 299 (1.100) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 152555 | 308 (0.980) | 10 (0.030) | 9/292 | 22.4 | 3.2% | coding (3/480 nt) coding (1397/1398 nt) |
folK pcnB |
2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase poly(A) polymerase I |
? | AP012306 | = 152570 | 306 (0.990) | coding (1382/1398 nt) | pcnB | poly(A) polymerase I | |||||
* | ? | AP012306 | = 3959350 | 319 (1.020) | 9 (0.030) | 8/286 | 22.8 | 2.8% | intergenic (‑18/‑88) | ytjB/serB | conserved protein/3‑phosphoserine phosphatase |
? | AP012306 | = 3959401 | 325 (1.080) | intergenic (‑69/‑37) | ytjB/serB | conserved protein/3‑phosphoserine phosphatase | |||||
* | ? | AP012306 | 179936 = | 448 (1.430) | 8 (0.030) | 8/288 | 22.8 | 1.8% | coding (219/852 nt) | tsf | protein chain elongation factor EF‑Ts |
? | AP012306 | 179956 = | 440 (1.450) | coding (239/852 nt) | tsf | protein chain elongation factor EF‑Ts | |||||
* | ? | AP012306 | = 3567673 | 196 (0.620) | 8 (0.030) | 8/294 | 23.0 | 4.2% | coding (576/1104 nt) | gldA | glycerol dehydrogenase, NAD |
? | AP012306 | = 3567687 | 168 (0.540) | coding (562/1104 nt) | gldA | glycerol dehydrogenase, NAD | |||||
* | ? | AP012306 | 2876782 = | 283 (0.900) | 7 (0.020) | 7/274 | 23.0 | 2.6% | coding (1324/1452 nt) | panF | pantothenate:sodium symporter |
? | AP012306 | 2876794 = | 262 (0.910) | coding (1336/1452 nt) | panF | pantothenate:sodium symporter | |||||
* | ? | AP012306 | = 2275012 | 240 (0.760) | 7 (0.020) | 7/274 | 23.0 | 3.1% | noncoding (60/76 nt) | gltW | tRNA‑Glu |
? | AP012306 | 3372719 = | NA (NA) | noncoding (72/76 nt) | gltU | tRNA‑Glu |