breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG37.7% 580.2 / 66.6 301intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP012306341,3770TG35.8% 215.0 / 32.3 134G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123063,938,8870CT31.2% 361.2 / 131.2 199S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP012306644,4170GA30.0% 845.3 / inf 524intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,944,2880AC29.8% 509.0 / 49.3 218G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,182,8610AC29.8% 309.2 / 24.9 131L198F (TTA→TTCyiaNpredicted transporter
*AP0123061,854,8510AC29.6% 766.6 / 89.8 325T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP012306935,0540GT27.9% 208.3 / 50.0 79intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123062,758,9090TG27.4% 591.7 / 29.2 237G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123063,412,0490TG26.5% 842.4 / 36.8 352intergenic (+25/‑122)proM/aslBtRNA‑Pro/predicted regulator of arylsulfatase activity
*AP0123063,527,3680AC26.2% 1205.0 / 97.9 469L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123061,421,1470TG25.1% 507.5 / 11.2 231T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
*AP0123062,212,7530AC24.7% 413.7 / 34.7 154Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123061,128,4120TG24.5% 755.7 / 64.7 299V29G (GTG→GGG) yciOconserved protein
*AP0123062,714,5550TG24.1% 686.2 / 28.1 291T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP012306202,4170TG23.8% 839.3 / 37.8 328V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP012306800,6750AC23.4% 752.3 / 19.8 278T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123061,226,5570AG23.3% 450.6 / 66.6 193G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123061,074,6190TC23.2% 351.1 / 88.3 185S29G (AGC→GGC) ldrAtoxic polypeptide, small
*AP012306472,8680TG22.5% 547.6 / 11.1 200D435A (GAC→GCC) cusSsensory histidine kinase in two‑component regulatory system with CusR, senses copper ions

Marginal new junction evidence (lowest skew 10 of 174 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 1092512374 (1.190)17 (0.060) 11/258 19.0 5.3% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 278 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 997497271 (0.860)11 (0.040) 10/268 19.8 4.2% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 259 (0.920)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 1092334324 (1.030)10 (0.040) 9/258 20.0 3.5% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 278 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 643749440 (1.400)74 (0.350)
+48 bp
3/202 21.7 22.8% intergenic (+98/‑52) lysW/valZ tRNA‑Lys/tRNA‑Val
?AP012306 2073667 = 301 (0.960)noncoding (1/76 nt) lysV tRNA‑Lys
* ? AP012306 = 643749440 (1.400)74 (0.350)
+48 bp
3/202 21.7 21.1% intergenic (+98/‑52) lysW/valZ tRNA‑Lys/tRNA‑Val
?AP012306 644310 = 379 (1.200)noncoding (1/76 nt) lysQ tRNA‑Lys
* ? AP012306 = 643749440 (1.400)67 (0.310)
+48 bp
3/202 21.7 22.8% intergenic (+98/‑52) lysW/valZ tRNA‑Lys/tRNA‑Val
?AP012306 643880 = 231 (0.730)noncoding (1/76 nt) lysY tRNA‑Lys
* ? AP012306 643576 =302 (0.960)64 (0.300)
+48 bp
3/202 21.7 20.3% noncoding (1/76 nt) lysW tRNA‑Lys
?AP012306 = 643749 440 (1.400)intergenic (+98/‑52) lysW/valZ tRNA‑Lys/tRNA‑Val
* ? AP012306 643286 =379 (1.200)71 (0.330)
+47 bp
3/204 21.8 20.3% intergenic (+164/‑1) ybgF/lysT predicted protein/tRNA‑Lys
?AP012306 = 643749 440 (1.400)intergenic (+98/‑52) lysW/valZ tRNA‑Lys/tRNA‑Val
* ? AP012306 = 2524628318 (1.010)6 (0.020) 6/290 22.8 1.9% coding (1911/3099 nt) ygfK predicted oxidoreductase, Fe‑S subunit
?AP012306 = 2524656 305 (1.000)coding (1939/3099 nt) ygfK predicted oxidoreductase, Fe‑S subunit
* ? AP012306 2517709 =292 (0.930)6 (0.020) 6/292 22.8 2.0% coding (1024/1386 nt) hyuA D‑stereospecific phenylhydantoinase
?AP012306 2517723 = 290 (0.940)coding (1038/1386 nt) hyuA D‑stereospecific phenylhydantoinase