breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG47.2% 368.5 / 93.2 269intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC36.0% 456.9 / 44.1 228G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306644,4170GA33.4% 693.1 / inf 521intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,938,8870CT32.8% 437.9 / 181.9 259S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP012306341,3770TG30.4% 183.0 / 14.9 112G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123061,226,5570AG30.0% 332.7 / 83.9 171G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123063,527,3680AC29.1% 986.4 / 89.4 420L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306935,0540GT28.9% 179.5 / 45.5 76intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123063,020,6700TG27.7% 365.8 / 18.4 202K195N (AAA→AACglgCglucose‑1‑phosphate adenylyltransferase
*AP012306800,6750AC27.6% 585.7 / 21.1 252T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306202,4170TG27.3% 624.6 / 27.6 273V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123062,758,9090TG25.6% 534.9 / 12.0 199G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123063,282,0760TG25.4% 578.7 / 20.3 258intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP0123063,903,7910AG24.9% 537.4 / 63.5 233E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123061,854,8510AC24.8% 750.7 / 71.7 296T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP012306406,6770AG24.6% 507.1 / 24.5 199K294E (AAA→GAA) hemHferrochelatase
*AP0123063,182,8610AC24.1% 348.8 / 19.4 133L198F (TTA→TTCyiaNpredicted transporter
*AP0123062,558,0710AC21.9% 608.6 / 17.0 233V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123061,066,3230AC21.8% 669.2 / 14.8 229intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
*AP0123061,074,6190TC21.7% 380.0 / 99.9 198S29G (AGC→GGC) ldrAtoxic polypeptide, small

Marginal new junction evidence (lowest skew 10 of 86 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 3508410899 (2.750)16 (0.060) 15/254 11.1 1.9% intergenic (+759/‑94) yiiD/yiiE predicted acetyltransferase/predicted transcriptional regulator
?AP012306 3508849 = 904 (3.250)intergenic (+127/‑115) yiiE/yiiF predicted transcriptional regulator/conserved protein
* ? AP012306 = 1092512377 (1.150)16 (0.060) 11/258 12.4 4.9% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 289 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 643955370 (1.130)21 (0.070) 11/262 12.5 5.1% noncoding (76/76 nt) lysY tRNA‑Lys
?AP012306 644054 = 450 (1.570)intergenic (+99/‑48) lysY/lysZ tRNA‑Lys/tRNA‑Lys
* ? AP012306 = 1092334309 (0.950)11 (0.040) 9/258 13.1 3.8% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 289 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 236382314 (0.960)10 (0.030) 8/266 13.6 3.6% intergenic (+59/‑117) yafL/yafM predicted lipoprotein and C40 family peptidase/conserved protein
?AP012306 237024 = 255 (0.880)intergenic (+28/‑2736) yafM/dinB conserved protein/DNA polymerase IV
* ? AP012306 = 215364NA (NA)9 (0.030) 9/288 13.7 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 3804407386 (1.180)8 (0.030) 8/292 14.1 2.1% coding (353/1029 nt) yjeK predicted lysine aminomutase
?AP012306 = 3804422 359 (1.120)coding (338/1029 nt) yjeK predicted lysine aminomutase
* ? AP012306 2275542 =NA (NA)7 (0.020) 7/290 14.5 NA noncoding (1243/1542 nt) rrsG 16S ribosomal RNA (rrnG)
?AP012306 2275626 = NA (NA)noncoding (1159/1542 nt) rrsG 16S ribosomal RNA (rrnG)
* ? AP012306 644036 =511 (1.570)5 (0.020) 5/258 14.8 1.2% intergenic (+81/‑66) lysY/lysZ tRNA‑Lys/tRNA‑Lys
?AP012306 = 644159 377 (1.340)noncoding (58/76 nt) lysZ tRNA‑Lys
* ? AP012306 = 643365296 (0.910)6 (0.020) 5/256 14.8 1.7% intergenic (+3/‑133) lysT/valT tRNA‑Lys/tRNA‑Val
?AP012306 644057 = 433 (1.550)intergenic (+102/‑45) lysY/lysZ tRNA‑Lys/tRNA‑Lys