breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 47.2% | 368.5 / 93.2 | 269 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 1,944,288 | 0 | A | C | 36.0% | 456.9 / 44.1 | 228 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 644,417 | 0 | G | A | 33.4% | 693.1 / inf | 521 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 3,938,887 | 0 | C | T | 32.8% | 437.9 / 181.9 | 259 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 341,377 | 0 | T | G | 30.4% | 183.0 / 14.9 | 112 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 1,226,557 | 0 | A | G | 30.0% | 332.7 / 83.9 | 171 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 3,527,368 | 0 | A | C | 29.1% | 986.4 / 89.4 | 420 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 935,054 | 0 | G | T | 28.9% | 179.5 / 45.5 | 76 | intergenic (+24/‑1406) | putP/ycdO | proline:sodium symporter/conserved protein |
* | AP012306 | 3,020,670 | 0 | T | G | 27.7% | 365.8 / 18.4 | 202 | K195N (AAA→AAC) | glgC | glucose‑1‑phosphate adenylyltransferase |
* | AP012306 | 800,675 | 0 | A | C | 27.6% | 585.7 / 21.1 | 252 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 202,417 | 0 | T | G | 27.3% | 624.6 / 27.6 | 273 | V409G (GTA→GGA) | tilS | tRNA(Ile)‑lysidine synthetase |
* | AP012306 | 2,758,909 | 0 | T | G | 25.6% | 534.9 / 12.0 | 199 | G33G (GGT→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
* | AP012306 | 3,282,076 | 0 | T | G | 25.4% | 578.7 / 20.3 | 258 | intergenic (‑79/+27) | ilvB/ivbL | acetolactate synthase I, large subunit/ilvB operon leader peptide |
* | AP012306 | 3,903,791 | 0 | A | G | 24.9% | 537.4 / 63.5 | 233 | E189G (GAA→GGA) | yjgJ | predicted transcriptional regulator |
* | AP012306 | 1,854,851 | 0 | A | C | 24.8% | 750.7 / 71.7 | 296 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 406,677 | 0 | A | G | 24.6% | 507.1 / 24.5 | 199 | K294E (AAA→GAA) | hemH | ferrochelatase |
* | AP012306 | 3,182,861 | 0 | A | C | 24.1% | 348.8 / 19.4 | 133 | L198F (TTA→TTC) | yiaN | predicted transporter |
* | AP012306 | 2,558,071 | 0 | A | C | 21.9% | 608.6 / 17.0 | 233 | V302G (GTA→GGA) | visC | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
* | AP012306 | 1,066,323 | 0 | A | C | 21.8% | 669.2 / 14.8 | 229 | intergenic (‑92/+33) | ychM/prsA | predicted transporter/phosphoribosylpyrophosphate synthase |
* | AP012306 | 1,074,619 | 0 | T | C | 21.7% | 380.0 / 99.9 | 198 | S29G (AGC→GGC) | ldrA | toxic polypeptide, small |
Marginal new junction evidence (lowest skew 10 of 86 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | = 3508410 | 899 (2.750) | 16 (0.060) | 15/254 | 11.1 | 1.9% | intergenic (+759/‑94) | yiiD/yiiE | predicted acetyltransferase/predicted transcriptional regulator |
? | AP012306 | 3508849 = | 904 (3.250) | intergenic (+127/‑115) | yiiE/yiiF | predicted transcriptional regulator/conserved protein | |||||
* | ? | AP012306 | = 1092512 | 377 (1.150) | 16 (0.060) | 11/258 | 12.4 | 4.9% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 289 (1.020) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 643955 | 370 (1.130) | 21 (0.070) | 11/262 | 12.5 | 5.1% | noncoding (76/76 nt) | lysY | tRNA‑Lys |
? | AP012306 | 644054 = | 450 (1.570) | intergenic (+99/‑48) | lysY/lysZ | tRNA‑Lys/tRNA‑Lys | |||||
* | ? | AP012306 | = 1092334 | 309 (0.950) | 11 (0.040) | 9/258 | 13.1 | 3.8% | coding (198/276 nt) | ychS | predicted protein |
? | AP012306 | 1092985 = | 289 (1.020) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 236382 | 314 (0.960) | 10 (0.030) | 8/266 | 13.6 | 3.6% | intergenic (+59/‑117) | yafL/yafM | predicted lipoprotein and C40 family peptidase/conserved protein |
? | AP012306 | 237024 = | 255 (0.880) | intergenic (+28/‑2736) | yafM/dinB | conserved protein/DNA polymerase IV | |||||
* | ? | AP012306 | = 215364 | NA (NA) | 9 (0.030) | 9/288 | 13.7 | NA | noncoding (745/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) |
? | AP012306 | = 215372 | NA (NA) | noncoding (753/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) | |||||
* | ? | AP012306 | = 3804407 | 386 (1.180) | 8 (0.030) | 8/292 | 14.1 | 2.1% | coding (353/1029 nt) | yjeK | predicted lysine aminomutase |
? | AP012306 | = 3804422 | 359 (1.120) | coding (338/1029 nt) | yjeK | predicted lysine aminomutase | |||||
* | ? | AP012306 | 2275542 = | NA (NA) | 7 (0.020) | 7/290 | 14.5 | NA | noncoding (1243/1542 nt) | rrsG | 16S ribosomal RNA (rrnG) |
? | AP012306 | 2275626 = | NA (NA) | noncoding (1159/1542 nt) | rrsG | 16S ribosomal RNA (rrnG) | |||||
* | ? | AP012306 | 644036 = | 511 (1.570) | 5 (0.020) | 5/258 | 14.8 | 1.2% | intergenic (+81/‑66) | lysY/lysZ | tRNA‑Lys/tRNA‑Lys |
? | AP012306 | = 644159 | 377 (1.340) | noncoding (58/76 nt) | lysZ | tRNA‑Lys | |||||
* | ? | AP012306 | = 643365 | 296 (0.910) | 6 (0.020) | 5/256 | 14.8 | 1.7% | intergenic (+3/‑133) | lysT/valT | tRNA‑Lys/tRNA‑Val |
? | AP012306 | 644057 = | 433 (1.550) | intergenic (+102/‑45) | lysY/lysZ | tRNA‑Lys/tRNA‑Lys |