breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 1196 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA62.8% 32.6 / 88.7 87intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
*NC_000913357,8900CA59.7% ‑5.4 / 58.3 62L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_000913354,2880TG59.5% 45.0 / 100.9 74V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_000913501,5750AC58.8% 58.8 / 110.9 82V555G (GTG→GGG) ybaLinner membrane putative NAD(P)‑binding transporter
*NC_000913357,8800TC57.8% 30.8 / 87.0 64K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_0009134,604,3440CT56.9% 20.0 / 126.9 116S62F (TCT→TTT) bglJbgl operon transcriptional activator
*NC_0009131,356,2010TG54.5% 54.6 / 89.7 66T304P (ACC→CCC) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
*NC_0009133,665,7020CA53.7% 69.0 / 187.9 124A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
*NC_0009133,640,9810CT52.9% 14.3 / 102.0 85F40F (TTC→TTTdtpBdipeptide and tripeptide permease B
*NC_00091329,4960TA50.0% 51.5 / 83.6 82intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_0009134,604,3500CT50.0% 65.5 / 73.9 116S64F (TCT→TTT) bglJbgl operon transcriptional activator
*NC_000913357,8750AT49.2% 37.0 / 37.5 64N272K (AAT→AAAcynRtranscriptional activator of cyn operon; autorepressor
*NC_000913501,5720AC48.8% 106.3 / 13.8 82V556G (GTG→GGG) ybaLinner membrane putative NAD(P)‑binding transporter
*NC_000913354,2820TG48.6% 84.8 / 20.2 73V526G (GTT→GGT) prpEpropionate‑‑CoA ligase
*NC_0009134,065,5800TA48.1% 60.1 / 53.4 83T63S (ACC→TCC) yihOputative sulphoquinovose importer
*NC_0009134,065,5980GA48.1% 49.8 / 46.7 81L57L (CTG→TTG) yihOputative sulphoquinovose importer
*NC_000913827,5070TG47.9% 143.4 / 27.3 96D492A (GAC→GCC) ybhFputative ABC transporter ATPase
*NC_0009135,1590GA47.9% 39.6 / 51.7 72intergenic (+139/‑75)thrC/yaaXL‑threonine synthase/DUF2502 family putative periplasmic protein
*NC_0009131,554,0000TG47.5% 91.0 / 19.4 101Y557S (TAC→TCC) maeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
*NC_000913328,8070AT47.1% 29.7 / 38.2 53D176E (GAT→GAAbetIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor

Marginal new junction evidence (lowest skew 10 of 130 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 182887276 (0.740)8 (0.080) 8/278 8.0 10.6% coding (863/1479 nt) astD succinylglutamic semialdehyde dehydrogenase
?NC_000913 = 1828893 64 (0.670)coding (842/1479 nt) astD succinylglutamic semialdehyde dehydrogenase
* ? NC_000913 4409174 =NA (NA)8 (0.090) 7/266 8.1 5.5% noncoding (22/98 nt) RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 4409235 = 137 (1.490)noncoding (83/98 nt) RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 3216165 =90 (0.880)7 (0.070) 7/276 8.4 7.9% coding (327/765 nt) yqjH putative siderophore interacting protein
?NC_000913 3216247 = 80 (0.840)coding (245/765 nt) yqjH putative siderophore interacting protein
* ? NC_000913 = 1082873100 (0.980)7 (0.070) 7/276 8.4 6.8% coding (631/1128 nt) efeO inactive ferrous ion transporter EfeUOB
?NC_000913 = 1083021 98 (1.030)coding (779/1128 nt) efeO inactive ferrous ion transporter EfeUOB
* ? NC_000913 = 381689NA (NA)9 (0.090) 7/278 8.5 NA noncoding (430/1331 nt) IS2 repeat region
?NC_000913 = 381743 NA (NA)noncoding (484/1331 nt) IS2 repeat region
* ? NC_000913 3563404 =93 (0.910)8 (0.080) 7/282 8.6 7.9% coding (1392/1506 nt) glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
?NC_000913 3563422 = 97 (1.000)coding (1410/1506 nt) glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
* ? NC_000913 3927418 =105 (1.030)7 (0.070) 6/272 8.9 6.7% coding (264/993 nt) asnA asparagine synthetase A
?NC_000913 3927457 = 99 (1.050)coding (303/993 nt) asnA asparagine synthetase A
* ? NC_000913 = 2713829119 (1.160)6 (0.060) 6/274 8.9 5.1% coding (934/1335 nt) srmB ATP‑dependent RNA helicase
?NC_000913 = 2713908 115 (1.220)coding (1013/1335 nt) srmB ATP‑dependent RNA helicase
* ? NC_000913 4516891 =93 (0.910)8 (0.080) 6/278 9.0 8.7% coding (827/954 nt) fecR anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
?NC_000913 4516925 = 81 (0.840)coding (793/954 nt) fecR anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
* ? NC_000913 3619878 =NA (NA)8 (0.080) 6/278 9.0 100% coding (687/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3619892 = 0 (0.000)coding (701/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor