breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 156,056 T→G N49T (AAT→ACT)  Y75_RS00710 ← fimbrial chaperone
RA 547,694 A→G E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,835 +G coding (255/261 nt) Y75_RS02655 → hypothetical protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
MC JC 654,214 Δ1,199 bp Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
RA 666,783 A→T D619E (GAT→GAA mrdA ← peptidoglycan DD‑transpeptidase MrdA
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS porin OmpF/asparagine‑‑tRNA ligase
RA 1,093,686 T→C V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
RA 1,197,797 C→T H366H (CAC→CAT icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,809 C→T T370T (ACC→ACT icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,822 T→C L375M (TTA→CTG)  icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,824 A→G L375M (TTA→CTG icd → NADP‑dependent isocitrate dehydrogenase
MC JC 1,301,041 Δ1,336 bp Y75_RS06530Y75_RS06530 Y75_RS06530, Y75_RS06530
RA 1,459,205 G→A G354G (GGG→GGA paaK → phenylacetate‑CoA oxygenase/reductase subunit PaaK
RA 1,519,222 G→T V40V (GTG→GTT Y75_RS07640 → orphan toxin OrtT
RA 1,669,601 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,979,957 G→T intergenic (‑46/+398) flhD ← / ← Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← H48 family flagellin FliC
MC JC 2,176,983 Δ1,199 bp Y75_RS10980[gatA] Y75_RS10980, [gatA]
RA 2,245,455 A→G S283P (TCG→CCG)  Y75_RS11290 ← DUF418 family protein
MC JC 2,320,795 Δ1,336 bp Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,739,952 C→A intergenic (‑146/‑64) aroF ← / → Y75_RS13630 3‑deoxy‑7‑phosphoheptulonate synthase AroF/DUF2799 domain‑containing protein
RA 2,865,825 A→T pseudogene (383/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 2,866,111 A→G pseudogene (97/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 3,169,126 A→C T215P (ACA→CCA)  qseB → two‑component system response regulator QseB
RA 3,268,825 A→G T186A (ACA→GCA)  Y75_RS16240 → YhaC family protein
MC JC 3,742,936 Δ1,336 bp Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
MC JC 3,746,911 Δ1,199 bp Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) Y75_RS19595 → C4‑dicarboxylate transporter DctC
RA 3,975,201 G→T L199F (TTG→TTT gadX → acid resistance transcriptional activator GadX
MC JC 4,310,363 Δ1,199 bp Y75_RS21310[alsK] Y75_RS21310, [alsK]
RA 4,371,274 (A)5→3 pseudogene (179‑180/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter
RA 4,626,165 C→G C201W (TGC→TGG deoD → purine‑nucleoside phosphorylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1197834 1213126 15293 92 [90] [90] 91 [icd]–[Y75_RS23470] [icd],Y75_RS05945,Y75_RS05950,lit,Y75_RS05960,Y75_RS05965,Y75_RS24820,Y75_RS23460,Y75_RS05970,Y75_RS05975,Y75_RS05980,ymfL,Y75_RS23465,Y75_RS05995,Y75_RS06000,Y75_RS06005,Y75_RS06010,Y75_RS06015,Y75_RS06020,Y75_RS06025,Y75_RS06030,Y75_RS06035,pinE,Y75_RS06045,[Y75_RS23470]
* * ÷ NC_007779 2068445–2069342 2069342 1–898 92 [90] [90] 92 Y75_RS10470 IS5‑like element IS5 family transposase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 = 20563NA (NA)165 (1.070) 140/496 0.1 98.8% intergenic (‑55/+252) Y75_RS00100/rpsT IS1‑like element IS1A family transposase/30S ribosomal protein S20
?NC_007779 755210 = 2 (0.010)intergenic (‑7/‑374) Y75_RS24790/sdhC hypothetical protein/succinate dehydrogenase cytochrome b556 subunit
* ? NC_007779 289857 =154 (1.000)203 (1.330) 166/492 0.0 71.8% intergenic (+1/+16) Y75_RS23110/Y75_RS01415 DUF3987 domain‑containing protein/IS1 family transposase
?NC_007779 = 755217 7 (0.050)intergenic (‑14/‑367) Y75_RS24790/sdhC hypothetical protein/succinate dehydrogenase cytochrome b556 subunit
* ? NC_007779 380484 =NA (NA)135 (0.880) 124/496 0.4 NA coding (28/444 nt) Y75_RS01845 transferase
?NC_007779 = 381407 NA (NA)coding (518/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 2810717 =3 (0.020)157 (1.020) 134/496 0.2 98.1% coding (635/1173 nt) emrA multidrug efflux MFS transporter periplasmic adaptor subunit EmrA
?NC_007779 2995017 = NA (NA)intergenic (‑1/+11) Y75_RS24115/Y75_RS14910 type III secretion apparatus protein OrgA/MxiK/IS3‑like element IS2 family transposase
* ? NC_007779 = 28107213 (0.020)179 (1.160) 146/496 0.1 98.4% coding (639/1173 nt) emrA multidrug efflux MFS transporter periplasmic adaptor subunit EmrA
?NC_007779 = 2996347 NA (NA)pseudogene (1138/1140 nt) Y75_RS14925 hypothetical protein
* ? NC_007779 3128802 =NA (NA)172 (1.120) 129/496 0.3 98.3% coding (34/1137 nt) Y75_RS15525 hypothetical protein
?NC_007779 = 3189141 3 (0.020)coding (605/750 nt) Y75_RS15855 molecular chaperone
* ? NC_007779 = 3129996NA (NA)170 (1.100) 132/496 0.2 96.0% intergenic (+146/+1) Y75_RS15530/Y75_RS15535 IS5‑like element IS5 family transposase/lipopolysaccharide biosynthesis protein
?NC_007779 3189138 = 7 (0.050)coding (602/750 nt) Y75_RS15855 molecular chaperone
* ? NC_007779 = 3186081NA (NA)173 (1.130) 137/494 0.1 96.1% coding (26/2466 nt) Y75_RS15850 fimbrial biogenesis outer membrane usher protein
?NC_007779 3512753 = 7 (0.050)coding (529/1026 nt) cytR DNA‑binding transcriptional regulator CytR
* ? NC_007779 3188360 =6 (0.040)195 (1.270) 123/496 0.4 97.0% coding (2305/2466 nt) Y75_RS15850 fimbrial biogenesis outer membrane usher protein
?NC_007779 3746911 = NA (NA)intergenic (‑3/‑32) Y75_RS18580/Y75_RS18585 tryptophan permease/IS5‑like element IS5 family transposase
* ? NC_007779 = 31883643 (0.020)228 (1.490) 155/494 0.0 98.7% coding (2309/2466 nt) Y75_RS15850 fimbrial biogenesis outer membrane usher protein
?NC_007779 = 3262327 NA (NA)intergenic (+145/+4) Y75_RS16205/tdcD IS5‑like element IS5 family transposase/propionate kinase
* ? NC_007779 = 32611330 (0.000)179 (1.180) 137/488 0.1 100% intergenic (‑59/‑33) tdcE/Y75_RS16205 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase/IS5‑like element IS5 family transposase
?NC_007779 3262333 = 0 (0.000)pseudogene (1183/1185 nt) tdcD propionate kinase
* ? NC_007779 = 35127592 (0.010)191 (1.250) 140/494 0.1 99.0% coding (535/1026 nt) cytR DNA‑binding transcriptional regulator CytR
?NC_007779 = 3744262 NA (NA)intergenic (‑45/+31) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
* ? NC_007779 = 3744263NA (NA)147 (0.950) 127/496 0.3 99.3% intergenic (‑46/+30) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
?NC_007779 3903274 = 1 (0.010)coding (37/825 nt) Y75_RS19350 protein bax
* ? NC_007779 = 37505900 (0.000)162 (1.070) 142/488 0.1 100% intergenic (‑104/‑33) tnaA/Y75_RS18600 tryptophanase/IS5‑like element IS5 family transposase
?NC_007779 3751790 = 0 (0.000)intergenic (+151/+116) Y75_RS18600/tnaC IS5‑like element IS5 family transposase/tryptophanase leader peptide
* ? NC_007779 = 39032793 (0.020)184 (1.200) 151/494 0.0 98.4% coding (42/825 nt) Y75_RS19350 protein bax
?NC_007779 = 4504190 NA (NA)intergenic (+10/+167) Y75_RS22280/Y75_RS22285 IS3‑like element IS2 family transposase/phosphoethanolamine transferase
* ? NC_007779 4310363 =NA (NA)136 (0.880) 113/496 0.7 97.8% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 4546700 = 3 (0.020)intergenic (+461/‑17) fimB/fimE type 1 fimbria regulatory protein FimB/type 1 fimbria regulatory protein FimE
* ? NC_007779 = 4311556NA (NA)127 (0.830) 113/494 0.7 92.1% intergenic (+145/+1) Y75_RS21310/alsK IS5‑like element IS5 family transposase/allose kinase
?NC_007779 4540915 = 11 (0.070)intergenic (+204/+379) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 = 4311556NA (NA)164 (1.070) 130/494 0.2 98.2% intergenic (+145/+1) Y75_RS21310/alsK IS5‑like element IS5 family transposase/allose kinase
?NC_007779 = 4546704 3 (0.020)intergenic (+465/‑13) fimB/fimE type 1 fimbria regulatory protein FimB/type 1 fimbria regulatory protein FimE
* ? NC_007779 4547362 =56 (0.360)121 (0.820) 107/478 0.8 68.4% intergenic (+49/‑433) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 4547676 = 58 (0.390)intergenic (+363/‑119) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NC_007779 = 454737056 (0.360)117 (0.790) 103/478 0.9 67.6% intergenic (+57/‑425) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 = 4547666 58 (0.390)intergenic (+353/‑129) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA