breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 46,053 C→A R83S (CGC→AGC)  yaaU → putative transporter YaaU
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
JC JC 391,045 IS2 (–) +5 bp pseudogene (795‑799/1458 nt) yaiT → putative autotransporter YaiT
RA 492,312 G→T G74V (GGC→GTC) 
G74V (GGC→GTC) 
dnaX →
dnaX →
DNA polymerase III subunit tau
DNA polymerase III subunit tau
RA 804,606 C→A V368V (GTC→GTA ybhJ → putative hydratase YbhJ
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 891,331 C→G R50G (CGC→GGC)  nfsA → NADPH‑dependent nitroreductase NfsA
RA 1,440,603 G→T A61E (GCG→GAG)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,465,642 C→G pseudogene (251/2518 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,494,019 C→A T517N (ACT→AAT)  trg → methyl‑accepting chemotaxis protein Trg
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,293,064 C→A W614L (TGG→TTG)  ccmF ← holocytochrome c synthetase membrane subunit CcmF
RA 2,361,905 G→T L103M (CTG→ATG)  yfaX ← putative DNA‑binding transcriptional regulator YfaX
RA 2,754,531 G→T R78R (CGC→CGA ratA ← ribosome association toxin RatA
RA 2,801,113 G→T G50V (GGC→GTC)  nrdI → dimanganese‑tyrosyl radical cofactor maintenance flavodoxin NrdI
RA 2,883,764 C→A T125T (ACG→ACT casA ← type I‑E CRISPR system Cascade subunit CasA
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,484,252 C→A T12K (ACG→AAG)  yheU → UPF0270 family protein YheU
RA 3,505,231 C→A A264S (GCG→TCG)  yhfS ← putative aminotransferase YhfS
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
JC 3,646,247 Δ10 bp intergenic (+20/‑43) rlmJ → / → gor 23S rRNA m(6)A2030 methyltransferase/glutathione reductase (NADPH)
RA 3,815,835 C→T intergenic (‑67/+28) pyrE ← / ← rph orotate phosphoribosyltransferase/truncated RNase PH
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,145,883 G→T G630V (GGC→GTC)  pflD → putative formate acetyltransferase 2
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,281,187 G→T G403V (GGC→GTC)  yjcE → putative transporter YjcE
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,375,584 T→G D25A (GAT→GCT)  epmB ← lysine 2,3‑aminomutase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102991 3104059 1069 45 [41] [42] 45 [mutY] [mutY]
* * ÷ NC_000913 3423766–3424234 3424525–3424238 5–760 45 [42] [34] 43 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =54 (0.340)198 (1.420) 92/244 0.0 84.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 28 (0.200)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780554 (0.340)99 (0.710) 56/244 0.7 72.3% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 28 (0.200)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)211 (1.370) 91/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =277 (1.750)268 (1.700) 95/274 0.1 65.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)170 (1.070) 87/276 0.2 97.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA