breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 490,518 C→A D99Y (GAC→TAC)  priC ← primosomal replication protein N''
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,063,172 G→T P202T (CCG→ACG)  cbpA ← curved DNA‑binding protein
RA 1,198,918 C→T S75F (TCC→TTC)  lit → e14 prophage; cell death peptidase Lit
RA 1,377,672 G→T A281S (GCA→TCA)  ycjS → putative oxidoreductase YcjS
RA 1,423,989 C→A R70S (CGT→AGT)  trkG → Rac prophage; K(+) transporter TrkG
RA 1,549,842 C→A G814G (GGC→GGA fdnG → formate dehydrogenase N subunit alpha
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,803,277 G→T pseudogene (184/1900 nt) arpB → putative ankyrin repeat protein B, N‑terminal fragment
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,098,000 G→T S101Y (TCT→TAT)  wzzB ← regulator of length of O‑antigen component of lipopolysaccharide chains
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,788,446 A→G pseudogene (2050/2253 nt) ygaQ → putative uncharacterized protein YgaQ
RA 3,152,472 C→A G79G (GGC→GGA metC → cystathionine beta‑lyase/L‑cysteine desulfhydrase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,843,044 G→T A249E (GCG→GAG)  adeQ ← adenine transporter
RA 3,928,442 G→T Q388K (CAG→AAG)  viaA ← protein ViaA
RA 4,206,993 G→T F180L (TTC→TTA purH ← bifunctional AICAR transformylase/IMP cyclohydrolase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,305,274 G→T H442N (CAT→AAT)  yjcS ← alkyl sulfatase
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1097023–1097115 1097614 500–592 36 [35] [32] 36 [serX] [serX]
* * ÷ NC_000913 3102952 3104083 1132 37 [35] [35] 37 mutY mutY
* * ÷ NC_000913 3423749–3424234 3424533–3424239 6–785 37 [35] [35] 36 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =73 (0.570)67 (0.590) 40/244 1.3 51.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 60 (0.530)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780573 (0.570)31 (0.270) 27/244 2.5 33.2% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 60 (0.530)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)123 (0.990) 69/268 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =217 (1.700)180 (1.420) 73/274 0.2 62.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)152 (1.190) 75/276 0.2 96.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA