breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 781,068 G→T noncoding (1/76 nt) valZ → tRNA‑Val
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 945,728 C→A intergenic (‑171/‑143) ycaC ← / → ycaD putative hydrolase/putative transporter YcaD
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,996,342 G→T A164D (GCT→GAT)  sdiA ← DNA‑binding transcriptional dual regulator SdiA
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,439,543 G→T A193D (GCC→GAC)  yfcJ ← putative transporter YfcJ
RA 2,518,027 G→T intergenic (‑195/+14) pdeA ← / ← alaX putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala
RA 2,526,395 C→A V154V (GTG→GTT yfeR ← putative LysR‑type DNA‑binding transcriptional regulator YfeR
RA 2,606,471 G→T L70L (CTG→CTT hyfE → hydrogenase 4 component E
RA 3,115,418 C→A S1232I (AGC→ATC)  yghJ ← putative lipoprotein YghJ
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC JC 3,836,122 IS1 (–) +8 bp intergenic (‑193/‑93) yicJ ← / → selC putative xyloside transporter YicJ/tRNA‑Sec
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,405,840 G→T G385V (GGC→GTC)  purA → adenylosuccinate synthetase
RA 4,429,675 C→A intergenic (+120/‑189) fklB → / → cycA FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB/serine/alanine/glycine/cycloserine:H(+)symporter
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196270 1211433 15164 43 [38] [36] 42 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102950 3104055 1106 42 [41] [41] 42 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424234 3424525–3424238 5–756 47 [41] [34] 46 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)154 (1.110) 80/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =218 (1.520)197 (1.390) 81/274 0.2 63.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 8 (0.060)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)156 (1.090) 79/276 0.2 96.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 6 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA