breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,391,408 C→A R149L (CGT→CTT)  ycjY ← putative hydrolase YcjY
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,723,961 G→T G614V (GGC→GTC)  ydhK → putative transporter YdhK
RA 1,769,355 C→A G94G (GGC→GGA ydiK → putative transporter YdiK
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,907,522 G→T P24T (CCT→ACT)  yobF ← DUF2527 domain‑containing protein YobF
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,564,482 T→C intergenic (+110/‑41) yffR → / → yffS CPZ‑55 prophage; uncharacterized protein YffR/CPZ‑55 prophage; uncharacterized protein YffS
RA 3,174,218 G→T S393R (AGC→AGA parE ← DNA topoisomerase IV subunit B
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,666,866 C→A D239Y (GAC→TAC)  gadA ← glutamate decarboxylase A
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,916,862 A→G S172P (TCC→CCC)  atpD ← ATP synthase F1 complex subunit beta
RA 4,132,505 C→A intergenic (+238/‑111) metL → / → metF fused aspartate kinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,377,990 G→T P319T (CCC→ACC)  ampC ← beta‑lactamase
RA 4,452,576 G→T M2I (ATG→ATT ytfT → galactofuranose ABC transporter putative membrane subunit YtfT
RA 4,491,440 C→A A362A (GCG→GCT idnT ← L‑idonate/5‑ketogluconate/gluconate transporter
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter
RA 4,606,962 C→A G247C (GGT→TGT)  rsmC ← 16S rRNA m(2)G1207 methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196245 1211439 15195 65 [45] [48] 49 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102991 3104086 1096 51 [45] [48] 51 mutY mutY
* * ÷ NC_000913 3423768–3424234 3424525–3424238 5–758 49 [45] [31] 56 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)86 (0.570) 52/266 1.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =143 (0.920)158 (1.020) 77/272 0.3 68.1% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 6 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)182 (1.170) 78/274 0.3 97.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA