breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,264,930 A→G E406G (GAA→GGA)  hemA → glutamyl‑tRNA reductase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,045,328 C→A E192* (GAA→TAA)  ygfF ← putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
RA 3,281,284 G→C pseudogene (163/504 nt) agaA → putative truncated N‑acetylgalactosamine‑6‑phosphate deacetylase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,693,260 Δ11 bp coding (1967‑1977/2619 nt) bcsA ← cellulose synthase catalytic subunit
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC 3,919,003 (AGTGGAGGTAGAAAACGTCGCCCGGGAAT)1→2 coding (855/1542 nt) atpA ← ATP synthase F1 complex subunit alpha
RA 4,109,248 G→T V139F (GTT→TTT)  sbp → sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp
RA 4,233,852 C→T A32V (GCT→GTT)  pgi → glucose‑6‑phosphate isomerase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,392,311 C→A intergenic (+162/‑49) orn → / → glyV oligoribonuclease/tRNA‑Gly
RA 4,396,909 C→A S282Y (TCC→TAC)  amiB → N‑acetylmuramoyl‑L‑alanine amidase B

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 696755 696862 108 23 [18] [0] 52 glnW glnW
* * ÷ NC_000913 1196255 1211431 15177 25 [20] [21] 26 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102950 3104055 1106 24 [22] [22] 24 [mutY] [mutY]
* * ÷ NC_000913 3423768–3424233 3424531–3424238 6–764 24 [20] [22] 23 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 696863 =0 (0.000)46 (0.580)
+GAAGAAACAATC
21/252 2.2 49.7% intergenic (‑33/+2) glnW/glnU tRNA‑Gln/tRNA‑Gln
?NC_000913 = 697134 102 (1.170)intergenic (‑1/+379) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 12994980 (0.000)136 (1.600) 77/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =134 (1.530)102 (1.170) 59/274 0.2 60.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)104 (1.190) 63/276 0.1 97.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA