breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 248,194 G→T intergenic (+60/+164) rayT → / ← lfhA REP‑associated tyrosine transposase/flagellar system protein, promoterless fragment
RA 248,197 T→A intergenic (+63/+161) rayT → / ← lfhA REP‑associated tyrosine transposase/flagellar system protein, promoterless fragment
RA 248,205 C→A intergenic (+71/+153) rayT → / ← lfhA REP‑associated tyrosine transposase/flagellar system protein, promoterless fragment
RA 248,219 T→C intergenic (+85/+139) rayT → / ← lfhA REP‑associated tyrosine transposase/flagellar system protein, promoterless fragment
RA 248,221 T→C intergenic (+87/+137) rayT → / ← lfhA REP‑associated tyrosine transposase/flagellar system protein, promoterless fragment
RA 248,263 G→A intergenic (+129/+95) rayT → / ← lfhA REP‑associated tyrosine transposase/flagellar system protein, promoterless fragment
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 286,882 G→T E32* (GAG→TAG)  yagH → CP4‑6 prophage; putative xylosidase/arabinosidase
JC JC 584,565 IS1 (–) +9 bp intergenic (+135/+107) appY → / ← ompT DNA‑binding transcriptional activator AppY/outer membrane protease VII (outer membrane protein 3b)
RA 607,090 G→T G98G (GGC→GGA ybdK ← carboxylate‑amine ligase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
JC JC 1,646,627 IS5 (–) +4 bp coding (223‑226/333 nt) flxA → Qin prophage; protein FlxA
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,657,434 G→A S501F (TCT→TTT)  hscA ← iron‑sulfur cluster biosynthesis chaperone HscA
RA 2,911,176 Δ1 bp coding (164/249 nt) mazE ← antitoxin of the MazF‑MazE toxin‑antitoxin system MazE
RA 2,995,683 T→C pseudogene (338/707 nt) ygeN ← putative type III secretion system protein
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,760,398 C→A A284A (GCG→GCT selA ← selenocysteine synthase
RA 3,805,227 C→A E242* (GAA→TAA)  waaP ← lipopolysaccharide core heptose (I) kinase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,907,051 C→A M176I (ATG→ATT phoU ← negative regulator of the pho regulon
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,292,086 G→T R192L (CGC→CTC)  nrfE → putative cytochrome c‑type biogenesis protein NrfE
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196245 1211437 15193 19 [15] [15] 19 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102983 3104081 1099 17 [16] [16] 20 mutY mutY
* * ÷ NC_000913 3423759–3424233 3424536–3424239 7–778 17 [16] [16] 18 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)71 (1.290) 51/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =74 (1.310)83 (1.480) 56/274 0.0 68.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)50 (0.890) 36/276 0.4 92.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA