breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 323,187 G→T D284Y (GAT→TAT)  ykgF → putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain
RA 335,274 A→G intergenic (‑252/‑6) yahC ← / → yahD uncharacterized protein YahC/ankyrin repeat‑containing protein YahD
MC JC 471,472 Δ186 bp coding (837‑1022/1782 nt) mdlB → ABC transporter family protein MdlB
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,548,920 G→T S507I (AGC→ATC)  fdnG → formate dehydrogenase N subunit alpha
RA 1,676,155 G→T A69E (GCA→GAA)  pntB ← pyridine nucleotide transhydrogenase subunit beta
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC JC 2,104,153 IS1 (–) +9 bp coding (349‑357/1119 nt) wbbK ← putative lipopolysaccharide biosynthesis protein
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,312,350 C→A D134Y (GAC→TAC)  ompC ← outer membrane porin C
RA 2,488,221 G→T P316P (CCC→CCA yfdE ← acetyl‑CoA:oxalate CoA‑transferase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,412,281 C→A A210D (GCC→GAC)  yhdJ → DNA adenine methyltransferase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,804,999 (T)7→6 coding (118/936 nt) waaS ← lipopolysaccharide core biosynthesis protein
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,018,798 C→A intergenic (+38/‑57) rmuC → / → ubiE putative recombination limiting protein RmuC/bifunctional 2‑octaprenyl‑6‑methoxy‑1,4‑benzoquinone methylase and S‑adenosylmethionine:2‑DMK methyltransferase
MC JC 4,024,545 Δ12 bp coding (266‑277/489 nt) rfaH ← transcription antiterminator RfaH
RA 4,028,308 C→A A155A (GCG→GCT fadA ← 3‑ketoacyl‑CoA thiolase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,307,447 A→T L118Q (CTG→CAG)  alsK ← D‑allose kinase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102983 3104081 1099 13 [11] [12] 14 mutY mutY
* * ÷ NC_000913 3423770–3424234 3424525–3424238 5–756 14 [10] [10] 13 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =39 (0.840)13 (0.320) 12/246 2.2 26.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 38 (0.920)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780539 (0.840)11 (0.270) 11/246 2.4 23.3% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 38 (0.920)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)66 (1.460) 43/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =60 (1.290)86 (1.860) 48/276 0.0 74.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)49 (1.050) 35/278 0.3 96.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA