breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 130 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,549,4830GC66.7% 8.3 / 10.6 15intergenic (+51/+63)mdfA/ampGmultidrug MFS transporter/muropeptide permease AmpG
*NC_0029471,332,0760CT59.5% ‑6.2 / 43.4 42intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*NC_002947445,1080AT57.9% ‑6.4 / 19.5 19intergenic (+78/‑210)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase
*NC_002947699,7250AG53.3% ‑4.2 / 36.0 31intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*NC_002947445,1060CA52.6% ‑2.6 / 21.3 19intergenic (+76/‑212)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase
*NC_0029471,018,2010TG52.6% ‑3.7 / 17.3 19intergenic (+77/+54)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
*NC_002947445,1100TG50.0% 3.4 / 28.9 20intergenic (+80/‑208)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase
*NC_002947445,1180GA50.0% 1.1 / 21.2 18intergenic (+88/‑200)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase
*NC_0029474,353,6980AG50.0% 14.0 / 80.4 58intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*NC_0029475,490,1590TC50.0% ‑5.3 / 11.0 12intergenic (+68/‑79)purH/purDbifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase
*NC_002947195,6340CT48.8% 8.6 / 56.3 43T380T (ACC→ACTPP_0168putative surface adhesion protein
*NC_0029472,479,1350TC47.6% ‑0.4 / 22.9 21intergenic (‑48/+132)PP_2167/talPhoD‑like phosphatase/transaldolase
*NC_0029473,583,4150TA47.6% 7.3 / 10.1 22intergenic (+100/‑224)PP_3158/PP_5589hypothetical protein/rhs repeat‑associated core domain‑containing protein
*NC_002947445,1250CG47.1% 0.8 / 21.6 17intergenic (+95/‑193)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase
*NC_002947445,1260TC47.1% 2.8 / 18.3 17intergenic (+96/‑192)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase
*NC_002947445,1270CA47.1% 0.2 / 19.3 17intergenic (+97/‑191)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase
*NC_002947196,3400A.46.7% 40.4 / 54.2 30coding (1846/26049 nt)PP_0168putative surface adhesion protein
*NC_0029472,479,1400TC45.0% ‑0.5 / 20.9 21intergenic (‑53/+127)PP_2167/talPhoD‑like phosphatase/transaldolase
*NC_0029472,479,1540AG45.0% 2.0 / 19.5 20intergenic (‑67/+113)PP_2167/talPhoD‑like phosphatase/transaldolase
*NC_002947445,1230GA44.4% 7.7 / 16.8 18intergenic (+93/‑195)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase

Marginal new junction evidence (lowest skew 10 of 121 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 5066932 =56 (2.070)7 (0.260) 7/288 2.0 11.6% coding (612/1098 nt) PP_4459 transposase
?NC_002947 5066964 = 52 (1.960)coding (644/1098 nt) PP_4459 transposase
* ? NC_002947 = 95322326 (0.960)6 (0.230) 6/288 2.3 19.8% coding (29/624 nt) cyoC cytochrome bo terminal oxidase subunit III
?NC_002947 = 953241 23 (0.870)coding (47/624 nt) cyoC cytochrome bo terminal oxidase subunit III
* ? NC_002947 4962552 =31 (1.150)6 (0.250) 5/264 2.4 16.9% intergenic (‑41/+122) fliH/fliG flagellar assembly protein FliH/flagellar motor switch protein
?NC_002947 4962642 = 31 (1.280)intergenic (‑131/+32) fliH/fliG flagellar assembly protein FliH/flagellar motor switch protein
* ? NC_002947 = 185974227 (1.000)7 (0.280) 5/270 2.4 19.0% intergenic (+73/‑238) PP_1658/PP_1659 hypothetical protein/hypothetical protein
?NC_002947 = 1859773 35 (1.410)intergenic (+104/‑207) PP_1658/PP_1659 hypothetical protein/hypothetical protein
* ? NC_002947 = 79684333 (1.220)5 (0.210) 4/262 2.7 14.4% intergenic (+42/‑148) PP_0681/PP_0682 hypothetical protein/inner membrane protein
?NC_002947 = 796881 30 (1.240)intergenic (+80/‑110) PP_0681/PP_0682 hypothetical protein/inner membrane protein
* ? NC_002947 = 521524219 (0.700)4 (0.160) 4/274 2.8 17.1% intergenic (‑111/+67) rnd/PP_4592 ribonuclease D/metal dependent hydrolase
?NC_002947 = 5215279 21 (0.830)intergenic (‑148/+30) rnd/PP_4592 ribonuclease D/metal dependent hydrolase
* ? NC_002947 6039022 =20 (0.740)4 (0.150) 4/284 2.9 16.2% coding (340/1212 nt) dfp bifunctional 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase
?NC_002947 6039048 = 22 (0.840)coding (366/1212 nt) dfp bifunctional 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase
* ? NC_002947 5441919 =22 (0.810)4 (0.160) 4/280 2.9 17.4% coding (637/837 nt) PP_4775 hypothetical protein
?NC_002947 5441973 = 17 (0.660)coding (583/837 nt) PP_4775 hypothetical protein
* ? NC_002947 5115118 =33 (1.220)6 (0.230) 4/282 2.9 17.0% coding (585/1419 nt) aroP‑II aromatic amino acid transport protein
?NC_002947 5115157 = 27 (1.040)coding (546/1419 nt) aroP‑II aromatic amino acid transport protein
* ? NC_002947 4733512 =21 (0.780)5 (0.190) 4/284 2.9 19.5% coding (153/885 nt) sucD succinyl‑CoA synthetase subunit alpha
?NC_002947 4733544 = 21 (0.800)coding (121/885 nt) sucD succinyl‑CoA synthetase subunit alpha