breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 551 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913298,9600AC48.9% 103.8 / 36.4 89Y659D (TAT→GAT) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
*NC_00091329,4860CA47.7% 133.3 / 68.5 109intergenic (+291/‑165)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_00091329,4960TA46.2% 103.6 / 67.7 106intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_000913213,3410AG46.1% 101.9 / 29.0 76G337G (GGA→GGGtilStRNA(Ile)‑lysidine synthetase
*NC_0009132,434,8820TG45.5% 124.6 / 24.9 88Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_0009134,538,1090AC45.2% 136.1 / 29.9 84G219G (GGT→GGGnanMN‑acetylneuraminic acid mutarotase
*NC_0009131,622,8160GA44.1% 81.9 / 115.5 111intergenic (+75/+144)yneM/dgcZinner membrane‑associated protein/diguanylate cyclase, zinc‑sensing
*NC_0009132,603,3420AC43.8% 90.1 / 64.9 73T509P (ACC→CCC) hyfBhydrogenase 4, membrane subunit
*NC_0009132,421,4430AC43.5% 152.7 / 29.8 93Y40S (TAC→TCC) folXD‑erythro‑7,8‑dihydroneopterin triphosphate 2'‑epimerase and dihydroneopterin aldolase
*NC_0009133,850,0070GA43.4% 88.9 / 42.8 76P44S (CCG→TCG) uhpBsensory histidine kinase in two‑component regulatory sytem with UhpA
*NC_000913646,7470TG43.2% 136.9 / 12.1 81T255P (ACC→CCC) citG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
*NC_0009131,842,9140AG42.7% 102.1 / 30.7 77M182V (ATG→GTG) gdhAglutamate dehydrogenase, NADP‑specific
*NC_0009131,435,7270GA42.6% 147.9 / 78.9 116intergenic (‑108/+33)uspF/ompNstress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
*NC_000913869,1260TG42.2% 107.1 / 24.7 66V536G (GTG→GGG) gsiAglutathione ABC transporter ATPase
*NC_000913501,5750AC42.2% 161.0 / 15.5 85V555G (GTG→GGG) ybaLinner membrane putative NAD(P)‑binding transporter
*NC_000913925,9000AG41.8% 190.9 / 23.9 104noncoding (72/88 nt)serWtRNA‑Ser
*NC_00091315,4100TG41.7% 112.5 / 10.5 60intergenic (+112/‑35)dnaJ/insL1chaperone Hsp40, DnaK co‑chaperone/IS186 transposase
*NC_0009133,133,3020TG41.7% 182.8 / 18.8 97H219P (CAC→CCC) yghSputative ATP‑binding protein
*NC_0009134,378,8020AC41.3% 192.0 / 14.2 92V48G (GTA→GGA) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
*NC_0009131,649,2950TG41.2% 145.3 / 21.9 80intergenic (+254/‑314)ydfC/dicBuncharacterized protein, Qin prophage/Qin prophage; cell division inhibition protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 925901 =100 (1.070)26 (0.280)
+C
21/298 7.2 22.1% noncoding (71/88 nt) serW tRNA‑Ser
?NC_000913 = 1097399 84 (0.900)intergenic (+570/+166) ycdU/serX putative inner membrane protein/tRNA‑Ser
* ? NC_000913 925901 =100 (1.070)11 (0.120)
+C
6/298 14.0 13.7% noncoding (71/88 nt) serW tRNA‑Ser
?NC_000913 = 1097218 39 (0.420)intergenic (+389/+347) ycdU/serX putative inner membrane protein/tRNA‑Ser
* ? NC_000913 = 451468497 (1.040)4 (0.040) 4/296 15.4 4.1% coding (1994/2325 nt) fecA TonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein
?NC_000913 = 4514725 90 (0.970)coding (1953/2325 nt) fecA TonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein