breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 478,502 C→A D342E (GAC→GAA ndhF → NADH dehydrogenase (ubiquinone)
RA 584,121 G→T intergenic (+205/‑67) rplA → / → rplJ ribosomal protein L1/ribosomal protein L10
RA 707,909 A→T M1M (TTG→ATG) † mntB ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 973,213 C→A E175* (GAG→TAG)  yitU ← possible HAD superfamily hydrolase
RA 1,002,200 T→G M173R (ATG→AGG)  mecA2 → competence negative regulator MecA
RA 1,105,994 T→C T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase
RA 1,196,508 C→A intergenic (+2563/‑1229) ftsQ → / → ylmE cell division protein FtsQ/hypothetical protein
RA 1,232,053 A→T D233V (GAT→GTT)  prpC → possible PP2C protein phosphatase
RA 1,416,458 A→T I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,513,930 A→T L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,744,473 C→T A67T (GCT→ACT)  apt ← adenine phosphoribosyltransferase
RA 2,002,518 T→C noncoding (896/1540 nt) rrs3 ← 16S ribosomal RNA
RA 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,279,705 G→A intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,289,257 A→C V78G (GTA→GGA)  ybbP ← hypothetical membrane protein
RA 2,337,959 C→T intergenic (‑125/‑119) adhR ← / → USA300HOU_2196 possible MerR family transcriptional regulator/hypothetical protein
RA 2,455,692 Δ1 bp coding (1210/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,455,698 2 bp→AT coding (1216‑1217/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP000730 58367 58581 215 2 [1] [1] 2 [USA300HOU_0049] [USA300HOU_0049]
* * ÷ CP000730 58810 58989 180 2 [1] [1] 2 USA300HOU_0049/USA300HOU_0050 hypothetical protein/hypothetical protein
* * ÷ CP000730 85126 85218 93 2 [1] [1] 2 USA300HOU_0082/copB nickel (Ni2+)/peptide ABC superfamily ATP binding cassette transporter, ABC protein/P‑ATPase superfamily P‑type ATPase copper (Cu2+) transporter
* * ÷ CP000730 170875 170903 29 3 [1] [1] 2 USA300HOU_0160/adhE hypothetical protein/alcohol dehydrogenase
* * ÷ CP000730 611529 611623 95 3 [1] [1] 2 azo1/sdrC hypothetical protein/Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrC
* * ÷ CP000730 661200 661313 114 2 [1] [0] 2 USA300HOU_0602 hypothetical protein
* * ÷ CP000730 662708 662817 110 2 [1] [1] 2 USA300HOU_0605 hypothetical protein
* * ÷ CP000730 742725 742840 116 2 [1] [1] 2 yetJ/sarX hypothetical membrane protein/possible staphylococcal accessory protein X
* * ÷ CP000730 760815 760839 25 2 [0] [1] 2 [cobW2] [cobW2]
* * ÷ CP000730 1164471 1164570 100 3 [1] [1] 2 USA300HOU_1089/USA300HOU_1090 hypothetical protein/hypothetical protein
* * ÷ CP000730 1171580 1171700 121 2 [1] [1] 2 hly/USA300HOU_1100 alpha‑hemolysin/hypothetical protein
* * ÷ CP000730 1181503 1181602 100 3 [1] [1] 2 USA300HOU_1111/USA300HOU_t0016 hypothetical protein/tRNA‑Arg
* * ÷ CP000730 1295723 1295851 129 2 [1] [1] 2 rpsO/pnp ribosomal protein S15/polyribonucleotide nucleotidyltransferase
* * ÷ CP000730 1604524 1604655 132 2 [1] [1] 2 [USA300HOU_1489] [USA300HOU_1489]
* * ÷ CP000730 1885309 1885367 59 2 [1] [1] 2 fmtB1/USA300HOU_1748 cell surface anchored protein/possible rhodanese domain sulfurtransferase
* * ÷ CP000730 1934817 1934921 105 3 [1] [1] 2 USA300HOU_1798 hypothetical protein
* * ÷ CP000730 1960897 1960980 84 2 [1] [1] 2 USA300HOU_1820/USA300HOU_1821 hypothetical bacteriophage protein/hypothetical protein
* * ÷ CP000730 2055594 2055697 104 4 [1] [1] 2 purB/sspP adenylosuccinate lyase/C47 family staphopain protease
* * ÷ CP000730 2507013 2507105 93 2 [1] [1] 3 USA300HOU_2367/sarZ hypothetical protein/transcriptional regulator

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =16 (0.230)38 (0.750)
+40 bp
32/220 0.3 76.4% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 = 514218NA (NA)4 (0.060) 4/290 2.1 NA noncoding (1426/1540 nt) rrs1 16S ribosomal RNA
?CP000730 = 514270 NA (NA)noncoding (1478/1540 nt) rrs1 16S ribosomal RNA
* ? CP000730 = 515946NA (NA)3 (0.050) 3/286 2.3 NA noncoding (1241/2921 nt) rrl1 23S ribosomal RNA
?CP000730 = 515981 NA (NA)noncoding (1276/2921 nt) rrl1 23S ribosomal RNA
* ? CP000730 = 517446NA (NA)10 (0.150) 6/286 1.8 NA noncoding (2741/2921 nt) rrl1 23S ribosomal RNA
?CP000730 = 517450 NA (NA)noncoding (2745/2921 nt) rrl1 23S ribosomal RNA
* ? CP000730 = 200253412 (0.170)90 (2.110)
+57 bp
48/186 0.1 92.4% noncoding (880/1540 nt) rrs3 16S ribosomal RNA
?CP000730 2002593 = NA (NA)noncoding (821/1540 nt) rrs3 16S ribosomal RNA