breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 35,858 T→A 38.2% intergenic (+89/+19) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,876 C→T 52.5% intergenic (+107/+1) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,913 C→G 21.1% S213S (TCG→TCC tpn ← IS431mec transposase
RA 35,940 G→A 22.8% R204R (CGC→CGT tpn ← IS431mec transposase
RA 35,970 T→C 64.3% E194E (GAA→GAG tpn ← IS431mec transposase
RA 35,997 T→C 28.2% T185T (ACA→ACG tpn ← IS431mec transposase
RA 36,015 C→T 26.2% R179R (AGG→AGA tpn ← IS431mec transposase
RA 36,087 A→G 60.5% C155C (TGT→TGC tpn ← IS431mec transposase
RA 36,119 C→T 25.9% V145I (GTA→ATA)  tpn ← IS431mec transposase
RA 36,124 G→A 54.8% A143V (GCT→GTT)  tpn ← IS431mec transposase
RA 36,133 A→G 28.9% V140A (GTA→GCA)  tpn ← IS431mec transposase
RA 36,161 T→C 30.4% I131V (ATT→GTT)  tpn ← IS431mec transposase
RA 36,174 T→C 29.7% K126K (AAA→AAG tpn ← IS431mec transposase
RA 36,269 C→T 53.5% A95T (GCA→ACA)  tpn ← IS431mec transposase
RA 36,315 G→A 52.3% Y79Y (TAC→TAT tpn ← IS431mec transposase
RA 36,378 C→T 77.7% L58L (TTG→TTA tpn ← IS431mec transposase
RA 36,413 C→T 46.6% V47I (GTC→ATC)  tpn ← IS431mec transposase
RA 36,444 C→T 41.1% R36R (AGG→AGA tpn ← IS431mec transposase
RA 36,471 A→G 34.0% S27S (AGT→AGC tpn ← IS431mec transposase
RA 36,479 T→C 30.0% T25A (ACA→GCA)  tpn ← IS431mec transposase
RA 36,581 C→T 20.1% intergenic (‑30/‑493) tpn ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein
RA 76,180 C→T 44.9% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,185 G→A 21.6% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,265 C→T 25.1% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,295 T→C 55.4% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,322 G→A 57.6% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,353 G→A 51.2% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 111,512 A→G 36.1% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 39.2% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 195,286 T→A 100% intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 396,540 G→A 100% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 512,643 A→G 56.5% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 607,884 G→A 100% R209* (CGA→TGA)  dck ← deoxynucleoside kinase
RA 629,681 C→A 100% L84I (CTA→ATA)  hxlA → possible 3‑hexulose‑6‑phosphate synthase
RA 665,392 T→G 100% L79* (TTA→TGA)  iolS → possible acetytransferase
RA 707,910 A→G 100% intergenic (‑1/‑121) mntB ← / → sirR ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 722,155 A→T 11.1% K42N (AAA→AAT fhuC → iron (Fe3+) ABC superfamily ATP binding cassette transporter, ABC protein
RA 796,114 C→A 22.6% S345Y (TCT→TAT)  recQ1 → ATP‑dependent helicase RecQ
RA 987,095 G→T 10.8% G305C (GGT→TGT)  fabH2 → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase
RA 1,233,504 T→A 100% V470D (GTT→GAT)  prkC → non‑specific serine/threonine protein kinase
RA 1,292,117 G→A 13.3% E597K (GAA→AAA)  infB → translation initiation factor IF‑2
RA 1,416,458 A→T 100% I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,085 G→T 100% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,460,315 T→C 13.5% intergenic (‑347/+53) USA300HOU_1356 ← / ← ctpA possible acetyltransferase/S41A family carboxy‑terminal peptidase
RA 1,479,498 C→T 20.0% G7509D (GGT→GAT)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,744,362 G→A 100% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
RA 1,761,889 C→A 100% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,929,796 T→C 36.4% intergenic (‑879/‑289) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,797 G→A 35.9% intergenic (‑880/‑288) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,799 C→A 31.6% intergenic (‑882/‑286) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,804 A→G 34.6% intergenic (‑887/‑281) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,812 G→A 46.5% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,843 Δ1 bp 57.5% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,848 C→A 63.1% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,861 T→C 67.0% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,862:1 +T 61.1% intergenic (‑945/‑223) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865 A→C 62.2% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866 G→T 62.2% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,867 G→T 63.0% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,870 A→C 63.2% intergenic (‑953/‑215) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,872 G→A 63.1% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,873 C→A 60.3% intergenic (‑956/‑212) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,875 T→C 61.6% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,917 C→T 53.6% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,918 A→G 53.4% intergenic (‑1001/‑167) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,925 T→A 42.1% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,928 T→A 42.1% intergenic (‑1011/‑157) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,930 T→A 42.2% intergenic (‑1013/‑155) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,941 A→T 37.4% intergenic (‑1024/‑144) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,946 G→A 33.4% intergenic (‑1029/‑139) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,352,815 G→A 100% A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,454,626 A→T 100% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T 100% T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,712,693 T→A 11.1% Q70H (CAA→CAT isaA ← immunodominant antigen A

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =25 (0.900)16 (0.860)
+45 bp
12/186 NT 46.0% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 2176447 = 31 (1.120)noncoding (114/114 nt) rrf5 5S ribosomal RNA
* ? CP000730 895785 =27 (0.980)6 (0.250) 5/242 NT 23.2% coding (806/822 nt) metQ ABC superfamily ATP binding cassette transporter, binding protein
?CP000730 = 909740 16 (0.660)intergenic (‑663/‑371) ear/USA300HOU_0868 Ear protein/hypothetical protein
* ? CP000730 2085296 =30 (1.080)19 (0.760) 13/248 NT 42.7% intergenic (‑558/‑400) mapW2/USA300HOU_1944 cell surface protein MapW2/hypothetical protein
?CP000730 = 2128343 24 (0.970)intergenic (+57/+1062) int/hlgB bacteriophage integrase/possible leukocidin subunit