breseq  version 0.26.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
JC JC 573,890 IS5 (–) +4 bp 50.8% intergenic (+20/‑63) ylcG → / → quuD uncharacterized protein, DLP12 prophage/DLP12 prophage; putative antitermination protein
RA 657,541 G→T 53.9% intergenic (+40/+14) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
RA 1,519,739 T→C 6.0% L238P (CTT→CCT)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent
RA 1,529,218 C→T 100% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,907,258 C→T 6.6% G60S (GGT→AGT)  cspC ← stress protein, member of the CspA‑family
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,186,771 C→A 5.6% intergenic (+30/‑189) rcnA → / → rcnB membrane protein conferring nickel and cobalt resistance/periplasmic modulator of Ni and Co efflux
RA 2,911,534 Δ1 bp 45.8% coding (2118/2235 nt) relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 3,560,455 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,809 +A 8.7% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,952,371 C→T 6.0% V57V (GTC→GTT ilvM → acetolactate synthase 2 small subunit
RA 4,187,507 A→C 100% N720H (AAC→CAC)  rpoC → RNA polymerase, beta prime subunit
RA 4,296,190 A→G 11.0% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 12.9% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 32.8% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 22.6% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,303 A→G 13.6% intergenic (+509/+133) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,304 A→C 14.4% intergenic (+510/+132) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380 +C 92.6% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380 +G 92.6% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,326,088 T→G 9.4% intergenic (‑347/+311) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,326,141 T→C 5.4% intergenic (‑400/+258) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 83979 84294 316 39 [37] [32] 42 leuL/leuO leu operon leader peptide/global transcription factor
* * ÷ NC_000913 583056 584241 1186 40 [38] [37] 39 [tfaX]–[appY] [tfaX], [appY]
* * ÷ NC_000913 720795 721197 403 47 [37] [38] 40 [ybfK]–[kdpE] [ybfK], [kdpE]
* * ÷ NC_000913 1978493 1979676 1184 40 [38] [37] 41 insB1–insA insB1, insA

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =37 (0.390)20 (0.220) 19/514 NT 38.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 28 (0.310)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780532 (0.340)32 (0.360) 29/514 NT 52.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 28 (0.310)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)29 (0.310) 24/538 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1907448 =130 (1.390)5 (0.050) 4/538 NT 3.7% coding (144/144 nt) yobF DUF2527 family heat‑induced protein
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 19785020 (0.000)35 (0.380) 32/530 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein