breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 233 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913417,5330TG66.7% 3.7 / 28.5 51V131G (GTG→GGG) phoBresponse regulator in two‑component regulatory system with PhoR
*NC_0009133,862,6640CG56.1% ‑3.8 / 32.9 42pseudogene (940/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
*NC_0009132,151,6860GT46.4% 0.2 / 11.8 29intergenic (+40/+25)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
*NC_0009133,745,0030AG40.7% 82.1 / 17.4 59E67G (GAA→GGA) yiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
*NC_0009133,665,7020CA38.6% 131.7 / 38.1 70A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
*NC_0009131,554,0000TG38.5% 146.3 / 15.3 97Y557S (TAC→TCC) maeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
*NC_0009134,101,5140GT38.5% 135.3 / 53.6 78intergenic (+84/‑176)sodA/kdgTsuperoxide dismutase, Mn/2‑keto‑3‑deoxy‑D‑gluconate transporter
*NC_000913433,6490TG38.4% 87.4 / 22.3 99G65G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*NC_0009133,862,6670GA38.1% 42.6 / 15.0 42pseudogene (937/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
*NC_000913354,2880TG37.5% 93.4 / 18.5 48V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_0009132,684,7790AC36.5% 81.0 / 18.3 76G243G (GGT→GGGglyAserine hydroxymethyltransferase
*NC_0009132,326,4500TG36.3% 222.1 / 35.6 103G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009133,745,0190CG35.7% 55.6 / 16.5 56G72G (GGC→GGGyiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
*NC_0009133,206,4460CA34.7% 144.8 / 30.0 76intergenic (‑190/‑17)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
*NC_0009134,101,5390TA34.1% 190.2 / 26.1 86intergenic (+109/‑151)sodA/kdgTsuperoxide dismutase, Mn/2‑keto‑3‑deoxy‑D‑gluconate transporter
*NC_0009132,483,2680TA33.9% 120.0 / 26.4 65L24F (TTA→TTTemrKmultidrug resistance efflux pump membrane fusion protein
*NC_0009134,604,3440CT33.6% 243.3 / 66.6 134S62F (TCT→TTT) bglJbgl operon transcriptional activator
*NC_000913884,1840AG33.3% 107.3 / 10.6 75E171G (GAG→GGG) mdfAmultidrug efflux system protein
*NC_0009133,760,3840AC32.8% 110.8 / 10.1 65V289G (GTG→GGG) selAselenocysteine synthase
*NC_000913811,7210TG32.7% 226.4 / 18.9 110V67G (GTG→GGG) bioCmalonyl‑ACP O‑methyltransferase, SAM‑dependent

Marginal new junction evidence (lowest skew 10 of 17 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =220 (1.290)41 (0.260) 31/332 3.1 19.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 146 (0.940)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805214 (1.250)33 (0.210) 30/332 3.3 16.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 143 (0.920)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 566774257 (1.500)16 (0.090) 14/360 6.5 6.3% pseudogene (91/93 nt) renD pseudogene, DLP12 prophage;Phage or Prophage Related
?NC_000913 568036 = 223 (1.320)pseudogene (2/213 nt) renD pseudogene, DLP12 prophage;Phage or Prophage Related
* ? NC_000913 = 2595193146 (0.850)6 (0.040) 5/346 9.4 4.4% coding (667/2016 nt) tmcA elongator methionine tRNA (ac4C34) acetyltransferase
?NC_000913 = 2595247 123 (0.760)coding (613/2016 nt) tmcA elongator methionine tRNA (ac4C34) acetyltransferase
* ? NC_000913 141977 =NA (NA)5 (0.030) 5/346 9.4 NA intergenic (+10/+31) hpt/can hypoxanthine phosphoribosyltransferase/carbonic anhydrase
?NC_000913 142000 = NA (NA)intergenic (+33/+8) hpt/can hypoxanthine phosphoribosyltransferase/carbonic anhydrase
* ? NC_000913 88432 =213 (1.250)6 (0.040) 5/350 9.4 3.3% coding (405/1005 nt) cra transcriptional repressor‑activator for carbon metabolism
?NC_000913 88504 = 143 (0.870)coding (477/1005 nt) cra transcriptional repressor‑activator for carbon metabolism
* ? NC_000913 4519650 =202 (1.180)4 (0.020) 4/346 9.9 2.2% coding (675/987 nt) yjhU putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element
?NC_000913 4519762 = 166 (1.020)coding (563/987 nt) yjhU putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element
* ? NC_000913 = 4081273153 (0.900)4 (0.020) 4/346 9.9 2.6% coding (588/636 nt) fdoI formate dehydrogenase‑O, cytochrome b556 subunit
?NC_000913 = 4081294 160 (0.990)coding (567/636 nt) fdoI formate dehydrogenase‑O, cytochrome b556 subunit
* ? NC_000913 1323539 =188 (1.100)4 (0.020) 4/346 9.9 2.3% coding (320/882 nt) yciV PHP domain protein
?NC_000913 1323564 = 163 (1.000)coding (345/882 nt) yciV PHP domain protein
* ? NC_000913 716906 =205 (1.200)5 (0.030) 4/344 9.9 2.4% intergenic (‑36/+40) ybfI/potE pseudogene/putrescine/proton symporter: putrescine/ornithine antiporter
?NC_000913 716932 = 207 (1.280)intergenic (‑62/+14) ybfI/potE pseudogene/putrescine/proton symporter: putrescine/ornithine antiporter