Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A20 F4 I5 R1
|
61 |
40.1 |
2005705 |
98.2% |
1969602 |
131.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AE015451_phaM1 |
6,011,161 |
T→C |
intergenic (+90/+20) |
xpt CDS (Xanthine phosphoribosyltransferase) → / ← PP_5266 CDS (putative acetyl‑CoA hydrolase family protein) |
–/– |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AE015451_phaM1 | 6,011,161 | 0 | T | C | 100.0%
| 15.2
/ NA
| 6 | intergenic (+90/+20) | xpt CDS (Xanthine phosphoribosyltransferase)/PP_5266 CDS (putative acetyl‑CoA hydrolase family protein) | –/– |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/0); total (6/0) |
GTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTC > AE015451_phaM1/6011133‑6011288
|
gtgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCt > 2:118559/1‑144 (MQ=255)
gtgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCt > 2:118562/1‑144 (MQ=255)
gtgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGACCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAACGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCt > 2:118561/1‑144 (MQ=255)
tgGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTc > 2:290038/1‑144 (MQ=255)
tgGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACCGCATCCGCCCCAGGTGGCTTTCATACCCCTc > 2:290039/1‑144 (MQ=255)
cGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc > 4:64233/1‑94 (MQ=255)
|
GTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTC > AE015451_phaM1/6011133‑6011288
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
