Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F4 I1 R1	86	415.0	19624302	98.4%	19310313	137.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	6,011,161	T→C	intergenic (+90/+20)	xpt CDS (Xanthine phosphoribosyltransferase) → / ← PP_5266 CDS (putative acetyl‑CoA hydrolase family protein)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	6,011,161	0	T	C	99.6%	890.7 / NA	225	intergenic (+90/+20)	xpt CDS (Xanthine phosphoribosyltransferase)/PP_5266 CDS (putative acetyl‑CoA hydrolase family protein)	–/–
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (132/92); minor base G (0/1); total (132/93)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCC  >  AE015451_phaM1/6011026‑6011295

ccGGGTTAAGTCGATGTAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCcgcg                                                                                                                                     <  2:2172452/139‑1 (MQ=255)

ccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTGCGC‑GGCCACGCCCGCTCCCACAGGTGCAGCACAGGTCGCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCcgcg                                                                                                                                     <  2:2178023/139‑1 (MQ=255)

       aaGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTGGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGcc                                                                                                                              <  2:3863106/139‑1 (MQ=255)

           cGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGGCCCGCCCGCTCCCCCAGGGGCCGCCCCGGTCTCGAACGCTGTGCGGTACCTGTGGGTGGGGGCGTGCCCGGGAATGGCCGCa                                                                                                                           >  2:4206575/1‑139 (MQ=255)

                       gtgtggtCAGCTTCATCTAATAATCGCGACACGTGCTTCCC‑GGGCACGCCCGCTCCCACCGGTGCAGCACAGGTCTCGACCGCTGTGCCGAACCTGTGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                                                              <  2:9176230/139‑1 (MQ=37)

                       gtgtgggCAGCTTAAGCGAAAAAACCCGACACGGGCTTGGG‑GGCAACCCCCGCACCACCAGGTGCAGCACGGGGCGGGAAGGCGGGGCGGAACCTGGGGGAGGGGGGGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                                                              <  2:8764889/139‑1 (MQ=255)

                            gtCAGCTTCATCTAATAAACGCGACACCTCGTTCCC‑GGCAACGCCCGCTCCCACAGGTGCAGCACAGGTCAGGAACGGTGTGCCGAACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTgc                                                                                                         <  2:5402795/139‑1 (MQ=37)

                                       cGATTAAACGCGACACCTGCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACTGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCg                                                                                              <  2:1396043/139‑1 (MQ=255)

                                                 cgtctgcTGGTTCGC‑GGGCACGCCCGCTCCCACAGGTTCAGCTCAGGTATCGAACGCAGTCCCGTCCCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCTACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaacaa                                                                                    <  2:2583472/133‑1 (MQ=25)

                                                  gacaggtGCTTCGC‑GGGCACGCCCGCACCCACAGGTACAGAACTGGTCTCTAACGCTGTCCCTTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAg                                                                                   <  1:4384234/133‑1 (MQ=37)

                                                     acCTGCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACGTGTGGTAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAgcgc                                                                                <  2:9092754/139‑1 (MQ=255)

                                                       cggCTGCGC‑GGCCACCCCCGCACCCACCGGTCCAGAACTGTTCGCCACCGGGGGGCGGTACGTGTGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                              <  1:4674923/137‑1 (MQ=255)

                                                       cTGTTTCCC‑GGGAACCCCCCCACCCACGGGTCCTCAACAGGACACGAACGGTTTCCCTAACGTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                              <  1:510414/139‑1 (MQ=19)

                                                       cTGGTTCGG‑GGGCACGCCCGCTCCCACGGGGGCTCCACTGGTCTCGAACGCTGTGCGGTACCTGTGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                              <  1:1062895/139‑1 (MQ=255)

                                                       cTGCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGACTCGAACGCTGTCCCGTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                              <  1:1183096/139‑1 (MQ=255)

                                                             cgc‑gGGCCCGCCCGCGCCCCCAGGTGCAGCACAGGTCTCTAACGCTGTCCGGTACGTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                                                                        <  2:5517326/139‑1 (MQ=38)

                                                             cgc‑gGGCACGCCCGCACCCAGCGGTCCAGCACAGGTGTCGAACGCTGTGCCGTACCTGTGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                                                                        <  2:3782186/139‑1 (MQ=255)

                                                             cgc‑gGGCACGCCCGATCCCACGGGTGCAGCACAGGTCTCTAAGGCTGTGCCGTACGTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                                                                        <  1:187558/139‑1 (MQ=37)

                                                              cgggggCACGCCCGCTCGCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                                                                        <  2:9068483/136‑1 (MQ=255)

                                                                      cGCCCGCACCCACTGGTGCAGCACAGGACTCGAACGCTGAGCCGTACCTGTGGGTGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTcttctt                                                                <  2:440777/139‑1 (MQ=255)

                                                                      cGCCCGCACCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTcttctt                                                                <  2:2086749/139‑1 (MQ=255)

                                                                       gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAATCGCTg                                                                              <  1:3651972/124‑1 (MQ=255)

                                                                       gCCCGCTCCCACACGTGCAGCACAGGTCTAGAACGCTGTGCCGTACCTGTGGGACCGGGCGTTCCCGCGAATTGCCCCAACCCATTTATATGGTTTCCGCCAGCCCCGCCAACAAAAACCGCTGGTAAAGTTcttgttg                                                               >  2:3651845/1‑138 (MQ=255)

                                                                           gCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGc                                                           <  1:4116672/139‑1 (MQ=255)

                                                                               ccACAGGTGCAGCACAGGTCTCGAACGCGGTGCCGTACCTGTGGGAGCGGCCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCt                                                       <  2:3734412/139‑1 (MQ=255)

                                                                                 ccacgTGCACCACAGCACACCAACGCTGAGCCGAACCTGGGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                                     <  2:9484973/135‑1 (MQ=255)

                                                                                 acaGGTGCAGCACAGGTCTCGAACGGTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                                     <  1:2778865/139‑1 (MQ=255)

                                                                                 acaGGTGCAGCACAGGTCACGACCGCTGGGCGGAACCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                                     <  1:1355318/139‑1 (MQ=255)

                                                                                 acaGGTGCAGCACAGGAAGCCAACCCTGTCCGGAACCTGTGGCAGCGGGCGTCCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                                     <  2:8328470/139‑1 (MQ=255)

                                                                                 acaGGGGCACCACAGGTCAGGAACGCTCGGCCGAACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                                     <  2:946982/139‑1 (MQ=38)

                                                                                    gttGCAGCACAGGTCTCGAAGGGTGTGCCGTACCTGGGGCAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGc                                                  <  1:3812396/137‑1 (MQ=255)

                                                                                     gTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCt                                                 <  2:2747334/139‑1 (MQ=255)

                                                                                          gcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                                            >  1:5290314/1‑139 (MQ=255)

                                                                                          gcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                                            >  1:2910722/1‑139 (MQ=255)

                                                                                          gcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                                            >  1:2469565/1‑139 (MQ=255)

                                                                                          gcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                                            >  1:7091629/1‑139 (MQ=255)

                                                                                              aGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                                        >  2:2036374/1‑139 (MQ=255)

                                                                                              aGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                                        >  2:4136302/1‑139 (MQ=255)

                                                                                              aGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                                        >  1:9058907/1‑139 (MQ=255)

                                                                                              aGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                                        >  2:8701524/1‑139 (MQ=255)

                                                                                              aGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                                        >  2:2781714/1‑139 (MQ=255)

                                                                                                  ctcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCAt                                    >  1:8834859/1‑139 (MQ=255)

                                                                                                  ctcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCAt                                    >  2:1553017/1‑139 (MQ=255)

                                                                                                  ctcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGACCCTGCGGCTGCCCCAACTGCAt                                    >  2:5616473/1‑139 (MQ=255)

                                                                                                   tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGccc                                                                                                >  1:1470670/1‑78 (MQ=255)

                                                                                                   tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATc                                   >  2:3683587/1‑139 (MQ=255)

                                                                                                   tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATc                                   >  1:2738640/1‑139 (MQ=255)

                                                                                                   tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATc                                   >  2:4372918/1‑139 (MQ=255)

                                                                                                   tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATc                                   >  1:4275603/1‑139 (MQ=255)

                                                                                                   tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATc                                   >  1:8023585/1‑139 (MQ=255)

                                                                                                      aaCGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGc                                >  1:1309376/1‑139 (MQ=255)

                                                                                                        cGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGccc                              >  2:3667618/1‑139 (MQ=255)

                                                                                                          gtgggCCGTACGTGTGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCa                            <  1:2978391/135‑1 (MQ=255)

                                                                                                          cTGTGCCGTACCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCa                            <  2:7081211/139‑1 (MQ=255)

                                                                                                            gtgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAgg                          <  1:7616576/139‑1 (MQ=255)

                                                                                                             tgCCGTACCTGTGGGAGCGTGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt                         >  2:5953139/1‑139 (MQ=255)

                                                                                                             tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt                         >  2:2279209/1‑139 (MQ=255)

                                                                                                             tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt                         >  2:6189229/1‑139 (MQ=255)

                                                                                                             tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt                         >  1:6559655/1‑139 (MQ=255)

                                                                                                             tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt                         >  1:6563446/1‑139 (MQ=255)

                                                                                                             tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt                         >  1:2532687/1‑139 (MQ=255)

                                                                                                              gCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTg                        >  2:1227979/1‑139 (MQ=255)

                                                                                                              gCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTg                        >  2:6649859/1‑139 (MQ=255)

                                                                                                              gCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTg                        >  1:2149494/1‑139 (MQ=255)

                                                                                                              gCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTg                        >  2:8507481/1‑139 (MQ=255)

                                                                                                              gCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTg                        >  1:7704756/1‑139 (MQ=255)

                                                                                                                cGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGc                      >  1:4655029/1‑139 (MQ=255)

                                                                                                                cGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGc                      >  2:395782/1‑139 (MQ=255)

                                                                                                                cGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCCCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTggg                      >  1:3346228/1‑138 (MQ=255)

                                                                                                                     ctctgGGAGCGGGGGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCa                 <  2:7583871/136‑1 (MQ=255)

                                                                                                                     cTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTg                                                      >  1:5706529/1‑102 (MQ=255)

                                                                                                                       gtgGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATa               >  2:1901017/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                              >  1:6608512/1‑73 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                              <  2:6608512/73‑1 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                                                                        <  1:2390186/79‑1 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                                                                        >  2:2390186/1‑79 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCa                                     >  2:51123/1‑114 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt                         >  2:1757720/1‑126 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt                         <  1:1757720/126‑1 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  2:3276735/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  2:9314451/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  1:356813/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  1:9552676/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  1:8365431/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  1:3860511/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  1:5436544/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  2:8260175/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  2:7480467/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            <  2:3907508/139‑1 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  1:4330167/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  1:6474229/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  1:2301632/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  2:4585123/1‑139 (MQ=255)

                                                                                                                          ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            >  1:2178523/1‑139 (MQ=255)

                                                                                                                          ggACCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc            <  1:4029310/139‑1 (MQ=255)

                                                                                                                            aGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCt          >  2:3141656/1‑139 (MQ=255)

                                                                                                                            aGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCt          >  2:6322099/1‑139 (MQ=255)

                                                                                                                             gCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTc         >  1:2186806/1‑139 (MQ=255)

                                                                                                                               gggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGGTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCCTCCGCCCCAGGTGGCTTACATACCCCTCCg       >  2:7010375/1‑139 (MQ=255)

                                                                                                                               gggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAGCCCTCCg       >  2:1418496/1‑139 (MQ=255)

CCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCC  >  AE015451_phaM1/6011026‑6011295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCA  >  AE015451_phaM1/6011032‑6011301

taagtcggtgtatgtttttttgttaaggttcttctAATAAACGCGACACGTGGTGCGGGGGCACGCCCCCTCCCACAGGTGCACCACAGGTCACCAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTAT                                                                                                                       <  VH01111:79:AACJV3JHV:1:1509:54114:30288/118‑1 (MQ=60)

taagtcggtgtatGGTGGTGTGGTCAGGTACAGCGAATAAACGGTACACGTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGTTCTCGAACGGTGTGCCGAACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTAT                                                                                                                       <  VH01111:79:AACJV3JHV:1:2512:68183:39545/140‑1 (MQ=60)

                          tatattttaataaaaaacaccacgtgatacggggccaccccccaacccccagggccaccactggactcaaaccgtggccgtaACCTGTGGGAGCGGGGGTCCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAA                                                                                             <  VH01111:79:AACJV3JHV:1:2604:8309:23415/71‑1 (MQ=60)

                                gggattaatgcccacgggtgcggcggcgccccgcccgcaccgccagggccagcacagggccccaaccctgggcccaagCTGTGGGTGCGGGCGGGCCCGCGAATGGCCGCAACCCTTTTATCAGGTTGCCGCCAGCCCCGCCAACAACAAGCG                                                                                       <  VH01111:79:AACJV3JHV:1:2505:70815:50658/75‑1 (MQ=60)

                                 CTAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACTGGTGCAGCACAGGTCTCTAACGCTGTGCCGTACCTGGGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGC                                                                                      <  VH01111:79:AACJV3JHV:1:1507:54531:46910/153‑1 (MQ=60)

                                         CCCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCTGCACAGGTGTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCCCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAG                                                                              <  VH01111:79:AACJV3JHV:1:1111:70702:43691/153‑1 (MQ=60)

                                            GACAGGTGCTTCGCGGGCACGCCCGCACCCACAGGTACAGAACTGGTCTCTAACGCTGTCCCTTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTC                                                                           <  VH01111:79:AACJV3JHV:1:2509:16982:38977/153‑1 (MQ=60)

                                                 ctgtttcccgggaacccccccacccacgggtcctcaacaggacacgaacggtttccctaacgTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTT                                                                      <  VH01111:79:AACJV3JHV:1:1204:29100:2401/91‑1 (MQ=60)

                                                 cggctgcgcggccacccccgcacccaccggtccagaactgttcgccaccggggggcggtacgTGTGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTT                                                                      <  VH01111:79:AACJV3JHV:1:2605:51387:46834/91‑1 (MQ=60)

                                                 GTGCTTCGGGGGCACGCCCGCTCCCACAGGTCCAGCACAGGTCTCCAACGCTGTGCCGTACGTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAATCGCTGGTAAAGTTCTTCTT                                                                      <  VH01111:79:AACJV3JHV:1:2312:71080:9463/153‑1 (MQ=60)

                                                 GTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAATCGCTGGTAAAGTTCTTCTT                                                                      <  VH01111:79:AACJV3JHV:1:2312:69755:9728/153‑1 (MQ=60)

                                                 GTGCTTCGCGGGCACGCCCGCACCCACAGGTGCAGCACAGGACTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTT                                                                      <  VH01111:79:AACJV3JHV:1:2609:11017:11318/153‑1 (MQ=60)

                                                 CTGGTTCGGGGGCACGCCCGCTCCCACGGGGGCTCCACTGGTCTCGAACGCTGTGCGGTACCTGTGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTT                                                                      <  VH01111:79:AACJV3JHV:1:1308:72576:22279/153‑1 (MQ=60)

                                                 CTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTT                                                                      <  VH01111:79:AACJV3JHV:1:1312:46994:20784/153‑1 (MQ=60)

                                                 CTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGACTCGAACGCTGTCCCGTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTT                                                                      <  VH01111:79:AACJV3JHV:1:1312:43756:20159/153‑1 (MQ=60)

                                                 CTGCTGCGCGGGCACCCCCGCTCCCACAGGTGCAGCACAGGTCGCGAACGCTGTCCCGTACCTGTGGGAGCGGGCGTCCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCca                                                                      <  VH01111:79:AACJV3JHV:1:2114:58280:1057/153‑3 (MQ=60)

                                                      TCGCGGGCACCCCCGCTCCCACAGGGGCACCACAGGACTCGAACGCTGTGCCTTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGC                                                                 <  VH01111:79:AACJV3JHV:1:1209:55534:32086/153‑1 (MQ=60)

                                                       ggcgggaacgccggtaccaaggggtgcagcactggtgtgtaacgggGTGCCGTCCCTGTGGGTGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCC                                                                <  VH01111:79:AACJV3JHV:2:1603:68145:9481/107‑1 (MQ=60)

                                                       ataacaggtcttgtctcgtgggctcggagatgtgtataagagacagGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCC                                                                <  VH01111:79:AACJV3JHV:1:1610:43737:24267/107‑1 (MQ=60)

                                                       CGCGGGCACGCCGGCTCCCACTGGTGCTGCACTGGTCTCTAAGGGTGTGCGTTACGTGTGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCC                                                                <  VH01111:79:AACJV3JHV:1:1503:75757:39129/153‑1 (MQ=60)

                                                       CGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGACTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCC                                                                <  VH01111:79:AACJV3JHV:1:1114:65381:25574/153‑1 (MQ=60)

                                                       CGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGACGCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCC                                                                <  VH01111:79:AACJV3JHV:1:2208:60704:50507/153‑1 (MQ=60)

                                                       CGCGGGCACGCCCGATCCCACGGGTGCAGCACAGGTCTCTAAGGCTGTGCCGTACGTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCC                                                                <  VH01111:79:AACJV3JHV:1:1107:70039:18493/153‑1 (MQ=60)

                                                       CGCGGCCACGCCGGCACCCACCGGTGCAGCACAGGACTCGACCGCTGTCCCGAACCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTAATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCC                                                                <  VH01111:79:AACJV3JHV:1:2109:60438:28508/153‑1 (MQ=60)

                                                                    GCTCCCACAGTTGCAGCACAGGTCTCGAACGGTGTGCCGTACGTGTGGGAGGGGGGGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCC                                                   <  VH01111:79:AACJV3JHV:1:1303:49267:51453/153‑1 (MQ=60)

                                                                    GCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCTAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCC                                                   <  VH01111:79:AACJV3JHV:1:2414:54474:21106/153‑1 (MQ=60)

                                                                    GCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCGGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCC                                                   <  VH01111:79:AACJV3JHV:1:1311:11206:25706/153‑1 (MQ=60)

                                                                    GCACCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTCCCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCC                                                   <  VH01111:79:AACJV3JHV:1:1113:19670:21257/153‑1 (MQ=60)

                                                                     CTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC                                                  >  VH01111:79:AACJV3JHV:2:1603:44438:31783/1‑153 (MQ=60)

                                                                     CTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC                                                  >  VH01111:79:AACJV3JHV:2:1603:44305:31726/1‑153 (MQ=60)

                                                                     CTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC                                                  >  VH01111:79:AACJV3JHV:2:1112:66725:42101/1‑153 (MQ=60)

                                                                     CTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC                                                  >  VH01111:79:AACJV3JHV:1:2114:43870:19932/1‑153 (MQ=60)

                                                                     CTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC                                                  >  VH01111:79:AACJV3JHV:1:1612:47108:56262/1‑153 (MQ=60)

                                                                     CTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC                                                  >  VH01111:79:AACJV3JHV:1:2114:43529:19856/1‑153 (MQ=60)

                                                                       CCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAA                                                >  VH01111:79:AACJV3JHV:2:1208:75625:15899/1‑153 (MQ=60)

                                                                         CACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACT                                              >  VH01111:79:AACJV3JHV:1:1608:61291:7550/1‑153 (MQ=60)

                                                                         CACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACT                                              >  VH01111:79:AACJV3JHV:2:2502:5715:29985/1‑153 (MQ=60)

                                                                         CACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACT                                              >  VH01111:79:AACJV3JHV:1:1608:53906:8308/1‑153 (MQ=60)

                                                                         CACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAAAAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACT                                              >  VH01111:79:AACJV3JHV:1:2413:35368:7494/1‑153 (MQ=60)

                                                                          accgggccagaactgtactggaagggtggGCCGTACGTGTGGAAGCGGGCGTCCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTG                                             <  VH01111:79:AACJV3JHV:1:2310:20636:14120/124‑1 (MQ=60)

                                                                          ACAGGTGCAGCACAGGTCTCGAACGGTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTG                                             <  VH01111:79:AACJV3JHV:1:2109:32565:42972/153‑1 (MQ=60)

                                                                          ACAGGTGCAGCACAGGTCACGACCGCTGGGCGGAACCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTG                                             <  VH01111:79:AACJV3JHV:1:1404:37375:6433/153‑1 (MQ=60)

                                                                             ggtacggcacagcgttcgagacctgtgctgcACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCAT                                          >  VH01111:79:AACJV3JHV:1:1305:46767:23548/32‑153 (MQ=60)

                                                                             GTTGCAGCACAGGTCTCGAAGGGTGTGCCGTACCTGGGGCAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCNACTGCAT                                          <  VH01111:79:AACJV3JHV:1:2403:56481:43218/153‑1 (MQ=60)

                                                                             GGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGTCCCTGCGGCTGCCCCAACTGCAT                                          >  VH01111:79:AACJV3JHV:1:2104:21867:33638/1‑153 (MQ=60)

                                                                             GGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCAT                                          >  VH01111:79:AACJV3JHV:2:2408:6472:31348/1‑153 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCctgtctcttatacacatctccgagtccacgagacaagacctgttatc                                         >  VH01111:79:AACJV3JHV:1:1407:25862:49447/1‑106 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATC                                         >  VH01111:79:AACJV3JHV:2:1410:50043:31859/1‑153 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATC                                         >  VH01111:79:AACJV3JHV:1:2302:43813:55012/1‑153 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATC                                         >  VH01111:79:AACJV3JHV:1:2506:32225:13476/1‑153 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATC                                         >  VH01111:79:AACJV3JHV:1:2501:44760:41533/1‑153 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATC                                         >  VH01111:79:AACJV3JHV:1:2505:35046:51775/1‑153 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATC                                         >  VH01111:79:AACJV3JHV:2:2207:15524:50734/1‑153 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATC                                         >  VH01111:79:AACJV3JHV:1:1612:25332:23018/1‑153 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATC                                         >  VH01111:79:AACJV3JHV:2:2608:33891:7229/1‑153 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATC                                         >  VH01111:79:AACJV3JHV:1:2108:36902:23112/1‑153 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAA                                                                                                                                         >  VH01111:79:AACJV3JHV:1:1611:44741:36327/1‑57 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAA                                                                                                                                         >  VH01111:79:AACJV3JHV:1:1209:27055:7096/1‑57 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAA                                                                                                                                         >  VH01111:79:AACJV3JHV:1:2406:34648:51643/1‑57 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAA                                                                                                                                         >  VH01111:79:AACJV3JHV:1:1410:16527:26066/1‑57 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAA                                                                                                                                         >  VH01111:79:AACJV3JHV:1:1410:16508:30363/1‑57 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAA                                                                                                                                         >  VH01111:79:AACJV3JHV:1:1209:27699:8308/1‑57 (MQ=60)

                                                                              GTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAA                                                                                                                                         >  VH01111:79:AACJV3JHV:1:2114:9294:38750/1‑57 (MQ=60)

                                                                                 CAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGC                                      >  VH01111:79:AACJV3JHV:1:1402:35784:30704/1‑153 (MQ=60)

                                                                                 CAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGC                                      >  VH01111:79:AACJV3JHV:1:1402:33721:32654/1‑153 (MQ=60)

                                                                                        GGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGT                               >  VH01111:79:AACJV3JHV:2:1413:52618:28489/1‑153 (MQ=60)

                                                                                        GGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGT                               >  VH01111:79:AACJV3JHV:2:1413:57257:35210/1‑153 (MQ=60)

                                                                                        GGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGT                               >  VH01111:79:AACJV3JHV:1:2101:55724:8573/1‑153 (MQ=60)

                                                                                        GGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGT                               >  VH01111:79:AACJV3JHV:1:2101:55875:8421/1‑153 (MQ=60)

                                                                                        GGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGT                               >  VH01111:79:AACJV3JHV:2:1413:57371:35475/1‑153 (MQ=60)

                                                                                         GTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTG                              >  VH01111:79:AACJV3JHV:2:2110:65778:37330/1‑153 (MQ=60)

                                                                                         GTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTG                              >  VH01111:79:AACJV3JHV:1:1602:7419:26653/1‑153 (MQ=60)

                                                                                         GTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTG                              >  VH01111:79:AACJV3JHV:2:1609:31448:53858/1‑153 (MQ=60)

                                                                                         GTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTG                              >  VH01111:79:AACJV3JHV:1:1304:39988:41912/1‑153 (MQ=60)

                                                                                          ataagagacagGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGG                             <  VH01111:79:AACJV3JHV:1:1102:13403:40094/142‑1 (MQ=60)

                                                                                          TCTCGAACGCTGTCCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGG                             <  VH01111:79:AACJV3JHV:1:1510:16679:11072/153‑1 (MQ=60)

                                                                                           CTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGC                            >  VH01111:79:AACJV3JHV:1:2605:16357:11734/1‑153 (MQ=60)

                                                                                           CTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGC                            >  VH01111:79:AACJV3JHV:2:1412:58640:29625/1‑153 (MQ=60)

                                                                                           CTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGC                            >  VH01111:79:AACJV3JHV:1:1404:42696:39810/1‑153 (MQ=60)

                                                                                           CTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCCCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGG                            >  VH01111:79:AACJV3JHV:1:2301:71099:25763/1‑153 (MQ=60)

                                                                                                ACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTctgtctcttatacacatctccga                       >  VH01111:79:AACJV3JHV:1:1603:46862:28640/1‑130 (MQ=60)

                                                                                                ACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTctgtctcttatacacatctccga                       >  VH01111:79:AACJV3JHV:2:1212:60230:40037/1‑130 (MQ=60)

                                                                                                ACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCA                       >  VH01111:79:AACJV3JHV:2:2412:18080:10580/1‑153 (MQ=60)

                                                                                                   gtggGCCGTACGTGTGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAC                    <  VH01111:79:AACJV3JHV:1:2202:68789:38864/149‑1 (MQ=60)

                                                                                                   ggggccggaccgtggGGGAGGGGGCGTCCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAC                    <  VH01111:79:AACJV3JHV:1:2301:55099:16808/138‑1 (MQ=60)

                                                                                                   ctggcccgaacgTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAC                    <  VH01111:79:AACJV3JHV:1:2605:60420:18342/141‑1 (MQ=60)

                                                                                                   CTGTGCCGAACGTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAC                    <  VH01111:79:AACJV3JHV:1:2404:67388:32313/153‑1 (MQ=60)

                                                                                                   CGGTGCCGTACCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAC                    <  VH01111:79:AACJV3JHV:1:1412:76382:21995/153‑1 (MQ=60)

                                                                                                     ttccggaacgtgaGGGAGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  <  VH01111:79:AACJV3JHV:1:2203:33607:38371/140‑1 (MQ=60)

                                                                                                     GTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:2:2605:58412:48557/1‑153 (MQ=60)

                                                                                                     GTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:1607:35311:29019/1‑153 (MQ=60)

                                                                                                     GTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:2104:25218:51226/1‑153 (MQ=60)

                                                                                                     GTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:2:1410:12134:30344/1‑153 (MQ=60)

                                                                                                     GTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:2:1203:42961:37690/1‑153 (MQ=60)

                                                                                                     GTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:1214:36940:28830/1‑153 (MQ=60)

                                                                                                     GTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:1112:45536:48935/1‑153 (MQ=60)

                                                                                                     GTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:2313:44230:3329/1‑153 (MQ=60)

                                                                                                     GTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:1204:56500:49939/1‑153 (MQ=60)

                                                                                                     GTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:2507:15277:46550/1‑153 (MQ=60)

                                                                                                     GTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTctgtctcttatacacatctccgagcccacgagacaagacctgttatctggta                  >  VH01111:79:AACJV3JHV:2:1501:51690:12567/1‑101 (MQ=60)

                                                                                                     GTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTctgtctcttatacacatctccgagcccacgagacaagacctgttatctcgta                  >  VH01111:79:AACJV3JHV:2:1501:28097:9500/1‑101 (MQ=60)

                                                                                                     GTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCCGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:2:2306:49948:2420/1‑153 (MQ=60)

                                                                                                     GTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:1603:67009:25460/1‑153 (MQ=60)

                                                                                                     GTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:1603:64112:24570/1‑153 (MQ=60)

                                                                                                     GTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  <  VH01111:79:AACJV3JHV:2:2107:65930:30590/153‑1 (MQ=60)

                                                                                                     GTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:1405:19954:24116/1‑153 (MQ=60)

                                                                                                     GTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:2405:15202:27467/1‑153 (MQ=60)

                                                                                                     GTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:1203:7249:23680/1‑153 (MQ=60)

                                                                                                     GTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCC                  >  VH01111:79:AACJV3JHV:1:1214:37811:35342/1‑153 (MQ=60)

                                                                                                      TGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCC                 <  VH01111:79:AACJV3JHV:1:1503:58640:23150/153‑1 (MQ=60)

                                                                                                       gctgcACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCT                >  VH01111:79:AACJV3JHV:1:2509:59454:36270/6‑153 (MQ=60)

                                                                                                       GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGctgtctcttataca                >  VH01111:79:AACJV3JHV:1:2414:61707:51207/1‑139 (MQ=60)

                                                                                                        CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTC               >  VH01111:79:AACJV3JHV:1:1603:41882:39602/1‑153 (MQ=60)

                                                                                                        CCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTC               >  VH01111:79:AACJV3JHV:1:1603:41484:39621/1‑153 (MQ=60)

                                                                                                            ACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGC           >  VH01111:79:AACJV3JHV:2:2110:59700:29473/1‑153 (MQ=60)

                                                                                                            ACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGC           >  VH01111:79:AACJV3JHV:2:2112:30937:35967/1‑153 (MQ=60)

                                                                                                            ACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGC           >  VH01111:79:AACJV3JHV:2:2311:15997:47156/1‑153 (MQ=60)

                                                                                                                   GGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTG    <  VH01111:79:AACJV3JHV:1:2514:38019:43464/153‑1 (MQ=60)

                                                                                                                   GGACCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTG    <  VH01111:79:AACJV3JHV:1:2411:48414:18872/153‑1 (MQ=60)

                                                                                                                    GAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGC   >  VH01111:79:AACJV3JHV:2:1111:43472:46645/1‑153 (MQ=60)

                                                                                                                    GAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGC   >  VH01111:79:AACJV3JHV:2:1111:43889:46266/1‑153 (MQ=60)

                                                                                                                     AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCA  >  VH01111:79:AACJV3JHV:2:2606:50441:32029/1‑153 (MQ=60)

                                                                                                                     AGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGCCTGCCCCAACTGCATCCGCCCCAGGAGGCTTTCATACCCCTCCGGCCCCGGTGCA  >  VH01111:79:AACJV3JHV:2:2606:50706:31613/1‑153 (MQ=60)

GAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCA  >  AE015451_phaM1/6011032‑6011301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`