Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F109 I1 R1	11	69.2	2403364	94.6%	2273582	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,305	C→T	R395C (CGT→TGT)	icd →	isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,305	0	C	T	97.6%	228.9 / NA	85	R395C (CGT→TGT)	icd	isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (49/32); minor base A (2/0); total (52/33)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTT  >  NC_000913/1196165‑1196444

aGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGGGCAATCACCGCGAGAACCGTAACCTATGACTTCGAgtgt                                                                                                                                           >  1:217312/1‑143 (MQ=255)

aGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAgtgt                                                                                                                                           <  1:400726/143‑1 (MQ=255)

 ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTc                                                                                                                                          <  2:541445/143‑1 (MQ=255)

 ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTc                                                                                                                                          >  1:178219/1‑143 (MQ=255)

  gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCGACGCGAAAACCGTAACCTATGACTTCGAGTGTCt                                                                                                                                         >  1:604732/1‑143 (MQ=255)

  gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCt                                                                                                                                         >  1:239830/1‑143 (MQ=255)

    cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAGGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTga                                                                                                                                       <  1:532403/143‑1 (MQ=255)

    cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGCGTGTCTga                                                                                                                                       >  1:918742/1‑143 (MQ=255)

    cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTga                                                                                                                                       <  1:1116239/143‑1 (MQ=255)

    cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTga                                                                                                                                       <  1:662384/143‑1 (MQ=255)

    cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTga                                                                                                                                       <  1:476677/143‑1 (MQ=255)

     aaaGTAAATCCTGGCTCTATCATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCCACGCAAAAACCGTAACCTATGACTTCGAGTGTCTgat                                                                                                                                      <  1:1200909/143‑1 (MQ=255)

       aGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatgg                                                                                                                                    >  1:719247/1‑143 (MQ=255)

        gTAAATCCTGGCTCTATTATTCTCTCCGCTGCGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCCATCACTCCGACCGTCCgctgga                                                                                                                                   >  2:80211/1‑143 (MQ=25)

        gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatgga                                                                                                                                   >  1:987429/1‑143 (MQ=255)

             tCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgcg                                                                                                                              >  1:395277/1‑143 (MQ=255)

              ccTGGCTCTATTATTCGCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgcgc                                                                                                                             <  1:196965/143‑1 (MQ=255)

                tGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGGCATCAACGCGGAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTa                                                                                                                           >  1:1188170/1‑143 (MQ=255)

                    tctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTACCCTATGACTTCGAGTATCTGATGGATGGCGCTAAAct                                                                                                                       >  2:614047/1‑143 (MQ=255)

                    tctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTACGACTTCGAGAGTCTGATGGAGGGCGCTAAAct                                                                                                                       >  2:466557/1‑143 (MQ=255)

                     ctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAATGTATGGAAGGCGCAATCAACGCGAGAACCGTAACCTATAACTTCGAGTGTCTGATGGATGGCGCTAAActg                                                                                                                      <  1:172707/143‑1 (MQ=255)

                       attattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAActgct                                                                                                                    >  2:127941/1‑143 (MQ=255)

                       attattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAActgct                                                                                                                    >  1:284288/1‑143 (MQ=255)

                        ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAActgctg                                                                                                                   <  1:987228/143‑1 (MQ=255)

                        ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAActgatg                                                                                                                   >  1:696315/1‑143 (MQ=255)

                             ctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATg                                                                                                              >  1:57509/1‑143 (MQ=255)

                               ctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTCAACTGCTGAAATGtt                                                                                                            >  1:185260/1‑143 (MQ=255)

                               ctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCCAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGtt                                                                                                            >  2:330467/1‑143 (MQ=255)

                                   gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAACTTAAATAGCGTTTGGCTGATGGATGACGCTAAACTGCTGAAATATTCaga                                                                                                        >  2:711644/1‑143 (MQ=18)

                                     tGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGACGGCGCAAACACCGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGt                                                                                                      >  1:857554/1‑143 (MQ=255)

                                       agaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAGAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt                                                                                                    >  1:759887/1‑143 (MQ=255)

                                       agaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt                                                                                                    >  1:95073/1‑143 (MQ=255)

                                         atgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTgg                                                                                                  >  1:812153/1‑143 (MQ=255)

                                          tgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt                                                                                                 <  1:484921/143‑1 (MQ=255)

                                           gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGGTTGGTg                                                                                                >  2:367903/1‑143 (MQ=255)

                                           gatgCTGCGCCACATGGGTAGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCCGAAATGTTCAGCGTTTGGTg                                                                                                >  2:1130227/1‑143 (MQ=255)

                                            acgctgcGCCACATGGGTTGGACCGAAGCGACTTACTTAATGGTTAAAGGTATGGGAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa                                                                                               <  1:810241/141‑1 (MQ=18)

                                              gctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACGTCGAGAGTCTGATCGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg                                                                                             >  2:1038026/1‑143 (MQ=255)

                                                tgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGCGTTTGGTGAcgcg                                                                                           >  2:333725/1‑143 (MQ=255)

                                                 gcgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGCCGGGTCGAGACTGCTGAAATGTTCAGAGTTTGGTGACGCGa                                                                                          >  2:526139/1‑143 (MQ=18)

                                                        aTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGACGGTGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGCTTGGTGACGCGATCATCGa                                                                                   >  2:772194/1‑143 (MQ=255)

                                                         tGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaa                                                                                  <  1:888016/143‑1 (MQ=255)

                                                             ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCACTCAACGCGAAAACCGTAACCTATGACTTCGGGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGCTTGGTGACGCGAGCATCGCGAACa                                                                              >  2:834309/1‑143 (MQ=18)

                                                              ttgACCGAAGCCGCTGGCTTAATTGGTAAAGGGATGGCAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACAt                                                                             <  2:407163/141‑1 (MQ=18)

                                                                 aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTa                                                                          >  2:936865/1‑143 (MQ=255)

                                                                  ccGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTaa                                                                         >  2:278186/1‑143 (MQ=255)

                                                                      aGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGcc                                                                     <  1:125557/143‑1 (MQ=255)

                                                                            tGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt                                                               >  2:1151778/1‑143 (MQ=255)

                                                                            tGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt                                                               >  2:126613/1‑143 (MQ=255)

                                                                                ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACCTGTAATGCCGTCGCTTGtt                                                           >  1:262490/1‑143 (MQ=255)

                                                                                ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTACTGCCGTAGTTTGtt                                                           >  1:947917/1‑143 (MQ=255)

                                                                                ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGtt                                                           >  1:458123/1‑143 (MQ=255)

                                                                                  aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAACTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTaa                                                         >  1:1187311/1‑143 (MQ=255)

                                                                                  aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGACAACAGTTAATGACGTAGTTTGTTac                                                         >  2:432390/1‑142 (MQ=18)

                                                                                   aTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTaaa                                                        >  1:350990/1‑143 (MQ=255)

                                                                                     tGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt                                                      <  1:770367/143‑1 (MQ=255)

                                                                                       gtAAAGGTATGGTAGGCGCAATCAACGAGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta                                                    <  2:1035039/142‑1 (MQ=255)

                                                                                         aaaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGGTTGTTagagctatt                                                  >  2:726359/1‑135 (MQ=255)

                                                                                             gTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACg                                              <  1:828294/143‑1 (MQ=255)

                                                                                             gTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGAGGGCGCTCCACAAACAAAATGTTCAGAGTTTGGAGACCCGATCACCGAAAACATGTAATGCCGTCGCTTGTTACATTTATTAACg                                              >  2:1044614/1‑143 (MQ=18)

                                                                                                gtgAAGGCGCAATCAACGCGTAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGa                                           <  2:696315/141‑1 (MQ=255)

                                                                                                  gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGc                                         <  2:178219/143‑1 (MQ=255)

                                                                                                  gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGGTTGTTAAATTTATTAACGGGGGc                                         >  2:545414/1‑143 (MQ=255)

                                                                                                         cAATCAGCGCGAGAACCGTCACCTTTGACTTGGGGGGTCAGAAGAATGGCGCTAAACTGCTGAAGTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGc                                  <  2:526213/143‑1 (MQ=25)

                                                                                                          aaTCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGGGCGTAACGcg                                 >  1:380005/1‑142 (MQ=255)

                                                                                                          aaTCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCt                                 >  1:703656/1‑143 (MQ=255)

                                                                                                             cAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACCTGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTccc                              <  2:759887/143‑1 (MQ=255)

                                                                                                                cgcgAAAACCGTAACCTATGACGTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgtt                           <  2:807194/143‑1 (MQ=255)

                                                                                                                  cgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTTACGCTCCCgttaa                         <  2:918742/143‑3 (MQ=255)

                                                                                                                  cgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgttgt                         >  1:89030/1‑143 (MQ=255)

                                                                                                                    aaagCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACGGCGGAAATGTTCAGAGTTTGGCGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCGCCCGTTGttt                       <  2:633637/143‑1 (MQ=25)

                                                                                                                     aaaCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttt                      >  1:296785/1‑143 (MQ=255)

                                                                                                                      aaCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCAGTTGttttt                     >  1:994766/1‑143 (MQ=255)

                                                                                                                       aCCGTATCCTATGACTTCGAGTGTTTGATTGATGGCGCTAAACGGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAGATTTATTAACGGGAGCGTAACGCTCCCGTTGtttttt                    <  1:569965/143‑1 (MQ=25)

                                                                                                                       aCCGTAACCGATGACTTCGAGTGTGTGATGGCTGTCTCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATAGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTACCGGGAGCGTAACGCTCCCGTTGtttttt                    <  1:755676/143‑1 (MQ=25)

                                                                                                                        ccGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTg                   >  2:971927/1‑143 (MQ=255)

                                                                                                                         cGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGATTTCTCCATAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGt                  <  1:871453/143‑1 (MQ=37)

                                                                                                                         cGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGt                  >  2:319902/1‑143 (MQ=255)

                                                                                                                          gTAACCTATGAGTTCTGGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAACCATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTTACGCTCCCgttaataaattta                 <  2:376403/143‑11 (MQ=25)

                                                                                                                             aCCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGCGATACGACACTCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAgg              <  1:1190418/143‑1 (MQ=37)

                                                                                                                             aCCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAgg              <  2:395277/143‑1 (MQ=255)

                                                                                                                               cTATGACTTCGAGTGTCTGATGGATGGCAATAAACAGCTGAAATGTTCAGAGTTTCGTGACGCGATCATCGAACACATGTAATGCCGTAGTTTGTTAATTTTATTCACGGGAGAGTACAGCCCACGTTGCTTTTTGATAGgct            >  2:325940/1‑143 (MQ=21)

                                                                                                                                tATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGAGGCGTAGCGCTCCCGTTGTTTTTTGTTAGgctg           >  1:39002/1‑143 (MQ=255)

                                                                                                                                 cggACTTGGGGTGTCTGGTGGATGGCGCTAAACTGCGTAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAAGGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCCCCCGTTGTTTTTTGTTAGgctgc          <  2:1187311/141‑1 (MQ=37)

                                                                                                                                    gcTTCGAGTGGCTGATGGATGGCGCGAAACTGCTGAAAATTTTTGAGTTTTGTGAAGAGAGCATGAAGAGCATGTAATGCCGTAGTTTGTTAATTTTATTATCGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTaa       <  1:747028/142‑1 (MQ=255)

                                                                                                                                      ttCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACg     >  1:725425/1‑143 (MQ=255)

                                                                                                                                         gAGTGTCTGATGGATGGCGCTATACGGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGtt  <  1:969301/143‑1 (MQ=255)

AGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTT  >  NC_000913/1196165‑1196444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A