Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F106 I0 R1	88	30.3	670850	93.2%	625232	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	584,121	G→T	100%	intergenic (+205/‑67)	rplA → / → rplJ	ribosomal protein L1/ribosomal protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	584,121	0	G	T	100.0%	120.3 / NA	38	intergenic (+205/‑67)	rplA/rplJ	ribosomal protein L1/ribosomal protein L10
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (22/16); total (22/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTC  >  CP000730/583983‑584252

aGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTGATTTCTAAGTTAAAATTTATCCTGCCGAGGCTGAAATTGACTGGAACGTGATGCTCTATGATCTTTCAACCACTTATTGCCTTg                                                                                                                                   >  1:187998/1‑141 (MQ=255)

              ttccgatcTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTGTTTTTAACTTAATATTTATCCTGCCGAGGCTGAAATTGACTTGGACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCtt                                                                                                                     <  2:29256/134‑1 (MQ=255)

                      ttATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTttatt                                                                                                             <  2:253167/141‑1 (MQ=255)

                      ttATTGGAATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCATGCCGAGGATAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTtataat                                                                                                             <  2:253119/141‑3 (MQ=255)

                             gATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTTATTTTa                                                                                                      >  2:312165/1‑141 (MQ=255)

                             gATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTa                                                                                                      <  1:154699/141‑1 (MQ=255)

                              attattTTACCTAGGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGAACTTTTTGCCTTGGGTGGAAAGTGCTTTTTTTATTAATTTTaa                                                                                                     >  2:181930/1‑141 (MQ=255)

                                 attTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTaaagatcggaa                                                                                                  >  1:29256/1‑132 (MQ=255)

                                   tttACCTAAGACAGTAGGAGTTATTTCTAACTTAAAATCTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaaaaa                                                                                                <  1:203711/141‑1 (MQ=255)

                                     tACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGc                                                                                              >  1:281873/1‑141 (MQ=255)

                                         tAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCa                                                                                          >  1:334343/1‑141 (MQ=255)

                                            gACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCaaaa                                                                                       >  1:56858/1‑141 (MQ=255)

                                                    gAGTTATTTATAACTTAACATCTATCCTGCCGAGGCTAAAATTGAATTGAACGTGATGATCTAGGATCTTTCAAGCACTTTTTGCCTTGGGTAGGAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCACAAAGTTaaag                                                                               >  2:152280/1‑140 (MQ=255)

                                                    gAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAAt                                                                               >  1:286332/1‑141 (MQ=255)

                                                     aGTTATTCAGAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATg                                                                              <  2:126239/141‑1 (MQ=255)

                                                      gTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATgg                                                                             <  1:226884/141‑1 (MQ=255)

                                                           ttatAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGgtg                                                                        >  2:320502/1‑141 (MQ=255)

                                                           ttatAACTTAAAATTTAGCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGgtg                                                                        <  2:253440/141‑1 (MQ=255)

                                                                          tATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTa                                                         >  2:313800/1‑141 (MQ=255)

                                                                           aTCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTAt                                                        >  1:82969/1‑141 (MQ=255)

                                                                                  cGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGAGTCTGAATGTCTGCTATCATTGaa                                                 >  1:106573/1‑141 (MQ=255)

                                                                                     ggCTAAACTTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGGGTCTGAATGTCTTCTATCATGGAAGca                                              >  2:121661/1‑140 (MQ=255)

                                                                                     ggCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATGATGTTTAAAAAAAGCACCAAAAATTTAATTAGAGGTGTCTGAATCCCTGCTATCATTGAAGCt                                              >  1:328739/1‑141 (MQ=255)

                                                                                      gCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACTGTGAAGTTTAATGGAGGTGTCTGTATGTCTGCTATCGTTGGAGCTa                                             >  1:257845/1‑141 (MQ=255)

                                                                                         aaaaTTGACTTGAAAGTGATGGTCTATGATCTTTCAAGTACTTTTTGGCTTTGGTAGAAAGGGCTGTTGTCATTAATTCTAAAACAGGAACGATTAAGGTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTaaag                                          <  2:152401/141‑2 (MQ=255)

                                                                                             ttGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAaaca                                      <  2:136022/141‑1 (MQ=255)

                                                                                               gACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTAATAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAaacaac                                    <  2:162555/141‑1 (MQ=255)

                                                                                                  ttGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAg                                 >  2:213449/1‑141 (MQ=255)

                                                                                                      aCGTGATGATCTATGATCTTTCAAGCACTTTTGGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTtga                             <  1:3967/141‑1 (MQ=255)

                                                                                                         tgatgaTCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATGTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTtgatga                          <  1:295657/141‑1 (MQ=255)

                                                                                                                ttatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCAATGAAGATAAAAAACAACTAGTTGATGAAATTGCt                   <  2:112954/140‑1 (MQ=255)

                                                                                                                  atgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGa                 <  1:313800/141‑1 (MQ=255)

                                                                                                                      tctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTa             >  2:277439/1‑141 (MQ=255)

                                                                                                                             aGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTGAAAAACAACTAGTTGATGAAATTGCGGAGGTAGTATCaa      >  1:320407/1‑141 (MQ=255)

                                                                                                                             aGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCaa      >  1:125501/1‑141 (MQ=255)

                                                                                                                               cACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATAGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAAt    >  2:213878/1‑141 (MQ=255)

                                                                                                                                aCTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAAtt   <  1:312165/141‑1 (MQ=255)

                                                                                                                                 cTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTc  >  1:1150/1‑141 (MQ=255)

AGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTC  >  CP000730/583983‑584252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A