Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F58 I1 R2	17	42.8	915862	63.3%	579740	150.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	584,121	G→T	intergenic (+205/‑67)	rplA → / → rplJ	ribosomal protein L1/ribosomal protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	584,121	0	G	T	100.0%	80.6 / NA	26	intergenic (+205/‑67)	rplA/rplJ	ribosomal protein L1/ribosomal protein L10
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (19/7); total (19/7)

CAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTT  >  CP000730/583978‑584257

cAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACGTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGTCTATAATTGACTTTAACGTGATGATCTATGATCTTTCAAGCACTTTTTTCCTTGGGTAg                                                                                                                                   >  2:261070/1‑151 (MQ=255)

  aTAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGaa                                                                                                                                 >  2:207592/1‑151 (MQ=255)

     aGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGAAGGATTTATTTATAACTTAAAATTTATCCTGTCGAGGCTAAAATTGACATAAACGTGATAATCTATGATCTTTCAAGCACTTTTTACCTTGGGTAGAAAGt                                                                                                                              >  2:57419/1‑151 (MQ=255)

          tttCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCttt                                                                                                                         >  1:77805/1‑151 (MQ=255)

                             aTTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaaaa                                                                                                      >  2:87185/1‑151 (MQ=255)

                             aTTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaaaa                                                                                                      >  2:87134/1‑151 (MQ=255)

                                          tACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAAtt                                                                                         >  1:77292/1‑151 (MQ=255)

                                             cTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTaa                                                                                      >  2:49124/1‑151 (MQ=255)

                                              tAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTaaa                                                                                     >  1:288048/1‑151 (MQ=255)

                                                aGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGAAAAAAAAATTTAAATg                                                                                   <  2:127016/151‑1 (MQ=255)

                                                                       ttAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATc                                                            <  2:77805/151‑1 (MQ=255)

                                                                       ttAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATc                                                            >  2:454208/1‑151 (MQ=255)

                                                                              ttATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAg                                                     >  1:210194/1‑151 (MQ=255)

                                                                                       cGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAaac                                            >  1:78098/1‑151 (MQ=255)

                                                                                        gAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAaaca                                           <  1:87134/151‑1 (MQ=255)

                                                                                        gAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAaaca                                           <  1:87185/151‑1 (MQ=255)

                                                                                                    gACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAATAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTtgatga                               <  1:48710/151‑1 (MQ=255)

                                                                                                           aCGTGATGATCTATGATCTTTCAAGCACTTTTTTTTTTTGTTTGAAAGAGCGTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGTAGTCTGAATGTCTGCTATAATTGAAGTTAAAAAACAACTAGTTGTTGTAATTGCt                        >  2:139192/1‑151 (MQ=255)

                                                                                                           aCGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTTTTTTTTTTTAAAAAAGCACCAAAAATTTAAATGTAGTTTTCTGGATGTCTGCTCTCATTGTTGCTCTAACACAATTAGTTGATGAAATTGCt                        >  2:235636/1‑151 (MQ=255)

                                                                                                              tgatgaTCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTTATTTTAAAAAAAGCACAAAAAATATAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAg                     >  2:395522/1‑151 (MQ=255)

                                                                                                              tgatgaTCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAg                     <  1:257171/151‑1 (MQ=255)

                                                                                                                  gatctatgatctTTCAAGCACTTTTTTTTTTTTTTTGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTCCTGAGGTAc                 >  2:173598/1‑151 (MQ=255)

                                                                                                                        tgatctTTCAAGCACTTTTTGCTTTGGGTAGAAAGTGCTTTTTTTTTTTATTTTTAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTTAATGTCTGCTATCATTTAAGCTAAAAATCAACTATTTGTTGAAATTGCTTAGGTACTATCaa           >  2:176236/1‑151 (MQ=255)

                                                                                                                          atctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAAt         <  1:280849/151‑1 (MQ=255)

                                                                                                                               tCAAGCACTTGTGGCCTTTGGTAGAAAGTGCTTTTTTTGTTGATTTTAAAAAAAGCACCAAAAATTTAAATGGATGTGTCTGAATGTCTGCTATCATTGATGCTAAAAAACAACTAGTTGATGAAATTGCTGAGATACTATCAAATTCaat    >  2:86112/1‑149 (MQ=255)

                                                                                                                                 aaGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTCTAAAAAAAGCACCAAAAATTTCACTGTAGGTGTCTGAATCTCTGCTATCATTCAAGCTAAAAACCAACTACTTGATGAAATTGCTCAGGTACTATCAACTTCAGttt  >  2:339499/1‑151 (MQ=255)

CAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTT  >  CP000730/583978‑584257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A