Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F999 I0 R1
|
96 |
52.5 |
3964927 |
95.8% |
3798400 |
49.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,720,597 |
Δ1 bp |
coding (4/153 nt) |
hokA ← |
small toxic polypeptide |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,720,596 | 0 | G | . | 100.0%
| 226.3
/ NA
| 46 | coding (5/153 nt) | hokA | small toxic polypeptide |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (24/22); total (24/22) |
TAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCA > NC_000913/3720547‑3720641
|
tAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCg < 1:3405494/50‑1 (MQ=255)
tAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCg < 1:1522797/50‑1 (MQ=255)
aaaaGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCg > 1:3181180/1‑49 (MQ=255)
aaaaGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCg > 1:1967643/1‑49 (MQ=255)
aaaGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCgca < 1:641889/50‑3 (MQ=255)
cGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTcc < 1:1062017/50‑1 (MQ=255)
cGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTcc < 1:3216334/50‑1 (MQ=255)
cGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTcc > 1:29198/1‑50 (MQ=255)
tGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa < 1:673082/50‑1 (MQ=255)
tGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa < 1:3119292/50‑1 (MQ=255)
tGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa < 1:2124565/50‑1 (MQ=255)
gAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAg > 1:2857058/1‑50 (MQ=255)
aaaCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAg < 1:3068779/49‑1 (MQ=255)
aaCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcc > 1:3452137/1‑50 (MQ=255)
aCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcct < 1:1548254/50‑1 (MQ=255)
cAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctc > 1:2509953/1‑50 (MQ=255)
cAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctc > 1:2652369/1‑50 (MQ=255)
aTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtg < 1:136918/50‑1 (MQ=255)
cTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt > 1:76872/1‑50 (MQ=255)
tAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt > 1:2945166/1‑50 (MQ=255)
aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt > 1:2138333/1‑50 (MQ=255)
aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt > 1:2250612/1‑50 (MQ=255)
aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt < 1:1160235/49‑1 (MQ=255)
aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc < 1:1911206/50‑1 (MQ=255)
agaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc < 1:3127669/50‑1 (MQ=255)
agaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc > 1:1990255/1‑49 (MQ=255)
gaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc > 1:1736739/1‑49 (MQ=255)
gaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCa < 1:3695487/50‑1 (MQ=255)
aaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCaa > 1:1306146/1‑50 (MQ=255)
aagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc < 1:246700/50‑1 (MQ=255)
aagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc < 1:1088658/50‑1 (MQ=255)
aagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc > 1:3067325/1‑50 (MQ=255)
aagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc < 1:3156294/50‑1 (MQ=255)
agTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc > 1:3075851/1‑49 (MQ=255)
gTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc > 1:1702175/1‑48 (MQ=255)
tAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg < 1:1372543/50‑1 (MQ=255)
tAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg < 1:2033890/50‑1 (MQ=255)
tAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg > 1:605453/1‑50 (MQ=255)
aaTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg > 1:8697/1‑49 (MQ=255)
aaTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg > 1:1057148/1‑49 (MQ=255)
aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGt < 1:974501/49‑1 (MQ=255)
aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTa < 1:2888253/50‑1 (MQ=255)
cTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTAt > 1:2457989/1‑49 (MQ=255)
tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaa > 1:2547688‑M1/1‑48 (MQ=255)
tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaa > 1:806603‑M1/1‑48 (MQ=255)
atatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaac > 1:1530556‑M1/1‑47 (MQ=255)
tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATa < 1:116640‑M1/47‑2 (MQ=255)
atTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactt > 1:953512‑M1/1‑45 (MQ=255)
tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactt < 1:836130‑M1/49‑6 (MQ=255)
tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttga > 1:3515911‑M1/1‑42 (MQ=255)
tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttg < 1:3176127‑M1/49‑8 (MQ=255)
|
TAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCA > NC_000913/3720547‑3720641
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
