Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A21 F999 I0 R1
|
108 |
30.5 |
2257647 |
98.2% |
2217009 |
49.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,720,597 |
Δ1 bp |
coding (4/153 nt) |
hokA ← |
small toxic polypeptide |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,720,596 | 0 | G | . | 100.0%
| 202.4
/ NA
| 42 | coding (5/153 nt) | hokA | small toxic polypeptide |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (14/28); total (14/28) |
AAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTG > NC_000913/3720549‑3720639
|
aaaGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCgca > 1:2180301/1‑48 (MQ=255)
aaaGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCgc < 1:1946593/49‑2 (MQ=255)
gCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc < 1:1907158/50‑1 (MQ=255)
gCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc < 1:614710/50‑1 (MQ=255)
gCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc < 1:346598/50‑1 (MQ=255)
gTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTcc > 1:1096412/1‑49 (MQ=39)
tGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa < 1:2068742/50‑1 (MQ=255)
tGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa < 1:441513/50‑1 (MQ=255)
aaaCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAg < 1:2200185/49‑1 (MQ=255)
aaaCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAg < 1:349460/49‑1 (MQ=255)
aaCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcc > 1:339217/1‑50 (MQ=255)
aaTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctcct > 1:1893311/1‑50 (MQ=255)
aTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtg > 1:2227819/1‑50 (MQ=255)
aCTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTgtgt < 1:2091893/50‑1 (MQ=255)
aCTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTgtgt < 1:1152617/50‑1 (MQ=255)
aCTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTgtgt > 1:8742/1‑50 (MQ=255)
cTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt < 1:672938/50‑1 (MQ=255)
cTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt < 1:1130791/50‑1 (MQ=255)
aTTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGa > 1:1621554/1‑50 (MQ=255)
aTTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGa > 1:1458581/1‑50 (MQ=255)
ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGa < 1:1918847/49‑1 (MQ=255)
ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGa < 1:1144175/49‑1 (MQ=255)
tAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt > 1:1304171/1‑50 (MQ=255)
aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt < 1:774781/49‑1 (MQ=255)
aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt < 1:9254/49‑1 (MQ=255)
aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt < 1:2040848/49‑1 (MQ=255)
aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt < 1:2204146/49‑1 (MQ=255)
aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt < 1:600365/49‑1 (MQ=255)
aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt < 1:1420432/49‑1 (MQ=255)
gaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCa < 1:109944/50‑1 (MQ=255)
gaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCa < 1:1161514/50‑1 (MQ=255)
aaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCaa > 1:2235997/1‑50 (MQ=255)
agTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACg < 1:1222590/50‑1 (MQ=255)
agTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACg < 1:597867/50‑1 (MQ=255)
gTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGa < 1:1598267/50‑1 (MQ=255)
cTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTAt > 1:139160/1‑49 (MQ=255)
cTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTAt > 1:229986/1‑49 (MQ=255)
cTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTAt > 1:1832980/1‑49 (MQ=255)
tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTGTaact > 1:2102394‑M1/1‑46 (MQ=255)
tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTAt < 1:1641853/46‑1 (MQ=255)
tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaact > 1:1046644‑M1/1‑46 (MQ=255)
tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATa < 1:1827738‑M1/47‑2 (MQ=255)
tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaacttt < 1:2230046‑M1/50‑7 (MQ=255)
tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaacttt < 1:1202222‑M1/50‑7 (MQ=255)
|
AAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTG > NC_000913/3720549‑3720639
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
