Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I0 R1 26 138.6 6843716 94.5% 6467311 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,754,639 C→A 100% Q42H (CAG→CAT)  ratA ← toxic UPF0083 family protein inhibitor of 70S ribosome formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,754,6390CA99.4% 551.6 / ‑5.2 157Q42H (CAG→CAT) ratAtoxic UPF0083 family protein inhibitor of 70S ribosome formation
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (84/72);  total (84/73)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 161 total aligned reads displayed.

ACGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTC  >  NC_000913/2754542‑2754734
                                                                                                 |                                                                                               
aCGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATg                                                                                               >  2:2814121/1‑100 (MQ=255)
aCGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATg                                                                                               >  2:1782997/1‑100 (MQ=255)
 cGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGa                                                                                              <  2:1179641/100‑1 (MQ=255)
  gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                             <  2:3204474/100‑1 (MQ=255)
  gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                             <  2:683107/100‑1 (MQ=255)
  cttccgatcttCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                             <  2:1702692/90‑1 (MQ=255)
    tttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGa                                                                                           <  2:3327426/100‑1 (MQ=255)
    tttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGa                                                                                           <  1:1030987/100‑1 (MQ=255)
       gCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATaa                                                                                        >  2:2433346/1‑100 (MQ=255)
       gCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATaa                                                                                        >  2:1958191/1‑100 (MQ=255)
       gCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATaa                                                                                        <  2:1868886/100‑1 (MQ=255)
          gATCCCGGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                     <  1:2811493/100‑1 (MQ=255)
          gATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                     >  1:2276195/1‑100 (MQ=255)
           aTCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACt                                                                                    >  2:2681722/1‑100 (MQ=255)
           aTCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGACCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACt                                                                                    >  1:2104648/1‑100 (MQ=255)
            tCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAgagatcggaag                                                                                   >  1:1702684/1‑90 (MQ=255)
             cccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTAAAAAAAAAAAAAAAATGAGGATAAGACTGa                                                                                  <  1:2212547/100‑1 (MQ=255)
               cAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGATACAACCCGGCAAAAAATGAGGATAAGACTGAAc                                                                                <  1:46399/100‑1 (MQ=255)
                aGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACg                                                                               <  2:830134/100‑1 (MQ=255)
                 tccTTATAGACATCTACCGCCGCAGTAATCTGCCCATGAGTGGAAGCCAGAATCCGACTTCCGGTACAACCCTGCAAAAAATGAGGATAAGACTGAACg                                                                               <  1:866494/98‑1 (MQ=255)
                 gCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGt                                                                              >  1:1038099/1‑100 (MQ=255)
                  ccTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTc                                                                             <  1:582490/100‑1 (MQ=255)
                   cTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCa                                                                            >  1:1266416/1‑100 (MQ=255)
                     tAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAtt                                                                          >  2:469845/1‑100 (MQ=255)
                      agagACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAtt                                                                          <  2:2696009/99‑1 (MQ=255)
                      agagACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAAAAAAGAAAAAAATGAGGATAAGACTGAACGTCATTc                                                                         <  1:3212203/100‑1 (MQ=255)
                         gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                      <  2:3224108/100‑1 (MQ=255)
                           cATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTaa                                                                    >  2:29122/1‑100 (MQ=255)
                             tCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                                  <  2:1264675/100‑1 (MQ=255)
                             tCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                                  >  2:3013386/1‑100 (MQ=255)
                             tCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                                  <  2:2743446/100‑1 (MQ=255)
                               tACCGCCGCAGTCATCTGCACAGGAGTTGAGGTTAGATGTCGTCATCCAGTAAAACAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAAc                                                                   <  1:671838/97‑1 (MQ=255)
                                aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTg                                                                 <  1:2797397/98‑1 (MQ=255)
                                 ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCCAAAAATGAGGATAAGACTGAACGTCCTTCACTAACTGATa                                                              >  2:1873802/1‑100 (MQ=255)
                                 ccgacgCCGTAATCTGCCCAGGAGTCGACTCCAGAATCCGACTTCAGGTACAACCCGGCAAAAAATGAGGATACGACTGAACGTCATTCACTAACTGATa                                                              <  1:2211123/100‑1 (MQ=255)
                                   gccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACa                                                            <  2:1102439/100‑1 (MQ=255)
                                    ccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAt                                                           >  2:1079530/1‑100 (MQ=255)
                                     cgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAtt                                                          <  2:1428656/100‑1 (MQ=255)
                                      gcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAttt                                                         <  2:2910071/100‑1 (MQ=255)
                                       cAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCTGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAttt                                                         <  1:1563500/99‑1 (MQ=255)
                                       cAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTg                                                        >  1:3111576/1‑100 (MQ=255)
                                           cATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTcc                                                    >  1:1355957/1‑100 (MQ=255)
                                           cATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTcc                                                    >  1:485734/1‑100 (MQ=255)
                                           cATCTGCCCAGGAGTGGACTCCAGAATCCAACTTCCGATACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCt                                                      <  1:696574/98‑1 (MQ=255)
                                              cTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCcgcg                                                 <  2:2640829/100‑1 (MQ=255)
                                               tGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTAAAAAACGACAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCcgcg                                                 <  1:1358700/99‑1 (MQ=255)
                                                gCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                               >  1:2747514/1‑100 (MQ=255)
                                                gCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                               >  1:171712/1‑100 (MQ=255)
                                                 cccAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                               >  1:1062470/1‑99 (MQ=255)
                                                   cAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTa                                            >  2:2416045/1‑100 (MQ=255)
                                                    aGGAGTGGACTCCAGAATCCGACTTCCGGTACAACACAACAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                               <  1:2106616/96‑1 (MQ=255)
                                                     ggAGTGCACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                          <  1:1498091/100‑1 (MQ=255)
                                                      gAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAggg                                         <  1:1785091/100‑1 (MQ=255)
                                                          ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                     <  1:3126627/100‑1 (MQ=255)
                                                          ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                     >  2:3346911/1‑100 (MQ=255)
                                                          ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                     <  1:1111141/100‑1 (MQ=255)
                                                          ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAc                                      <  1:1983345/99‑1 (MQ=255)
                                                          ggACTCAAGAATCCGACTTCCGGTACAACCGGGCAAAAAAAGAGGATAAGACTGAACGTCATTAACTAACTGATACATTTGCTGCGAGCTGTAGGGTAc                                      >  1:753380/1‑99 (MQ=255)
                                                            aCTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAg                                   <  2:2789572/100‑1 (MQ=255)
                                                             cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                                  >  2:2550054/1‑100 (MQ=255)
                                                               ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                >  2:2506993/1‑100 (MQ=255)
                                                               ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                <  1:358629/100‑1 (MQ=255)
                                                               ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                <  2:1462687/100‑1 (MQ=255)
                                                                 aGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCTCTAACTGATACATTTGCTCCGCGCTGTATGGTACCAGTGccg                              <  2:2314557/100‑3 (MQ=255)
                                                                 aGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                              <  2:3036146/100‑1 (MQ=255)
                                                                  gAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGt                             <  1:2494674/100‑1 (MQ=255)
                                                                   aaTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATACGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACAAGTGCGGTc                            >  1:1697725/1‑100 (MQ=255)
                                                                   aaTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCGCCGCGCTGTAGGGTACCAGTGCGGTc                            <  1:2143671/100‑1 (MQ=255)
                                                                     tCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCg                          <  2:2141601/100‑1 (MQ=255)
                                                                     tCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCg                          <  1:2537860/100‑1 (MQ=255)
                                                                       cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                        >  2:2045781/1‑100 (MQ=255)
                                                                       cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                        >  2:3095662/1‑100 (MQ=255)
                                                                       cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                        >  2:1026644/1‑100 (MQ=255)
                                                                        gACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCt                       >  1:522495/1‑100 (MQ=255)
                                                                        gACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCt                       >  2:775911/1‑100 (MQ=255)
                                                                        gACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCt                       <  1:2686947/100‑1 (MQ=255)
                                                                        gACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCt                       <  1:839012/100‑1 (MQ=255)
                                                                          cTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTaa                     >  1:2637490/1‑100 (MQ=255)
                                                                          cTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTaa                     >  2:3392646/1‑100 (MQ=255)
                                                                           ttCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAAt                    >  2:3140036/1‑100 (MQ=255)
                                                                            tCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATc                   >  1:306412/1‑100 (MQ=255)
                                                                             ccGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCt                  >  1:2011241/1‑100 (MQ=255)
                                                                             ccGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCt                  >  2:1017525/1‑100 (MQ=255)
                                                                               ggTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGa                <  1:774457/100‑1 (MQ=255)
                                                                               ggTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGa                <  2:1594164/100‑1 (MQ=255)
                                                                                gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg               <  1:645668/100‑1 (MQ=255)
                                                                                 tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg              <  1:2351227/100‑1 (MQ=255)
                                                                                   cAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCa            >  2:2366060/1‑100 (MQ=255)
                                                                                     aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa          >  1:3256997/1‑100 (MQ=255)
                                                                                     aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa          >  2:2325279/1‑100 (MQ=255)
                                                                                      cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa         >  2:887387/1‑100 (MQ=255)
                                                                                      cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa         >  2:1110422/1‑100 (MQ=255)
                                                                                       ccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAAc        >  2:1757593/1‑100 (MQ=255)
                                                                                         aaaaaaaaaTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACa       <  1:1961713/96‑1 (MQ=255)
                                                                                           cAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAtt    >  2:1559558/1‑100 (MQ=255)
                                                                                           cAAAAAAAGAGAATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAGCAGTGCGGTCCGGCTAATCTGAGGCATAACAAtt    >  1:1800233/1‑100 (MQ=255)
                                                                                            aaaaaaTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAttt   >  2:1432120/1‑100 (MQ=255)
                                                                                             aaaaATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTc  <  1:906824/100‑1 (MQ=255)
                                                                                             aaaaATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTc  >  1:40853/1‑100 (MQ=255)
                                                                                             aaaaATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTc  >  1:2350998/1‑100 (MQ=255)
                                                                                                 |                                                                                               
ACGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTC  >  NC_000913/2754542‑2754734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A