Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 14 141.9 7085095 93.3% 6610393 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,754,639 C→A Q42H (CAG→CAT)  ratA ← toxic UPF0083 family protein inhibitor of 70S ribosome formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,754,6390CA99.2% 463.0 / ‑3.3 131Q42H (CAG→CAT) ratAtoxic UPF0083 family protein inhibitor of 70S ribosome formation
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (65/65);  total (65/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 133 total aligned reads displayed.

CGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCC  >  NC_000913/2754543‑2754735
                                                                                                |                                                                                                
cGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGa                                                                                               >  2:3523753/1‑100 (MQ=255)
 gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                              <  2:1755725/100‑1 (MQ=255)
 gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                              <  2:3540818/100‑1 (MQ=255)
 gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                              <  1:970177/100‑1 (MQ=255)
 gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                              <  2:1213623/100‑1 (MQ=255)
 gTTTTGCGGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                              <  2:3304799/100‑1 (MQ=255)
   tttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGa                                                                                            >  1:3487684/1‑100 (MQ=255)
    ttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGAt                                                                                           >  2:910089/1‑100 (MQ=255)
    ttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGACAAAAAATGAGGAt                                                                                           <  2:2738496/100‑1 (MQ=255)
     tGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATa                                                                                          <  2:3122520/100‑1 (MQ=255)
     tGCTGATCCCAGCCTTAGAGACATATACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATa                                                                                          >  2:1765227/1‑100 (MQ=255)
            cccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGGATCCGACTTCCGGTAAAAAAAGAAAAAAAATGAGGATAAGACTGa                                                                                   <  1:1443192/100‑1 (MQ=255)
             ccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGaa                                                                                  <  2:2025929/100‑1 (MQ=255)
              cAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAAc                                                                                 <  2:793386/100‑1 (MQ=255)
               aGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGAAAAAAAAAAAAAAAAATGAGGATAAGACTGAACg                                                                                <  1:2668283/100‑1 (MQ=255)
                gCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGt                                                                               >  1:2037143/1‑100 (MQ=255)
                     agagACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTc                                                                          <  1:479608/100‑1 (MQ=255)
                     agagACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTc                                                                          >  1:2611843/1‑100 (MQ=255)
                     agagACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTc                                                                          <  1:1648007/100‑1 (MQ=255)
                     agagACATCCACCGCCGAAGTCATCTGCCCAGGAGAAGACTCCAGAATCCGACTTCCGGAAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTc                                                                          <  1:1157379/100‑1 (MQ=255)
                      gagaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCGGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCa                                                                         >  2:2387570/1‑100 (MQ=255)
                      gagaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCa                                                                         >  1:2424933/1‑100 (MQ=255)
                       agaCATCTACCGCCGGAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCAc                                                                        <  2:3487791/100‑1 (MQ=255)
                       agaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAAcagatcggaa                                                                        >  1:1699230/1‑91 (MQ=255)
                        gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                       <  1:1814793/100‑1 (MQ=255)
                        gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                       <  1:3050026/100‑1 (MQ=255)
                         aCATCTACCGCCGCAGTCATATGACCAATAAAAGACTCCAGAATGCGACTTCCTGTAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTa                                                                      <  1:841641/100‑1 (MQ=255)
                          cATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTaa                                                                     >  1:705796/1‑100 (MQ=255)
                          cATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTaa                                                                     >  1:708921/1‑100 (MQ=255)
                            tCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                                   >  1:2782829/1‑100 (MQ=255)
                            tCTACCGACGGTGTTATCTGCGTAGGAGTGAACTCCATTATCCGATTCCCGGTACATCCCCGCAATAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                                   <  1:3417468/100‑1 (MQ=255)
                            cttACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAAACAGGAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                                   <  1:2806164/98‑1 (MQ=255)
                             cTACCGCCGAAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTAAAAAACAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTg                                                                  <  1:1154714/100‑1 (MQ=255)
                              tACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACtag                                                                 >  2:2806184/1‑98 (MQ=255)
                              tACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGa                                                                 >  2:1820449/1‑100 (MQ=255)
                               aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGAt                                                                <  2:1277350/100‑1 (MQ=255)
                                ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                               >  2:2877978/1‑100 (MQ=255)
                                ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                               >  2:2540057/1‑100 (MQ=255)
                                ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                               >  2:2877059/1‑100 (MQ=255)
                                 cgccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGAt                                                                <  1:826920/98‑1 (MQ=255)
                                 cgccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATAc                                                              <  1:2372613/100‑1 (MQ=255)
                                    cgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAtt                                                           <  2:660528/100‑1 (MQ=255)
                                     gcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAttt                                                          >  1:2591967/1‑100 (MQ=255)
                                     gcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACAAAGAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAttt                                                          <  1:661717/100‑1 (MQ=255)
                                      cAGTCATCTGCCCAGGAGTGGACTCCAGACTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATCAGACTGAACGTCATTCACTAACTGATACATTTg                                                         <  1:2132988/100‑1 (MQ=255)
                                       aGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGc                                                        <  2:3010110/100‑1 (MQ=255)
                                          cATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTcc                                                     >  1:1828677/1‑100 (MQ=255)
                                          cATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTcc                                                     >  1:958093/1‑100 (MQ=255)
                                          cATCTGCCCAGGAGTGGACTCCAGAAGCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTcc                                                     <  1:1220948/100‑1 (MQ=255)
                                           ntCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCcg                                                    >  2:1110738/2‑100 (MQ=255)
                                             cTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTTCTCcgcg                                                  <  2:1862998/100‑1 (MQ=255)
                                               gCCCAGGCGTGCACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAGCGTCATTCACTAACTGATACATTTGCTCcgcgag                                                >  1:1862987/1‑98 (MQ=255)
                                                  cAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTa                                             >  2:9698/1‑100 (MQ=255)
                                                  cAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCACTggt                                             >  1:1565669/1‑98 (MQ=255)
                                                   acaagtaaaCTCCAGACTGCGACTGCCGGGACAAAAAAGAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAg                                            <  1:3404726/92‑1 (MQ=255)
                                                    ggAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                           <  2:4109/100‑1 (MQ=255)
                                                       gTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTa                                        <  1:2716828/100‑1 (MQ=255)
                                                        tGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAc                                       <  2:1828688/100‑1 (MQ=255)
                                                         ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                      >  1:32309/1‑100 (MQ=255)
                                                         ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                      <  2:839549/100‑1 (MQ=255)
                                                            cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGCTACATTTGCTCCGCGCTGTAGGGTACCAGt                                   >  2:1678527/1‑100 (MQ=255)
                                                              ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                 >  1:1078484/1‑100 (MQ=255)
                                                              ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                 >  1:395245/1‑100 (MQ=255)
                                                              ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                 >  1:1078739/1‑100 (MQ=255)
                                                                aGAATCCGACTTCCGGTACAACCCGGCAAAAAATGGGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                               <  1:1024924/100‑1 (MQ=255)
                                                                aGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                               <  2:488986/100‑1 (MQ=255)
                                                                aGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                               <  2:201115/100‑1 (MQ=255)
                                                                 gAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGt                              >  1:1719540/1‑100 (MQ=255)
                                                                    tacGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTcc                            >  1:1750961/3‑99 (MQ=255)
                                                                     ccGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCgg                          >  2:82700/1‑100 (MQ=255)
                                                                      cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                         >  2:1780550/1‑100 (MQ=255)
                                                                      cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                         <  1:2407160/100‑1 (MQ=255)
                                                                      cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                         >  2:2542212/1‑100 (MQ=255)
                                                                      cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                         >  2:1318843/1‑100 (MQ=255)
                                                                       gACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCt                        <  1:983963/100‑1 (MQ=255)
                                                                       atcTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTACGGCt                        <  2:3468903/98‑1 (MQ=255)
                                                                        aCTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTa                       >  1:37427/1‑100 (MQ=255)
                                                                        aCTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTa                       <  2:708924/100‑1 (MQ=255)
                                                                          ttCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAAt                     >  2:1903541/1‑100 (MQ=255)
                                                                           tCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATc                    >  2:2211617/1‑100 (MQ=255)
                                                                           tCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTACGGCTagat                    >  1:3468876/1‑97 (MQ=255)
                                                                             cGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTg                  >  1:435231/1‑100 (MQ=255)
                                                                               gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg                >  2:2769948/1‑100 (MQ=255)
                                                                               gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg                >  1:1302479/1‑100 (MQ=255)
                                                                                tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg               <  2:2620964/100‑1 (MQ=255)
                                                                                tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg               <  2:271335/100‑1 (MQ=255)
                                                                                 aCAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc              >  1:3141368/1‑100 (MQ=255)
                                                                                 aCAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc              >  1:1431039/1‑100 (MQ=255)
                                                                                 aCAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc              <  2:2037156/100‑1 (MQ=255)
                                                                                  cAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCa             <  2:16762/100‑1 (MQ=255)
                                                                                   aaCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCAt            >  2:2549962/1‑100 (MQ=255)
                                                                                    aaccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa           <  1:2109696/98‑1 (MQ=255)
                                                                                    aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa           >  1:2997004/1‑100 (MQ=255)
                                                                                    aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa           >  2:2958553/1‑100 (MQ=255)
                                                                                    aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa           >  1:1079813/1‑100 (MQ=255)
                                                                                     cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGATCCGGCTAATCTGAGGCATaa          >  1:1517903/1‑100 (MQ=255)
                                                                                       cGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACa        <  2:966262/100‑1 (MQ=255)
                                                                                          cAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAtt     >  2:377507/1‑100 (MQ=255)
                                                                                           aaaaaaTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAttt    >  2:2648386/1‑100 (MQ=255)
                                                                                             aaaaTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTcc  >  2:1508481/1‑100 (MQ=255)
                                                                                                |                                                                                                
CGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCC  >  NC_000913/2754543‑2754735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A