Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F120 I0 R1 38 148.3 7410925 93.3% 6914393 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,754,639 C→A 100% Q42H (CAG→CAT)  ratA ← toxic UPF0083 family protein inhibitor of 70S ribosome formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,754,6390CA99.3% 519.9 / ‑3.8 149Q42H (CAG→CAT) ratAtoxic UPF0083 family protein inhibitor of 70S ribosome formation
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (72/75);  total (74/75)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

ACGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTC  >  NC_000913/2754542‑2754734
                                                                                                 |                                                                                               
aCGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATg                                                                                               <  1:1055442/100‑1 (MQ=255)
aCGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATg                                                                                               <  1:20585/100‑1 (MQ=255)
 cGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGa                                                                                              <  2:2384706/100‑1 (MQ=255)
  gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                             <  2:582050/100‑1 (MQ=255)
  gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                             >  2:222444/1‑100 (MQ=255)
  gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGGCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                             <  2:3235374/100‑1 (MQ=255)
   ttttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTACAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                             <  1:2572010/99‑1 (MQ=255)
    tttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGa                                                                                           >  2:3036233/1‑100 (MQ=255)
    tttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGa                                                                                           >  1:1514472/1‑100 (MQ=255)
     ttGCTGATCCCCGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCACGAGTGGACTCCATAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGa                                                                                           <  1:2098497/99‑1 (MQ=255)
      tGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATa                                                                                         <  1:1581913/100‑1 (MQ=255)
        cTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAg                                                                                       <  2:923028/100‑1 (MQ=255)
        cTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAg                                                                                       >  1:2457298/1‑100 (MQ=255)
         tGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGa                                                                                      >  2:454184/1‑100 (MQ=255)
           aTCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACt                                                                                    >  2:2011030/1‑100 (MQ=255)
           aTCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGAAGTGGACTCCAGAATCCTACTTCCGGTACAACACAACAAAAAATGAGGATAAGACt                                                                                    <  1:76869/100‑1 (MQ=255)
           aTCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCACAGGAGTGGACTCCAGAATCCGACTTCAGGTACAATCCGGCAAAAAATGAGGATAAGACt                                                                                    <  1:74761/100‑1 (MQ=255)
             cccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGa                                                                                  >  2:2256180/1‑100 (MQ=255)
               cAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAAc                                                                                <  2:1354893/100‑1 (MQ=255)
                aGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACg                                                                               >  1:602206/1‑100 (MQ=255)
                   cTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACgcca                                                                            >  2:1164750/1‑97 (MQ=255)
                   cTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCa                                                                            >  2:2226968/1‑100 (MQ=255)
                    ttAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAt                                                                           <  2:1502800/100‑1 (MQ=255)
                       tccgatcttACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCa                                                                        <  1:2011042/92‑1 (MQ=255)
                         gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                      <  2:2745930/100‑1 (MQ=255)
                         gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                      >  1:2168377/1‑100 (MQ=255)
                         gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTAAAAAAAAACAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                      <  1:1642634/100‑1 (MQ=255)
                         gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACACCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                      >  1:1978993/1‑100 (MQ=255)
                          aCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTa                                                                     <  2:2019477/100‑1 (MQ=255)
                           cATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGACTCCGACTTCCGGTACAACCAGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTaa                                                                    >  1:2015121/1‑100 (MQ=255)
                           cATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTaa                                                                    >  2:2125822/1‑100 (MQ=255)
                              cTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTg                                                                 >  1:2512469/1‑100 (MQ=255)
                              cTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTg                                                                 <  1:3541000/100‑1 (MQ=255)
                               tACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGa                                                                >  1:2479262/1‑100 (MQ=255)
                                aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGAt                                                               <  2:241414/100‑1 (MQ=255)
                                aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGAt                                                               <  1:1619011/100‑1 (MQ=255)
                                aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATCAGGATAAGACTGAACGTCATTCACTAACTGAt                                                               <  2:3510356/100‑1 (MQ=255)
                                aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCAAGAATCCGACTTCCGGTACAACCCGTCAAAAAATGAGGATAAGACTGAACGTGATTCACTAACTGAt                                                               >  1:3265301/1‑100 (MQ=255)
                                 ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                              >  1:3159130/1‑100 (MQ=255)
                                 ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                              >  1:1687267/1‑100 (MQ=255)
                                 ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                              >  2:2778487/1‑100 (MQ=255)
                                 ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                              <  2:2351151/100‑1 (MQ=255)
                                  cgccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATAc                                                             >  2:2969984/1‑100 (MQ=255)
                                  cgccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATAc                                                             >  2:1461705/1‑100 (MQ=255)
                                     cgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAtt                                                          >  1:2826509/1‑100 (MQ=255)
                                     cgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAtt                                                          <  2:1083278/100‑1 (MQ=255)
                                        aGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGc                                                       <  2:2021920/100‑1 (MQ=255)
                                        aGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGc                                                       <  2:2106464/100‑1 (MQ=255)
                                        aGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGc                                                       >  2:2097051/1‑100 (MQ=255)
                                          tCATCTGCAAAAAAGTAGACCCCAGAATCCGACTTCCGGTAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTc                                                     <  1:1832501/100‑1 (MQ=255)
                                           cATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTcc                                                    >  2:1632146/1‑100 (MQ=255)
                                           cATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTcc                                                    >  2:2251258/1‑100 (MQ=255)
                                            aTCTGACCAGCAGTGCACTCCAGAACCCGCCTCCCGGTACAACTCCACAAAAAATGACAATAAGCCTCAACCTCATTCACTCACTAATACATTTGCTCcg                                                   <  1:2206160/100‑1 (MQ=255)
                                              cTGCCCAGGAGTGGCCTCCAGTATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATACGACTGAACGTCTTTCACTAACTGATACATTGGCTCcgc                                                  >  1:1462549/1‑99 (MQ=255)
                                              cTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCcgcg                                                 >  1:2821700/1‑100 (MQ=255)
                                              cTGCCCAAAAGTAGACTCCAGAATCCTACTTCCGGTAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTg                                                        <  1:1026152/93‑1 (MQ=255)
                                               tGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGATGATAAGTCTGAACGTCATTCACTAACTGATACATTTGCTCCgcgc                                                <  1:1442503/100‑1 (MQ=255)
                                                gCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                               <  1:2609028/100‑1 (MQ=255)
                                                gCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                               >  2:2895372/1‑100 (MQ=255)
                                                  ccAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGt                                             <  2:3116205/100‑1 (MQ=255)
                                                  ccAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGt                                             >  1:1018493/1‑100 (MQ=255)
                                                  ccAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGt                                             <  2:2817630/100‑1 (MQ=255)
                                                   cAGGAGTGGACTCCAGACTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTa                                            <  2:2585397/100‑1 (MQ=255)
                                                     ggAGTAGACTCCAGAATCCGACTTCCTGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                          <  2:444331/100‑1 (MQ=255)
                                                      gAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAggg                                         >  2:60659/1‑100 (MQ=255)
                                                       aGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGt                                        <  1:1082095/100‑1 (MQ=255)
                                                         tGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTatgcaac                                      >  1:1944527/1‑94 (MQ=255)
                                                          ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                     <  2:2652109/100‑1 (MQ=255)
                                                          ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                     <  1:2272928/100‑1 (MQ=255)
                                                          ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                     <  2:619971/100‑1 (MQ=255)
                                                           gACTCCAGAAGCCGACTTCCGGTACAAACCGGAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAc                                      <  1:2349137/98‑1 (MQ=255)
                                                            aCTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAg                                   <  2:1027082/100‑1 (MQ=255)
                                                            aCTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAg                                   <  2:2087386/100‑1 (MQ=255)
                                                            aCTCCAGAATCCGACTTCCGGAAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAg                                   <  1:266717/100‑1 (MQ=255)
                                                             cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                                  <  2:2575585/100‑1 (MQ=255)
                                                               ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                >  2:3188081/1‑100 (MQ=255)
                                                                cAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCg                               <  1:2989127/100‑1 (MQ=255)
                                                                 aGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                              >  2:2329775/1‑100 (MQ=255)
                                                                 aGAATCCGACTTCCGGTACAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                <  1:3413882/98‑1 (MQ=255)
                                                                  gAATCCGACTTCCGGTACAACCAGGCAAAAAATGACAATAATACTGAACATCACTCACTAACTGATACATTTGCACCGCGCAGTAGGGTACCAGTGCg                               >  1:632165/1‑98 (MQ=255)
                                                                   aaTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTc                            <  1:2718514/100‑1 (MQ=255)
                                                                     tCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCg                          >  2:2662370/1‑100 (MQ=255)
                                                                       cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                        <  2:932744/100‑1 (MQ=255)
                                                                        gACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCt                       <  2:1554685/100‑1 (MQ=255)
                                                                           ttCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAAt                    >  1:3702420/1‑100 (MQ=255)
                                                                           ttCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAAt                    >  1:3459328/1‑100 (MQ=255)
                                                                                gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg               <  1:476260/100‑1 (MQ=255)
                                                                                gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg               <  1:970351/100‑1 (MQ=255)
                                                                                gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg               <  2:1612128/100‑1 (MQ=255)
                                                                                  aCAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc             >  2:64380/1‑100 (MQ=255)
                                                                                   cAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCa            >  1:303553/1‑100 (MQ=255)
                                                                                     aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa          >  1:3365667/1‑100 (MQ=255)
                                                                                     aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa          >  2:282125/1‑100 (MQ=255)
                                                                                      cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa         <  1:996905/100‑1 (MQ=255)
                                                                                      cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa         >  1:2685618/1‑100 (MQ=255)
                                                                                          gCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAt     <  2:3474556/100‑1 (MQ=255)
                                                                                           cAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAtt    >  2:1896672/1‑100 (MQ=255)
                                                                                            aaaaaaTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAttt   <  1:3558348/100‑1 (MQ=255)
                                                                                             aaaaATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTc  <  2:1758045/100‑1 (MQ=255)
                                                                                             aaaaATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGACATAACAATTTc  <  1:3202712/100‑1 (MQ=255)
                                                                                                 |                                                                                               
ACGTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTC  >  NC_000913/2754542‑2754734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A