Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F69 I1 R1 15 111.3 5655542 93.7% 5299242 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,754,639 C→A Q42H (CAG→CAT)  ratA ← toxic UPF0083 family protein inhibitor of 70S ribosome formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,754,6390CA99.2% 429.8 / ‑3.3 120Q42H (CAG→CAT) ratAtoxic UPF0083 family protein inhibitor of 70S ribosome formation
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (57/62);  total (58/62)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 122 total aligned reads displayed.

GTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCC  >  NC_000913/2754544‑2754735
                                                                                               |                                                                                                
 ttttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAgg                                                                                             >  2:882485/1‑100 (MQ=255)
 ttttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAgg                                                                                             <  1:1408069/100‑1 (MQ=255)
  tttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGa                                                                                            >  1:377989/1‑100 (MQ=255)
      cTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATaa                                                                                         >  2:2284474/1‑99 (MQ=255)
      cTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAg                                                                                        >  2:1927196/1‑100 (MQ=255)
        gATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                      <  1:102773/100‑1 (MQ=255)
         aTCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACt                                                                                     <  1:678875/100‑1 (MQ=255)
         aTCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACt                                                                                     <  1:1841389/100‑1 (MQ=255)
          tCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTg                                                                                    >  1:807930/1‑100 (MQ=255)
           cctaGCCTTAGAGACATTTACGGTCGCAGTCATCTGCGCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGAAAAAAATGAGGATAAGACt                                                                                     >  2:2671259/4‑98 (MQ=255)
           cccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGa                                                                                   <  1:1343241/100‑1 (MQ=255)
           cccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGa                                                                                   <  1:1342794/100‑1 (MQ=255)
           cccAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGa                                                                                   >  2:875983/1‑100 (MQ=255)
             cAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGATTGGACTCCAGAATCCGACTTCCGGTACAAAAAAGAAAAAAATGAGGATAAGACTGAAc                                                                                 <  2:2741262/100‑1 (MQ=255)
             cAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAAc                                                                                 >  1:2006982/1‑100 (MQ=255)
              aGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACg                                                                                >  2:1065686/1‑100 (MQ=255)
              aGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACg                                                                                <  2:559834/100‑1 (MQ=255)
                 cTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCa                                                                             >  1:1138096/1‑100 (MQ=255)
                  ttAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAt                                                                            <  1:681678/100‑1 (MQ=255)
                   tAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCAt                                                                            <  2:1836081/99‑1 (MQ=255)
                   atctgaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAtt                                                                           <  2:1665535/96‑1 (MQ=255)
                    agagACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTc                                                                          <  2:2693474/100‑1 (MQ=255)
                    agagACATCCACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAAGCCATCTTCTGGTAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTc                                                                          <  2:878048/100‑1 (MQ=255)
                     gagaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCa                                                                         <  2:2823114/100‑1 (MQ=255)
                     gagaCATCAACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGAAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTc                                                                          <  2:1417521/99‑1 (MQ=255)
                      agaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCAc                                                                        <  1:875986/100‑1 (MQ=255)
                       tctaTCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                       <  2:780630/97‑1 (MQ=255)
                       gaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAttagat                                                                       >  1:1665541/1‑96 (MQ=255)
                        aCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTa                                                                      <  1:2093397/100‑1 (MQ=255)
                        aCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAAACCAGCAAAAAATGAGGATAAGACTGAACGTCATTCACTa                                                                      <  1:2362303/100‑1 (MQ=255)
                        aCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCAAAAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                       <  2:2283244/99‑1 (MQ=255)
                         cATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGATTCCGACTTCCGGTACAACCCGGCAAAAAATGATGATAAGACTGAACGTCAtt                                                                           <  2:2417350/94‑1 (MQ=255)
                          aTCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTaga                                                                    >  1:780633/1‑98 (MQ=255)
                           tCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                                   <  1:2286752/100‑1 (MQ=255)
                           tCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                                   >  1:1384211/1‑100 (MQ=255)
                             tACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGa                                                                 >  2:1406582/1‑100 (MQ=255)
                             tACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGa                                                                 >  2:574557/1‑100 (MQ=255)
                              aCCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACAGAt                                                                >  2:1606774/1‑100 (MQ=255)
                               ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                               >  1:2604318/1‑100 (MQ=255)
                                   cgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAtt                                                           >  2:1844645/1‑100 (MQ=255)
                                     cAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTg                                                         <  1:161875/100‑1 (MQ=255)
                                     cAGTCATCTGCCCAAGAGTAGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTg                                                         <  2:2499674/100‑1 (MQ=255)
                                      aGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGc                                                        <  1:28582/100‑1 (MQ=255)
                                       gTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCt                                                       <  1:811499/100‑1 (MQ=255)
                                         cATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTcc                                                     >  1:2070771/1‑100 (MQ=255)
                                           tCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCcgc                                                   >  2:1521780/1‑100 (MQ=255)
                                              gCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCgcgc                                                 <  1:2360875/99‑1 (MQ=255)
                                              gCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                                >  1:1842848/1‑100 (MQ=255)
                                               cccAGGAGTGTACTCCAGAATCCGACTTCCGGTACAACCCCTCAAAAAATGAGGCTACGACTGAACGTCATTCACTCCCTGATACATTTGCTCCGCGCt                                                <  2:1884966/99‑1 (MQ=255)
                                                 cAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTa                                             <  1:731193/100‑1 (MQ=255)
                                                  aGGAGTGGACTCCAGAAGCCGACTTCCGGTAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAg                                            <  2:1444257/100‑1 (MQ=255)
                                                   ggAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                           <  1:2029372/100‑1 (MQ=255)
                                                   ggAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                           >  2:1770904/1‑100 (MQ=255)
                                                   ggAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                           >  2:1172308/1‑100 (MQ=255)
                                                      gTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTa                                        <  1:1770913/100‑1 (MQ=255)
                                                      gTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTa                                        <  2:1662731/100‑1 (MQ=255)
                                                      gTGGACTCCAGAATCCGACTTCCGGAAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGt                                         <  2:117198/99‑1 (MQ=255)
                                                       tGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAc                                       <  1:2299156/100‑1 (MQ=255)
                                                       tGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAc                                       >  2:1016978/1‑100 (MQ=255)
                                                        ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                      <  2:125946/100‑1 (MQ=255)
                                                        ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                      <  2:1138092/100‑1 (MQ=255)
                                                        gatcTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                      <  2:449201/97‑1 (MQ=255)
                                                           cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAACATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCa                                     >  2:2825837/1‑98 (MQ=255)
                                                           cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                                   >  2:2225999/1‑100 (MQ=255)
                                                           cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                                   >  1:805028/1‑100 (MQ=255)
                                                             ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAgatc                                 >  1:449204/1‑97 (MQ=255)
                                                             ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                                   >  2:1863211/1‑98 (MQ=255)
                                                             ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                 >  2:2222035/1‑100 (MQ=255)
                                                             ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                 <  1:2057621/100‑1 (MQ=255)
                                                              cAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCg                                >  1:757723/1‑100 (MQ=255)
                                                               aGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                               <  1:342192/100‑1 (MQ=255)
                                                               aGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                               <  1:1759741/100‑1 (MQ=255)
                                                               aGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                               <  2:1384205/100‑1 (MQ=255)
                                                                gAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCgg                               <  1:1021298/99‑1 (MQ=255)
                                                                  aTCCGACTTCCGGTACAACCCGGTAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTcc                            <  2:858207/100‑1 (MQ=255)
                                                                  aTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTcc                            >  2:1278787/1‑100 (MQ=255)
                                                                    ccGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCgg                          <  1:262462/100‑1 (MQ=255)
                                                                    ccGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCgg                          <  1:1016982/100‑1 (MQ=255)
                                                                    ccGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCgg                          >  2:1676827/1‑100 (MQ=255)
                                                                     cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                         >  1:2239371/1‑100 (MQ=255)
                                                                      gACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCt                        <  2:1757632/100‑1 (MQ=255)
                                                                        cTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTaa                      <  2:398758/100‑1 (MQ=255)
                                                                        cTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTaa                      >  1:71413/1‑100 (MQ=255)
                                                                          tCCGGTACAACCCGGGAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATc                    <  1:2780864/100‑1 (MQ=255)
                                                                           ccGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCt                   >  2:2351973/1‑100 (MQ=255)
                                                                           ccGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCt                   >  2:2470278/1‑100 (MQ=255)
                                                                            cGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTg                  >  1:2466701/1‑100 (MQ=255)
                                                                              gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg                <  1:1676833/100‑1 (MQ=255)
                                                                                aCAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc              <  1:1049291/100‑1 (MQ=255)
                                                                                 cAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCa             >  2:1180967/1‑100 (MQ=255)
                                                                                   aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa           >  2:2809769/1‑100 (MQ=255)
                                                                                   aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa           >  2:2166370/1‑100 (MQ=255)
                                                                                   aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa           >  2:2396541/1‑100 (MQ=255)
                                                                                    cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGTGTACCCGTACAGTCCGGCTAATCTGCGGCATaa          >  2:2599282/1‑100 (MQ=255)
                                                                                    cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa          >  1:309306/1‑100 (MQ=255)
                                                                                         cAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAAtt     >  1:1617508/1‑100 (MQ=255)
                                                                                          aaaaaaTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACa        <  2:2796987/96‑1 (MQ=255)
                                                                                           aaaaATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTc   <  2:2751715/100‑1 (MQ=255)
                                                                                           aaaaATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTc   >  1:1556528/1‑100 (MQ=255)
                                                                                            aaaaTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTcc  >  2:2006158/1‑100 (MQ=255)
                                                                                               |                                                                                                
GTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCC  >  NC_000913/2754544‑2754735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A