Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F113 I1 R1 18 155.0 7745081 93.4% 7233905 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,754,639 C→A Q42H (CAG→CAT)  ratA ← toxic UPF0083 family protein inhibitor of 70S ribosome formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,754,6390CA98.0% 520.3 / ‑0.5 148Q42H (CAG→CAT) ratAtoxic UPF0083 family protein inhibitor of 70S ribosome formation
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (76/69);  total (78/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

GTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCC  >  NC_000913/2754544‑2754735
                                                                                               |                                                                                                
gTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                              <  1:2753205/100‑1 (MQ=255)
gTTGTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGGCTTCCGGTACAGCCCGGCAAAAAATGAg                                                                                              <  2:3269573/100‑1 (MQ=255)
 ttttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAgg                                                                                             <  2:1055569/100‑1 (MQ=255)
 ttttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAg                                                                                              <  1:2759176/99‑1 (MQ=255)
  tttGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGa                                                                                            <  1:3546566/100‑1 (MQ=255)
    tGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATa                                                                                          <  2:1309664/100‑1 (MQ=255)
    tGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATa                                                                                          >  2:1341086/1‑100 (MQ=255)
     gCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATaa                                                                                         >  1:880328/1‑100 (MQ=255)
     gCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATaa                                                                                         >  1:2221259/1‑100 (MQ=255)
      cTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAg                                                                                        >  2:148965/1‑100 (MQ=255)
       tGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGa                                                                                       >  1:2282870/1‑100 (MQ=255)
        gATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                      >  2:2314313/1‑100 (MQ=255)
        gATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                      >  2:2511842/1‑100 (MQ=255)
         aTCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACt                                                                                     >  1:812424/1‑100 (MQ=255)
          tCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTg                                                                                    >  2:128738/1‑100 (MQ=255)
          tCCCAGCCTTAGAGACATCTACCGCCGCAGGCATCTGCACAGGAGTGGTCCCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGAc                                                                                      <  1:3123861/98‑1 (MQ=255)
           cccAGCCTTAGAGCCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGa                                                                                   >  1:2599635/1‑100 (MQ=255)
             cAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAAc                                                                                 <  1:711453/100‑1 (MQ=255)
             cAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAAc                                                                                 >  1:85158/1‑100 (MQ=255)
             cAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAAc                                                                                 <  2:678807/100‑1 (MQ=255)
              aGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACg                                                                                <  2:161189/100‑1 (MQ=255)
                ccTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTc                                                                              <  1:1364849/100‑1 (MQ=255)
                 cTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCa                                                                             <  1:3819260/100‑1 (MQ=255)
                   tAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAtt                                                                           <  2:1516791/100‑1 (MQ=255)
                   tAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCAtt                                                                           >  1:3766584/1‑100 (MQ=255)
                     gagaCATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCa                                                                         >  2:1220720/1‑100 (MQ=255)
                        tcATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTAAAAACAAGCAAAAAATGAGGATAAGACTGAACGTCATTCACt                                                                       <  2:911973/98‑1 (MQ=255)
                         cATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTag                                                                     >  1:911975/1‑99 (MQ=255)
                         cATCTACCGCCGCAGGCATCTGCCCAGGAGTGGACGCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAAGGGGGATAAGACGGAACGGCATGCAc                                                                        >  1:1141605/1‑97 (MQ=255)
                          aTCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAAc                                                                    >  1:2773076/1‑100 (MQ=255)
                          aTCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAAc                                                                    >  2:1586748/1‑100 (MQ=255)
                            cTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTg                                                                  >  2:2767786/1‑100 (MQ=255)
                             tACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGa                                                                 >  1:2864372/1‑100 (MQ=255)
                               ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                               >  2:1050667/1‑100 (MQ=255)
                               ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                               >  2:2058178/1‑100 (MQ=255)
                               ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                               >  1:3761101/1‑100 (MQ=255)
                               ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                               >  1:2704744/1‑100 (MQ=255)
                               ccgccgCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATa                                                               >  1:1363094/1‑100 (MQ=255)
                                cgccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATAc                                                              >  1:2185404/1‑100 (MQ=255)
                                cgccgcAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATAc                                                              >  1:513595/1‑100 (MQ=255)
                                 gccgaAATCATATGCCCAGGAATAGACTCTAGAATCCCAATTCCTGTAAAAAAAAAAAAAAAATGAGGATAAGACTGAACGTCATTCACTAACt                                                                   <  2:802840/94‑1 (MQ=255)
                                     cAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACAttt                                                          >  2:3868157/1‑99 (MQ=255)
                                           tCTGCCCAGGAGTGGACTCCAGGATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCcgc                                                   >  1:1350559/1‑100 (MQ=255)
                                            cTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCcgcg                                                  <  1:1398494/100‑1 (MQ=255)
                                             tGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCgcgc                                                 >  2:2163479/1‑100 (MQ=255)
                                              gCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCt                                                >  1:2671667/1‑100 (MQ=255)
                                                ccAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGt                                              >  1:2432977/1‑100 (MQ=255)
                                                 cAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTa                                             >  1:2676173/1‑100 (MQ=255)
                                                  aGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAg                                            <  1:550263/100‑1 (MQ=255)
                                                   ggAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGTTATATTTGCTCCGCGCTGTAgg                                           <  2:392184/100‑1 (MQ=255)
                                                   ggAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAgg                                           <  2:3811602/100‑1 (MQ=255)
                                                    gAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAggg                                          >  2:603008/1‑100 (MQ=255)
                                                     aGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGACATTCACTAACTGATACATTTGCTTCGCGCTGTAGGGt                                         <  2:2388158/100‑1 (MQ=255)
                                                      gTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTa                                        <  2:85015/100‑1 (MQ=255)
                                                        ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                      >  2:3318972/1‑100 (MQ=255)
                                                        ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                      <  2:569577/100‑1 (MQ=255)
                                                        ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                      <  1:1532740/100‑1 (MQ=255)
                                                        ggACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTAcc                                      <  1:309639/100‑1 (MQ=255)
                                                         gACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCa                                     <  2:748367/100‑1 (MQ=255)
                                                          aCTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAg                                    <  1:3336136/100‑1 (MQ=255)
                                                          aCTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAg                                    <  2:701943/100‑1 (MQ=255)
                                                          aCTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAg                                    >  2:2910418/1‑100 (MQ=255)
                                                           cTCCAGAATCCGACTTCCGGTACAACGCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCGGTAGGGTACCAGt                                   >  2:3222335/1‑100 (MQ=255)
                                                           cTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGt                                   >  2:2743018/1‑100 (MQ=255)
                                                             ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                 >  1:1182858/1‑100 (MQ=255)
                                                             ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                 <  1:399875/100‑1 (MQ=255)
                                                             ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                 <  2:2664024/100‑1 (MQ=255)
                                                             ccAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGc                                 >  2:1750047/1‑100 (MQ=255)
                                                              cAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCg                                >  1:1970096/1‑100 (MQ=255)
                                                              cAGAATCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCg                                <  1:673761/100‑1 (MQ=255)
                                                                  aTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTcc                            >  1:3607351/1‑100 (MQ=255)
                                                                  aTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTcc                            >  1:1650559/1‑100 (MQ=255)
                                                                  aTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTcc                            <  2:1955642/100‑1 (MQ=255)
                                                                  aTCCGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTcc                            >  1:2109199/1‑100 (MQ=255)
                                                                     cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                         >  1:2368302/1‑100 (MQ=255)
                                                                     cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                         <  2:1696385/100‑1 (MQ=255)
                                                                     cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                         >  2:522410/1‑100 (MQ=255)
                                                                     cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                         >  1:342312/1‑100 (MQ=255)
                                                                     cGACTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGc                         <  2:1350556/100‑1 (MQ=255)
                                                                       aCTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTa                       <  2:2091782/100‑1 (MQ=255)
                                                                        cTTCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTaa                      <  1:128739/100‑1 (MQ=255)
                                                                         ttCCGGTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAAt                     >  2:1217834/1‑100 (MQ=255)
                                                                             ggTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGa                 <  2:2984141/100‑1 (MQ=255)
                                                                              gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg                <  1:397411/100‑1 (MQ=255)
                                                                              gTACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAg                <  1:1427886/100‑1 (MQ=255)
                                                                               tACAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgg               <  2:3039065/100‑1 (MQ=255)
                                                                                aCAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGc              >  2:2276127/1‑100 (MQ=255)
                                                                                 cAACCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCa             <  1:148966/100‑1 (MQ=255)
                                                                                  aaCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCAt            >  2:634111/1‑100 (MQ=255)
                                                                                  aaCCCGGCAAAAAATGAGGATAAGACTGAACGGCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCAt            <  2:3609096/100‑1 (MQ=255)
                                                                                   aCCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATa           <  2:2671652/100‑1 (MQ=255)
                                                                                   aCCCGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAgatcgg           >  1:1820796/1‑95 (MQ=255)
                                                                                    cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa          >  2:397750/1‑100 (MQ=255)
                                                                                    cccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATaa          >  2:398006/1‑100 (MQ=255)
                                                                                     ccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAAc         <  2:2746835/100‑1 (MQ=255)
                                                                                     ccGGCAAAAAATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAAc         <  1:3516550/100‑1 (MQ=255)
                                                                                           aaaaATGAGTATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTc   >  2:3471287/1‑100 (MQ=255)
                                                                                           aaaaATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTc   <  1:1586752/100‑1 (MQ=255)
                                                                                           aaaaATGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTc   <  1:1926030/100‑1 (MQ=255)
                                                                                            aaaaTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTcc  >  1:2494927/1‑100 (MQ=255)
                                                                                               |                                                                                                
GTTTTGCTGATCCCAGCCTTAGAGACATCTACCGCCGCAGTCATCTGCCCAGGAGTGGACTCCAGAATCCGACTTCCGGTACAACCCGGCAAAAACTGAGGATAAGACTGAACGTCATTCACTAACTGATACATTTGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCC  >  NC_000913/2754544‑2754735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A