Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F129 I1 R1
|
16 |
78.2 |
4038333 |
91.8% |
3707189 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,917,345 |
G→A |
G763G (GGG→GGA) |
barA → |
hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,917,345 | 0 | G | A | 95.2%
| 205.5
/ 0.4
| 63 | G763G (GGG→GGA) | barA | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (25/35); total (27/36) |
| Fisher's exact test for biased strand distribution p-value = 5.72e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 |
CTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCG > NC_000913/2917250‑2917439
|
cTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGa > 1:757859/1‑100 (MQ=255)
tCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAg < 1:824684/100‑1 (MQ=255)
tCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAg > 1:296033/1‑100 (MQ=255)
aTCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCg > 1:876522/1‑100 (MQ=255)
ccaccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAGAGAGAAGCTGCTTGGCGCAGGAATGAGCGAt > 2:1360342/1‑100 (MQ=255)
caccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGAtt > 2:1988863/1‑100 (MQ=255)
accaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTa < 2:33758/100‑1 (MQ=255)
cTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCTCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTgg < 1:831657/100‑1 (MQ=255)
cccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCg > 1:1214108/1‑100 (MQ=255)
cccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCg > 1:1757362/1‑100 (MQ=255)
ccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGa > 2:479673/1‑100 (MQ=255)
aTCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAAcc < 1:798865/100‑1 (MQ=255)
cAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCg > 2:411154/1‑99 (MQ=255)
cAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGa > 2:1772092/1‑100 (MQ=255)
aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAt > 2:20809/1‑100 (MQ=255)
aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAt > 1:1390758/1‑100 (MQ=255)
caacaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTg < 2:296034/100‑1 (MQ=255)
caacaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTg < 2:708655/100‑1 (MQ=255)
caacaaACGCCGGTTATCGCGGTAACGGAGCATGAAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGAAGGAATGAGCGATTATCTGACGAAACCGAtt < 2:399868/99‑1 (MQ=255)
caacaaACGCCGGTTATCGCCGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTg > 2:1236456/1‑100 (MQ=255)
acaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaa > 2:1748011/1‑100 (MQ=255)
caaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGTCGAAACCGATTgaag > 1:1982343/1‑100 (MQ=255)
caaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaag < 2:149976/100‑1 (MQ=255)
aaCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaagaa < 2:134497/100‑1 (MQ=255)
aCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGaagaag < 1:279025/100‑1 (MQ=255)
tATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTg < 1:1748023/100‑1 (MQ=255)
aTCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGc < 1:1988875/100‑1 (MQ=255)
cgcgGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCAt < 2:1916607/100‑1 (MQ=255)
gcgGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATa < 1:1490481/100‑1 (MQ=255)
gcgGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATa < 2:1051609/100‑1 (MQ=255)
cgGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATaa < 1:1536206/100‑1 (MQ=255)
ggTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAAt < 2:112609/100‑1 (MQ=255)
gTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAAtt > 1:925566/1‑100 (MQ=255)
aaCGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttg < 2:772309/100‑1 (MQ=255)
aCGGCGCATGCAATGGCCGGGCAAAAAGATAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGTAACCGATTGAAGAAGAGCGATTGCATAATttgt < 2:1214103/100‑1 (MQ=255)
cGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgtt < 2:1543750/100‑1 (MQ=255)
cgcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgtt > 1:1626000/1‑100 (MQ=255)
cgcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgtt < 1:1013788/100‑1 (MQ=255)
gcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg < 1:216650/100‑1 (MQ=255)
gcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg < 1:1300318/100‑1 (MQ=255)
cATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgc > 2:588923/1‑100 (MQ=255)
gCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCt < 2:1390751/100‑1 (MQ=255)
gCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCt < 2:757857/100‑1 (MQ=255)
gCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGAGCt < 1:1236461/100‑1 (MQ=255)
tGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACaa < 2:70960/100‑1 (MQ=255)
tGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACaa > 2:141253/1‑100 (MQ=255)
tGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACa > 1:1805815/1‑99 (MQ=255)
gCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGc > 2:1867944/1‑100 (MQ=255)
ccGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGcc < 1:787563/100‑1 (MQ=255)
cGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCt < 1:1715676/100‑1 (MQ=255)
gCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGc < 1:20809/100‑1 (MQ=255)
aaGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCgg > 2:342861/1‑100 (MQ=255)
agagAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGt > 2:455372/1‑100 (MQ=255)
gaAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAtt < 2:876520/100‑1 (MQ=255)
gaAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAtt < 2:1982331/100‑1 (MQ=255)
aGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTc > 1:1990038/1‑100 (MQ=255)
ctgctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCct < 1:479672/100‑1 (MQ=255)
gctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctct > 1:388584/1‑100 (MQ=255)
ctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCtctc < 2:680973/100‑1 (MQ=255)
ggCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTcgcg > 1:1749759/1‑100 (MQ=255)
gcgcAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGcgt < 2:1780011/100‑1 (MQ=255)
gcAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGcgtcg > 2:1997544/1‑100 (MQ=255)
gcAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGcgtcg < 1:588923/100‑1 (MQ=255)
|
CTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCG > NC_000913/2917250‑2917439
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
