Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F201 I0 R1	306	176.9	8831342	93.4%	8248473	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,115,214	C→T	30.2%	K122K (AAG→AAA)	glpX ←	fructose 1,6‑bisphosphatase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,115,214	0	C	T	30.2%	238.0 / 109.1	158	K122K (AAG→AAA)	glpX	fructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand): ref base C (52/57); new base T (29/18); total (81/77)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

CTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATGCGC  >  NC_000913/4115116‑4115310

cTCAATGGTTTGCCGAGCGCCCCCGCTACATTGCGCATGTTATCCGCCAGCGTCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACCATCAGc                                                                                                  <  1:2898005/99‑1 (MQ=255)

cTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCt                                                                                                 <  1:3763558/100‑1 (MQ=255)

 tCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCtt                                                                                                >  1:1228472/1‑100 (MQ=255)

  cAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTtc                                                                                               >  2:3795722/1‑100 (MQ=255)

     cGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTcc                                                                                            <  1:1563028/100‑1 (MQ=255)

       gTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCat                                                                                          >  2:3231148/1‑100 (MQ=255)

       gTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCat                                                                                          >  2:2013009/1‑100 (MQ=255)

        tttGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCata                                                                                         <  2:2085815/100‑1 (MQ=255)

         ttGCCGCGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCatat                                                                                        <  1:3321962/100‑1 (MQ=255)

           gCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGACAGGTTCAGATCAATGGTGGCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATAc                                                                                      >  1:2403263/1‑100 (MQ=255)

                gcgcCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATc                                                                                 >  1:3895425/1‑100 (MQ=255)

                gcgcCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATc                                                                                 >  1:1962947/1‑100 (MQ=255)

                  gccgccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCg                                                                               >  2:1119393/1‑100 (MQ=255)

                   ccgccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTATCCATATACATCTCCg                                                                               >  1:180878/1‑99 (MQ=255)

                    cgccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGgc                                                                             <  2:2615237/100‑1 (MQ=255)

                      ccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGgcgc                                                                           >  1:679998/1‑100 (MQ=255)

                       cgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCa                                                                          >  2:3654886/1‑100 (MQ=255)

                          tACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTg                                                                       <  1:492092/100‑1 (MQ=255)

                           aCATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGa                                                                      >  2:1792474/1‑100 (MQ=255)

                           aCATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGa                                                                      <  2:890655/100‑1 (MQ=255)

                            cATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAg                                                                     <  1:517396/100‑1 (MQ=255)

                            cATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAg                                                                     <  2:3590116/100‑1 (MQ=255)

                             aTTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAgg                                                                    <  2:1962954/100‑1 (MQ=255)

                               tGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGacg                                                                    >  2:3931649/1‑96 (MQ=255)

                                 cgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAagc                                                                >  2:4008661/1‑100 (MQ=255)

                                  gcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAagca                                                               >  1:462811/1‑100 (MQ=255)

                                  gcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAagca                                                               <  2:1228476/100‑1 (MQ=255)

                                   cAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAagcag                                                              <  2:2650324/100‑1 (MQ=255)

                                       ttATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCtt                                                          <  2:253903/100‑1 (MQ=255)

                                       ttATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCtt                                                          >  2:1523869/1‑100 (MQ=255)

                                         aTCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGCAGCAGCCttt                                                         >  1:2522047/1‑99 (MQ=255)

                                          tCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTAt                                                       >  2:2555094/1‑100 (MQ=255)

                                           ccGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATc                                                      <  2:2298016/100‑1 (MQ=255)

                                           ccGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATc                                                      >  1:1770672/1‑100 (MQ=255)

                                            cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCg                                                     >  1:2252747/1‑100 (MQ=255)

                                            cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCg                                                     <  1:744937/100‑1 (MQ=255)

                                            cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCg                                                     >  1:809919/1‑100 (MQ=255)

                                            cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTAGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGAATTGAGGAAGCAGCCTTTATCg                                                     >  2:4182433/1‑100 (MQ=255)

                                             nccAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGc                                                    >  2:3556665/2‑100 (MQ=255)

                                             gCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAAACAGTTTCTCCATATACATATCCCTCGCCGTGAGTAAGCACGCTTTATCGc                                                    >  1:2421822/1‑100 (MQ=255)

                                              ccAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGcc                                                   <  2:4183452/100‑1 (MQ=255)

                                              ccAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGcc                                                   <  2:1015127/100‑1 (MQ=255)

                                               cAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCt                                                  <  2:4180804/100‑1 (MQ=255)

                                               cAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCt                                                  <  2:4011431/100‑1 (MQ=255)

                                                aGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTa                                                 <  1:2095019/100‑1 (MQ=255)

                                                 gCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTAc                                                >  1:1119301/1‑100 (MQ=255)

                                                   ggCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTg                                              >  1:1324992/1‑100 (MQ=255)

                                                   ggCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTg                                              <  1:2719092/100‑1 (MQ=255)

                                                   ggCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTg                                              <  2:3644756/100‑1 (MQ=255)

                                                    gCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGc                                             <  2:531624/100‑1 (MQ=255)

                                                    gCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGc                                             <  1:2559530/100‑1 (MQ=255)

                                                     cAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGcc                                            <  1:2390820/100‑1 (MQ=255)

                                                      aGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCa                                           <  1:4079847/100‑1 (MQ=255)

                                                      aGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCa                                           <  2:266549/100‑1 (MQ=255)

                                                       ggTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAg                                          <  1:930500/100‑1 (MQ=255)

                                                       ggTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAg                                          >  2:4330280/1‑100 (MQ=255)

                                                        gTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGc                                         >  2:1084736/1‑100 (MQ=255)

                                                         ttCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCa                                        >  1:792260/1‑100 (MQ=255)

                                                            aGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCg                                     >  2:2167162/1‑100 (MQ=255)

                                                            aGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCg                                     >  1:650233/1‑100 (MQ=255)

                                                            aGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCg                                     >  2:1388411/1‑100 (MQ=255)

                                                             gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCTCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                    >  2:4350877/1‑100 (MQ=255)

                                                             gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                    >  1:3286384/1‑100 (MQ=255)

                                                              aTCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGcc                                   <  2:3478835/100‑1 (MQ=255)

                                                               tCAATTGTGCCTTTGGCTCCCGGCGCGACAATGTGGTTCTCCATATACATATCAGGCGCATTGAGGTAGCAGCCTTTTTAGCCTACGGCGAGCACCg                                     <  1:1338060/97‑1 (MQ=255)

                                                                 aaTGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAgc                                >  2:1761211/1‑100 (MQ=255)

                                                                  gatctGCCTTTGGCTCCAGGCCAGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAgcg                               <  1:883900/96‑1 (MQ=255)

                                                                  aTGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAgcg                               >  2:2224545/1‑100 (MQ=255)

                                                                    ggTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGcgcg                             >  1:4369988/1‑100 (MQ=255)

                                                                      tGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGtt                           <  2:3879463/100‑1 (MQ=255)

                                                                       gCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGtt                           >  2:3340235/1‑99 (MQ=255)

                                                                       gCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTa                          <  1:1130791/100‑1 (MQ=255)

                                                                       cttccgatcTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTa                          <  1:3228417/92‑1 (MQ=255)

                                                                         cTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGc                        >  2:277562/1‑100 (MQ=255)

                                                                          tttGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGcc                       <  1:2792596/100‑1 (MQ=255)

                                                                             ggCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTgg                    >  1:3835018/1‑100 (MQ=255)

                                                                             ggCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTgg                    >  2:710345/1‑100 (MQ=255)

                                                                              gCTCCCGGCCTGACAATCACCTTCTCCATATACATATCCGGCGCATTAAGGAAGCAGCCTTTATCCTCTACTGGCACCACCGCCAGCTCGTTTGCCTg                     >  1:3676967/1‑98 (MQ=255)

                                                                              gCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                   <  2:832614/100‑1 (MQ=255)

                                                                              gCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                   <  1:3562662/100‑1 (MQ=255)

                                                                              gCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                   <  2:2999235/100‑1 (MQ=255)

                                                                               cTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGcc                  >  2:3686928/1‑100 (MQ=255)

                                                                               cTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGcc                  <  1:277561/100‑1 (MQ=255)

                                                                                tCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGccc                 >  2:3319318/1‑100 (MQ=255)

                                                                                 cccGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGcccc                 >  1:1227041/1‑99 (MQ=255)

                                                                                 cccGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa                >  2:1944206/1‑100 (MQ=255)

                                                                                 cccGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTaagatcggaag                >  2:3228435/1‑90 (MQ=255)

                                                                                 cccGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa                >  2:3167137/1‑100 (MQ=255)

                                                                                 cccGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa                >  1:484988/1‑100 (MQ=255)

                                                                                  ccGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCAt               <  2:2706630/100‑1 (MQ=255)

                                                                                    ggCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCg             <  2:4251734/100‑1 (MQ=255)

                                                                                    ggCCCGAAAATCAGCTTCTCCATATACGTATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCg             <  2:62608/100‑1 (MQ=255)

                                                                                       ccGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCg          >  1:2879274/1‑100 (MQ=255)

                                                                                       ccGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCg          >  1:2869308/1‑100 (MQ=255)

                                                                                         gACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTc        <  2:3810431/100‑1 (MQ=255)

                                                                                           cAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCAt      <  1:3167119/100‑1 (MQ=255)

                                                                                            aaTCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATg     <  2:2742485/100‑1 (MQ=255)

                                                                                             aTCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATgc    >  1:59675/1‑100 (MQ=255)

                                                                                              tCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATgcg   >  2:206054/1‑100 (MQ=255)

                                                                                               cAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCCGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATgcgc  >  1:2728664/1‑100 (MQ=255)

CTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATGCGC  >  NC_000913/4115116‑4115310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A