Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F258 I0 R1	432	103.0	5191697	92.7%	4812703	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,115,214	C→T	19.3%	K122K (AAG→AAA)	glpX ←	fructose 1,6‑bisphosphatase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,115,214	0	C	T	19.3%	280.9 / 52.4	129	K122K (AAG→AAA)	glpX	fructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand): ref base C (57/47); new base T (15/10); total (72/57)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 132 total aligned reads displayed.

GCTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATGCGCGTG  >  NC_000913/4115115‑4115313

gCTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGc                                                                                                     <  2:374411/100‑1 (MQ=255)

 cTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCt                                                                                                    >  2:176624/1‑100 (MQ=255)

 cTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCt                                                                                                    <  1:1348071/100‑1 (MQ=255)

   cAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTtc                                                                                                  >  1:855173/1‑100 (MQ=255)

   cAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTtc                                                                                                  <  2:1434487/100‑1 (MQ=255)

     aCGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTtctc                                                                                                <  2:450065/100‑1 (MQ=255)

      cGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGTTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTcc                                                                                               <  1:575607/100‑1 (MQ=255)

      cGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTcc                                                                                               <  2:2175396/100‑1 (MQ=255)

       ggTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCa                                                                                              <  2:20218/100‑1 (MQ=255)

           tGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCAtata                                                                                          >  2:832399/1‑100 (MQ=255)

           tGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCAtata                                                                                          <  1:336681/100‑1 (MQ=255)

            gCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATAc                                                                                         <  1:520307/100‑1 (MQ=255)

             cctaGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACa                                                                                        >  1:1774280/4‑100 (MQ=255)

             ccGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACa                                                                                        >  1:1350838/1‑100 (MQ=255)

              cGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACat                                                                                       <  1:958630/100‑1 (MQ=255)

              cGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACat                                                                                       >  2:1938400/1‑100 (MQ=255)

               gAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACata                                                                                      <  1:778718/100‑1 (MQ=255)

               gAGCGCCGCCGCTACATTGCGCAGGTTATCCACCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACata                                                                                      >  2:1750448/1‑100 (MQ=255)

                        cgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCa                                                                             >  2:85660/1‑100 (MQ=255)

                        cgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCa                                                                             <  2:868746/100‑1 (MQ=255)

                         gcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCAt                                                                            >  2:889465/1‑100 (MQ=255)

                           tACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTg                                                                          >  2:1178515/1‑100 (MQ=255)

                                 gcgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAag                                                                    >  2:575753/1‑100 (MQ=255)

                                 gcgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAag                                                                    >  2:1292594/1‑100 (MQ=255)

                                  cgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAagc                                                                   >  2:686483/1‑100 (MQ=255)

                                    cAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATAGCCGGCGCATTGAGGAagcag                                                                 <  2:2530502/100‑1 (MQ=255)

                                     aGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAagcagc                                                                <  2:295990/100‑1 (MQ=255)

                                      ggTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGcc                                                               <  2:1625402/100‑1 (MQ=255)

                                       gTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCt                                                              >  1:2383607/1‑100 (MQ=255)

                                       gTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCt                                                              >  2:784535/1‑100 (MQ=255)

                                        ttATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCtt                                                             >  1:1121315/1‑100 (MQ=255)

                                        ttATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCtt                                                             <  2:47803/100‑1 (MQ=255)

                                        ttATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCtt                                                             <  1:1961452/100‑1 (MQ=255)

                                          aTCCGCCAGCGGCACGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTa                                                           <  1:1764844/100‑1 (MQ=255)

                                           tCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTAt                                                          >  1:1178208/1‑100 (MQ=255)

                                           tCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTAt                                                          <  2:2483169/100‑1 (MQ=255)

                                            ccGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTAt                                                          >  1:395358/1‑99 (MQ=255)

                                            ccGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATc                                                         >  1:1068728/1‑100 (MQ=255)

                                             cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCTCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCg                                                        <  1:587876/100‑1 (MQ=255)

                                             cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCg                                                        >  2:718059/1‑100 (MQ=255)

                                             cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCg                                                        >  1:2421037/1‑100 (MQ=255)

                                              gCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGc                                                       >  2:2416735/1‑100 (MQ=255)

                                              gCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGc                                                       >  2:1038628/1‑100 (MQ=255)

                                               ccAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGcc                                                      <  2:399884/100‑1 (MQ=255)

                                                cAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCt                                                     <  1:1868510/100‑1 (MQ=255)

                                                cAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCt                                                     <  2:1423197/100‑1 (MQ=255)

                                                 aGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTa                                                    >  2:2513258/1‑100 (MQ=255)

                                                   cGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACt                                                  >  1:1482451/1‑100 (MQ=255)

                                                        ggTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAg                                             >  1:525180/1‑100 (MQ=255)

                                                        ggTTCAGAACAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGTCGCATTGAGGAAGCAGCCTTTTTCGCCTACTGCCAg                                             >  1:1542742/1‑100 (MQ=255)

                                                          ttCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCa                                           >  1:2481681/1‑100 (MQ=255)

                                                           tCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCAc                                          >  1:1867072/1‑100 (MQ=255)

                                                            cAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCAcc                                         >  1:1427563/1‑100 (MQ=255)

                                                             aGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCg                                        >  2:363864/1‑100 (MQ=255)

                                                              gATCAATGGTGCCTTTGGCTCCCGGCGCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                       >  2:1573800/1‑100 (MQ=255)

                                                              gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                       >  2:1001619/1‑100 (MQ=255)

                                                              gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                       >  1:1333202/1‑100 (MQ=255)

                                                              gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATGGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                       >  2:763821/1‑100 (MQ=255)

                                                                tCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCa                                     <  2:1271042/100‑1 (MQ=255)

                                                                 gatctgTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAg                                    <  1:2246077/95‑1 (MQ=255)

                                                                 cAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAg                                    >  2:1399340/1‑100 (MQ=255)

                                                                 cAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAg                                    <  2:1610392/100‑1 (MQ=255)

                                                                 cAATGGTGCCTTTGGCTCCCGGCCCGACAACCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCa                                     <  1:2057434/99‑1 (MQ=255)

                                                                     ggTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGcgcg                                <  2:1885520/100‑1 (MQ=255)

                                                                     ggTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGcgcg                                >  2:1300456/1‑100 (MQ=255)

                                                                      gTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATCCATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCACCACCGCCAGcgcg                                >  1:342636/1‑99 (MQ=255)

                                                                       tGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGtt                              >  2:2271457/1‑100 (MQ=255)

                                                                       tGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGtt                              <  1:1461132/100‑1 (MQ=255)

                                                                           tttGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAgag                          >  1:776716/1‑98 (MQ=255)

                                                                           tttGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGcc                          <  1:1850949/100‑1 (MQ=255)

                                                                           tttGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGcc                          <  1:176623/100‑1 (MQ=255)

                                                                            ttGGCTCCCGGCACGATAATCAGCTTCGCCATATACATAGTCGGCGCATTGAGGAAGCAGTTTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAg                            <  1:2327668/97‑1 (MQ=255)

                                                                              ggCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTgg                       >  1:673858/1‑100 (MQ=255)

                                                                              ggCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTgg                       <  2:1545766/100‑1 (MQ=255)

                                                                               gCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                      <  2:1005231/100‑1 (MQ=255)

                                                                               gCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                      >  2:755247/1‑100 (MQ=255)

                                                                                cTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCCGCACCGCCAGCGCGTTAGCCTGGc                      >  1:58724/1‑99 (MQ=255)

                                                                                cTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGcc                     >  2:838089/1‑100 (MQ=255)

                                                                                cTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGcc                     >  2:1956456/1‑100 (MQ=255)

                                                                                cTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGcc                     <  2:1565606/100‑1 (MQ=255)

                                                                                 tCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGATGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGcc                     <  1:1568778/99‑1 (MQ=255)

                                                                                  cccGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa                   <  1:1001616/100‑1 (MQ=255)

                                                                                  cccGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa                   >  2:112147/1‑100 (MQ=255)

                                                                                  cccGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa                   >  2:628557/1‑100 (MQ=255)

                                                                                  cccGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa                   >  2:1690566/1‑100 (MQ=255)

                                                                                    cGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATc                 >  2:2343715/1‑100 (MQ=255)

                                                                                    cGGCCCGACAATCAGCTTCCCCATATACATATCCGGCGCATTGAGGCAGCAGACTTTCTCGCCTACTGCCAGCACCGCCAGGGCTTTAGCCTGGCCCATc                 <  1:2342682/100‑1 (MQ=255)

                                                                                     ggCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCg                >  2:1302292/1‑100 (MQ=255)

                                                                                     ggCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCg                <  2:2245849/100‑1 (MQ=255)

                                                                                      gCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGc               <  2:2481700/100‑1 (MQ=255)

                                                                                        ccGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCg             >  2:1213470/1‑100 (MQ=255)

                                                                                         cGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGt            >  1:953087/1‑100 (MQ=255)

                                                                                           aCAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCa          <  1:2423161/100‑1 (MQ=255)

                                                                                            cAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCAt         <  1:889462/100‑1 (MQ=255)

                                                                                             aaTCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATg        <  2:1774289/100‑1 (MQ=255)

                                                                                              aTCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATgc       >  2:56155/1‑100 (MQ=255)

                                                                                              aTCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATgc       <  2:77455/100‑1 (MQ=255)

                                                                                                 aGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATGcgcg    >  2:595791/1‑100 (MQ=255)

                                                                                                  gCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGACTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGTCCATCGCCGTCATGcgcg    <  1:196476/99‑1 (MQ=255)

                                                                                                   cTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATGCGCGTg  <  1:146027/100‑1 (MQ=255)

GCTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATGCGCGTG  >  NC_000913/4115115‑4115313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A