Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F201 I1 R1 82 135.0 6815619 92.9% 6331710 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,115,214 C→T K122K (AAG→AAA)  glpX ← fructose 1,6‑bisphosphatase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,115,2140CT98.3% 402.4 / ‑2.6 117K122K (AAG→AAA) glpXfructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (59/56);  total (59/58)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 117 total aligned reads displayed.

CAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATGCG  >  NC_000913/4115118‑4115309
                                                                                                |                                                                                               
cAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTtc                                                                                              <  1:146542/100‑1 (MQ=255)
cAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTtc                                                                                              >  1:1126221/1‑100 (MQ=255)
cAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTtc                                                                                              <  2:1385862/100‑1 (MQ=255)
cAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTtc                                                                                              >  2:335657/1‑100 (MQ=255)
cAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTtc                                                                                              >  2:2479807/1‑100 (MQ=255)
 aaCGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTtct                                                                                             >  1:3232697/1‑100 (MQ=255)
  aCGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTtctc                                                                                            >  2:1785336/1‑100 (MQ=255)
  aCGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTtctc                                                                                            <  1:3395159/100‑1 (MQ=255)
   cGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCCGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTcc                                                                                           <  1:431359/100‑1 (MQ=255)
   cGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTcc                                                                                           <  2:1508824/100‑1 (MQ=255)
   cGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTcc                                                                                           <  1:436912/100‑1 (MQ=255)
   cGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTcc                                                                                           <  1:3325028/100‑1 (MQ=255)
       ttGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCatat                                                                                       <  1:109561/100‑1 (MQ=255)
        tGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCAtata                                                                                      <  2:636695/100‑1 (MQ=255)
        tGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCAtata                                                                                      <  2:1323121/100‑1 (MQ=255)
        tGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCAtata                                                                                      >  1:636695/1‑100 (MQ=255)
        tGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCAtata                                                                                      <  2:89465/100‑1 (MQ=255)
        tGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCAtata                                                                                      <  1:1199939/100‑1 (MQ=255)
          ccGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACa                                                                                    >  1:1081860/1‑100 (MQ=255)
          ccGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACa                                                                                    <  1:1270174/100‑1 (MQ=255)
          ccGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACa                                                                                    <  1:570196/100‑1 (MQ=255)
           cGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACat                                                                                   >  2:13024/1‑100 (MQ=255)
            gAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACata                                                                                  <  1:3326970/100‑1 (MQ=255)
               cgccgccgcTACATTGCGCAGGTTATCCGCCAGCGTCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTTTCCATATACatat                                                                                 <  1:2313071/98‑1 (MQ=255)
                  cgccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGgc                                                                            >  1:2049634/1‑100 (MQ=255)
                   gccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGgcg                                                                           <  1:399238/100‑1 (MQ=255)
                   gccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGgcg                                                                           <  1:308173/100‑1 (MQ=255)
                      gcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCATATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCAGGCGCAt                                                                        >  2:1835050/1‑100 (MQ=255)
                      gcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATGAATGGTGCCTTTGGCTCCCGGCCCGACACTCAGTTTCTCCATATACATATCCGGCGCAt                                                                        >  2:1900817/1‑100 (MQ=255)
                      gcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCAt                                                                        >  1:2799996/1‑100 (MQ=255)
                        tACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTg                                                                      <  2:1081859/100‑1 (MQ=255)
                          cATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAg                                                                    <  2:3232684/100‑1 (MQ=255)
                          cATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAg                                                                    <  1:2703086/100‑1 (MQ=255)
                           aTTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAgg                                                                   <  2:365178/100‑1 (MQ=255)
                              gcgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAag                                                                >  2:1647251/1‑100 (MQ=255)
                              gcgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAag                                                                >  2:2973303/1‑100 (MQ=255)
                              gcgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAag                                                                <  1:1941459/100‑1 (MQ=255)
                               cgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAagc                                                               >  2:1327708/1‑100 (MQ=255)
                               cgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAagc                                                               >  2:3090863/1‑100 (MQ=255)
                               cgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAagc                                                               >  2:2450786/1‑100 (MQ=255)
                                 tccgatctCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAagcag                                                             <  2:3359262/93‑1 (MQ=255)
                                 cAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAagcag                                                             >  2:1573186/1‑100 (MQ=255)
                                 cAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAagcag                                                             >  2:3142704/1‑100 (MQ=255)
                                 cAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAagcag                                                             >  2:3362914/1‑100 (MQ=255)
                                     ttATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCtt                                                         >  2:2182405/1‑100 (MQ=255)
                                     ttATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCtt                                                         >  1:3011519/1‑100 (MQ=255)
                                       aTCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTa                                                       <  1:1647253/100‑1 (MQ=255)
                                         ccGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAagcagagatcgga                                                     >  1:3359275/1‑92 (MQ=255)
                                         ccGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATc                                                     <  2:611034/100‑1 (MQ=255)
                                          cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCg                                                    >  2:3385541/1‑100 (MQ=255)
                                           gCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATcag                                                   >  1:611035/1‑98 (MQ=255)
                                            ccAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGcc                                                  <  1:1785340/100‑1 (MQ=255)
                                            ccAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGcc                                                  <  2:1581264/100‑1 (MQ=255)
                                             cAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCt                                                 <  1:2103453/100‑1 (MQ=255)
                                             cAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCt                                                 <  2:433081/100‑1 (MQ=255)
                                              aGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTa                                                >  1:422710/1‑100 (MQ=255)
                                               gCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTAc                                               <  1:801994/100‑1 (MQ=255)
                                                     ggTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAg                                         <  2:3296647/100‑1 (MQ=255)
                                                     ggTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAg                                         <  1:937316/100‑1 (MQ=255)
                                                      gTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGc                                        <  1:2479815/100‑1 (MQ=255)
                                                       ttCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCa                                       >  2:1346789/1‑100 (MQ=255)
                                                         cAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCAcc                                     <  1:1900821/100‑1 (MQ=255)
                                                         cAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCAcc                                     <  2:657008/100‑1 (MQ=255)
                                                           gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                   >  1:2452149/1‑100 (MQ=255)
                                                           gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                   >  1:3137777/1‑100 (MQ=255)
                                                           gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                   >  2:1408982/1‑100 (MQ=255)
                                                           gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                   >  2:892087/1‑100 (MQ=255)
                                                            aTCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGcc                                  <  1:289866/100‑1 (MQ=255)
                                                            aTCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGcc                                  <  1:341674/100‑1 (MQ=255)
                                                              cAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAg                                <  1:3142716/100‑1 (MQ=255)
                                                              cAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAg                                <  1:3362927/100‑1 (MQ=255)
                                                               aaTGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAgc                               >  1:1865080/1‑100 (MQ=255)
                                                                aTGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAgcg                              <  1:1436373/100‑1 (MQ=255)
                                                                aTGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAgcg                              <  1:493722/100‑1 (MQ=255)
                                                                  ggTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCaagatc                            >  2:1748909/1‑95 (MQ=255)
                                                                  ggTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGcgcg                            >  1:2966495/1‑100 (MQ=255)
                                                                    tGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGtt                          <  2:108829/100‑1 (MQ=255)
                                                                      ccTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAg                        >  1:1583865/1‑100 (MQ=255)
                                                                       cTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGc                       >  1:668549/1‑100 (MQ=255)
                                                                        tttGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGcc                      >  2:1142745/1‑100 (MQ=255)
                                                                        tttGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGcc                      >  2:14896/1‑100 (MQ=255)
                                                                         ttGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCt                     <  1:1327711/100‑1 (MQ=255)
                                                                          tGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTg                    <  2:1749612/100‑1 (MQ=255)
                                                                            gCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                  <  2:2925392/100‑1 (MQ=255)
                                                                            gCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                  >  2:1718622/1‑100 (MQ=255)
                                                                            gCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                  >  2:1628432/1‑100 (MQ=255)
                                                                            gCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                  >  2:2472128/1‑100 (MQ=255)
                                                                            gCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                  >  1:2925403/1‑100 (MQ=255)
                                                                             cTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGcc                 >  1:725158/1‑100 (MQ=255)
                                                                             cTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGcc                 >  1:677050/1‑100 (MQ=255)
                                                                               cccGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa               >  1:223708/1‑100 (MQ=255)
                                                                                  ggCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCg            >  1:1155430/1‑100 (MQ=255)
                                                                                  ggCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCg            >  1:3013240/1‑100 (MQ=255)
                                                                                     ccGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCg         >  1:227864/1‑100 (MQ=255)
                                                                                      cGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGt        <  1:1464914/100‑1 (MQ=255)
                                                                                         ctaTCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCAt     <  1:2681539/98‑1 (MQ=255)
                                                                                         cAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCAt     >  2:2260205/1‑100 (MQ=255)
                                                                                           aTCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCAtag   >  2:2681530/1‑98 (MQ=255)
                                                                                           aTCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATgc   >  1:973676/1‑100 (MQ=255)
                                                                                            tCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATgcg  <  1:2214078/100‑1 (MQ=255)
                                                                                                |                                                                                               
CAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATGCG  >  NC_000913/4115118‑4115309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A