mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 107,553 | (T)9→8 | intergenic (+79/‑152) | lpxC → / → secM | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation |
| RA | 236,569 | C→T | A168V (GCC→GTC) | dnaQ → | DNA polymerase III epsilon subunit |
| RA | 238,990 | C→T | pseudogene (113/357 nt) | yafU ← | pseudogene |
| MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
| RA | 284,372 | Δ1 bp | coding (1172/1968 nt) | yagF → | CP4‑6 prophage; dehydratase family protein |
| RA | 380,021 | (G)10→8 | intergenic (‑140/+47) | frmR ← / ← yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| RA | 410,479 | A→G | S112S (TCA→TCG) | mak → | manno(fructo)kinase |
| RA | 420,470 | A→G | T294A (ACG→GCG) | brnQ → | branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val |
| RA | 426,471 | G→A | R112H (CGT→CAT) | tgt → | tRNA‑guanine transglycosylase |
| RA | 432,193 | +G | intergenic (‑180/+119) | tsx ← / ← yajI | nucleoside channel, receptor of phage T6 and colicin K/putative lipoprotein |
| RA | 507,147 | (A)8→9 | intergenic (‑65/+139) | ybaK ← / ← ybaP | Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase/TraB family protein |
| RA | 561,696 | (G)6→7 | coding (2000/2604 nt) | sfmD → | putative outer membrane export usher protein |
| RA | 669,244 | T→C | intergenic (‑208/+52) | rsfS ← / ← cobC | ribosomal silencing factor/putative alpha‑ribazole‑5'‑P phosphatase |
| RA | 715,849 | A→G | L146L (TTA→TTG) | ybfP → | lipoprotein |
| RA | 741,817 | C→T | G247D (GGC→GAC) | dtpD ← | dipeptide and tripeptide permease D |
| RA | 953,802 | A→G | V222A (GTA→GCA) | focA ← | formate channel |
| RA | 954,813 | (A)7→6 | intergenic (‑347/+59) | focA ← / ← ycaO | formate channel/ribosomal protein S12 methylthiotransferase accessory factor |
| RA | 1,053,516 | (G)6→7 | coding (2663/2745 nt) | torS ← | hybrid sensory histidine kinase in two‑component regulatory system with TorR |
| RA | 1,143,641 | G→A | H243Y (CAT→TAT) | rne ← | endoribonuclease; RNA‑binding protein;RNA degradosome binding protein |
| RA | 1,197,853 | T→C | N129D (AAT→GAT) | ymfE ← | e14 prophage; putative inner membrane protein |
| RA | 1,212,535 | A→G | intergenic (‑532/+168) | iraM ← / ← ycgX | RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein |
| RA | 1,246,073 | C→T | G435D (GGC→GAC) | treA ← | periplasmic trehalase |
| RA | 1,489,197 | C→T | D322D (GAC→GAT) | aldA → | aldehyde dehydrogenase A, NAD‑linked |
| RA | 1,594,256 | (C)8→7 | pseudogene (3732/3861 nt) | yneO ← | pseudogene, AidA homolog |
| RA | 1,602,117 | A→G | I210V (ATT→GTT) | lsrA → | autoinducer 2 import ATP‑binding protein |
| RA | 1,615,188 | A→G | T129A (ACT→GCT) | yneJ → | putative DNA‑binding transcriptional regulator |
| RA | 1,698,281 | A→G | V300V (GTT→GTC) | malI ← | transcriptional repressor of Mal regulon |
| RA | 1,730,999 | T→C | F638S (TTT→TCT) | lhr → | putative ATP‑dependent helicase |
| RA | 1,755,639 | G→A | intergenic (‑498/‑59) | ydhZ ← / → pykF | uncharacterized protein/pyruvate kinase I |
| RA | 1,755,705 | (A)5→4 | coding (8/1413 nt) | pykF → | pyruvate kinase I |
| RA | 1,868,250 | A→G | Y448C (TAC→TGC) | yeaG → | protein kinase, endogenous substrate unidentified; autokinase |
| RA | 1,903,571 | A→G | T164A (ACC→GCC) | manY → | mannose‑specific enzyme IIC component of PTS |
| MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
| JC JC | 1,979,486 | IS5 (+) +4 bp | intergenic (‑271/‑264) | insA ← / → uspC | IS1 repressor TnpA/universal stress protein |
| RA | 2,018,260 | (A)8→7 | coding (315/444 nt) | fliJ → | flagellar protein |
| RA | 2,127,857 | G→A | P153L (CCG→CTG) | gmd ← | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| RA | 2,333,850 | (T)6→7 | pseudogene (553/4605 nt) | yfaS ← | pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein |
| RA | 2,469,733 | (T)8→7 | coding (603/1332 nt) | gtrS → | serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage |
| RA | 2,470,410 | (T)7→6 | coding (1280/1332 nt) | gtrS → | serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage |
| RA | 2,494,930 | (C)5→6 | coding (233/276 nt) | ypdI → | putative lipoprotein involved in colanic acid biosynthesis |
| RA | 2,516,689 | T→C | T382A (ACG→GCG) | yfeA ← | putative diguanylate cyclase |
| RA | 2,553,210 | A→G | K287E (AAA→GAA) | amiA → | N‑acetylmuramoyl‑l‑alanine amidase I |
| RA | 2,651,755 | G→A | P178L (CCT→CTT) | yfhM ← | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| RA | 2,652,855 | (G)6→7 | coding (362/846 nt) | sseA → | 3‑mercaptopyruvate sulfurtransferase |
| RA | 2,654,545 | A→G | L130P (CTG→CCG) | sseB ← | rhodanase‑like enzyme, sulfur transfer from thiosulfate |
| RA | 2,731,312 | G→A | intergenic (‑155/+288) | rrsG ← / ← clpB | 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone |
| RA | 2,737,721 | (T)6→7 | intergenic (+75/‑24) | pheL → / → pheA | pheA gene leader peptide/chorismate mutase and prephenate dehydratase, P‑protein |
| RA | 2,776,109 | (G)5→4 | coding (191/459 nt) | yfjX → | CP4‑57 prophage; putative antirestriction protein |
| RA | 2,809,774 | (T)7→8 | coding (158/738 nt) | ygaZ → | putative L‑valine exporter, norvaline resistance protein |
| RA | 2,866,592 | +C | coding (960/993 nt) | rpoS ← | RNA polymerase, sigma S (sigma 38) factor |
| RA | 2,947,713 | (T)7→8 | intergenic (+30/+44) | metV → / ← amiC | tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase |
| RA | 3,008,075 | A→G | D189G (GAC→GGC) | ygeX → | 2,3‑diaminopropionate ammonia lyase, PLP‑dependent |
| RA | 3,182,712 | G→A | A55T (GCC→ACC) | zupT → | zinc transporter |
| RA | 3,194,516 | A→G | K551K (AAA→AAG) | yqiK → | PHB family membrane protein, function unknown |
| RA | 3,322,662 | (A)7→6 | intergenic (‑157/+71) | secG ← / ← glmM | preprotein translocase membrane subunit/phosphoglucosamine mutase |
| RA | 3,352,673 | T→C | E118G (GAA→GGA) | arcB ← | aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB |
| RA | 3,361,104 | T→C | intergenic (+488/‑72) | gltD → / → gltF | glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein |
| RA | 3,379,266 | (C)7→8 | coding (732/1128 nt) | zapE ← | divisome ATPase |
| RA | 3,411,299 | T→C | V10A (GTA→GCA) | fis → | global DNA‑binding transcriptional dual regulator |
| RA | 3,512,561 | Δ1 bp | intergenic (‑217/+73) | yhfZ ← / ← trpS | putative DNA‑binding transcriptional regulator/tryptophanyl‑tRNA synthetase |
| RA | 3,542,093 | G→A | V563M (GTG→ATG) | feoB → | ferrous iron transporter protein B and GTP‑binding protein; membrane protein |
| RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| MC JC | 3,582,206 | Δ1,222 bp | IS1‑mediated | [yhhZ]–yrhA | [yhhZ], yrhA |
| RA | 3,595,315 | (A)5→4 | coding (166/1278 nt) | livM ← | branched‑chain amino acid ABC transporter permease |
| RA | 3,648,144 | (T)7→6 | intergenic (‑356/‑384) | dinQ ← / → arsR | UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor |
| MC JC | 3,815,859 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
| RA | 3,952,380 | G→A | L60L (CTG→CTA) | ilvM → | acetolactate synthase 2 small subunit |
| RA | 3,952,569 | C→T | A29V (GCG→GTG) | ilvE → | branched‑chain amino acid aminotransferase |
| RA | 4,103,769 | A→G | F403L (TTC→CTC) | cpxA ← | sensory histidine kinase in two‑component regulatory system with CpxR |
| RA | 4,115,214 | C→T | K122K (AAG→AAA) | glpX ← | fructose 1,6‑bisphosphatase II |
| RA | 4,122,367 | (G)7→8 | intergenic (‑80/+13) | hslV ← / ← ftsN | peptidase component of the HslUV protease/essential cell division protein |
| RA | 4,214,964 | A→G | I229V (ATT→GTT) | metA → | homoserine O‑transsuccinylase |
| RA | 4,296,060 | C→T | intergenic (+266/+376) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| RA | 4,337,024 | G→A | S3L (TCG→TTG) | adiC ← | arginine:agmatine antiporter |
| RA | 4,397,557 | G→T | G49V (GGT→GTT) | mutL → | methyl‑directed mismatch repair protein |
| RA | 4,410,053 | (T)9→8 | intergenic (+47/‑80) | rlmB → / → yjfI | 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein |
| RA | 4,494,883 | (A)7→8 | coding (261/564 nt) | idnK → | D‑gluconate kinase, thermosensitive |
| RA | 4,573,528 | A→G | pseudogene (1115/1503 nt) | yjiT → | pseudogene |
| RA | 4,606,215 | (G)8→9 | noncoding (72/87 nt) | leuP ← | tRNA‑Leu |
| RA | 4,638,193 | (C)6→7 | coding (16/1353 nt) | creD → | inner membrane protein |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 3423705–3424234 | 3424589–3424238 | 5–885 | 73 [69] | [72] 73 | [rrfD]–[rrlD] | [rrfD], [rrlD] |
| * | * | ÷ | NC_000913 | 3621158–3622298 | 3622668–3622421 | 124–1511 | 74 [67] | [72] 75 | rhsB | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 1299498 | 0 (0.000) | 144 (1.120) | 91/190 | 0.1 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
| ? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||