Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,240,754	(C)_5→6	coding (915/1041 nt)	galS ←	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,240,749	1	.	C	96.7%	208.2 / ‑0.6	61	coding (920/1041 nt)	galS	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (27/31); total (30/31)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑CCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA  >  NC_000913/2240614‑2240880

caGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑ccc                                                                                                                                    <  1:1276237/139‑1 (MQ=255)

     tGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctg                                                                                                                               <  2:955120/139‑4 (MQ=255)

      gAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc                                                                                                                              <  2:700588/139‑5 (MQ=255)

             gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCa                                                                                                                        >  2:1323892/1‑139 (MQ=255)

             gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCa                                                                                                                        >  2:800759/1‑139 (MQ=255)

             gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCa                                                                                                                        >  1:290032/1‑139 (MQ=255)

             gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc                                                                                                                              >  2:529750/1‑128 (MQ=255)

             gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc                                                                                                                              >  2:546941/1‑128 (MQ=255)

             gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc                                                                                                                              <  1:546941/132‑5 (MQ=255)

             gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc                                                                                                                              <  1:529750/132‑5 (MQ=255)

                 cATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTc                                                                                                                   <  1:1323892/139‑1 (MQ=255)

                     gCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc                                                                                                                              >  2:1340823/1‑120 (MQ=255)

                     gCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc                                                                                                                              <  1:1340823/124‑5 (MQ=255)

                      ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTgg                                                                                                              >  2:1012796/1‑139 (MQ=255)

                              gttgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc                                                                                                                              >  2:182695/1‑111 (MQ=255)

                              gttgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc                                                                                                                              <  1:182695/115‑5 (MQ=255)

                               ttgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATCCTG‑CTGC‑‑‑‑CCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑At                                                                                                >  1:1452587/1‑139 (MQ=255)

                                      aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑a                                                                                                 >  2:347233/1‑135 (MQ=255)

                                      aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑a                                                                                                 <  1:347233/135‑1 (MQ=255)

                                      aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCCAATTTCGCC‑ATTGa                                                                                             <  2:290032/139‑1 (MQ=255)

                                         gATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGc                                                                                          >  1:141412/1‑139 (MQ=255)

                                           tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCaa                                                                                        <  1:800759/139‑1 (MQ=255)

                                            cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAg                                                                                           >  2:1109095/1‑135 (MQ=255)

                                            cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAg                                                                                           <  1:1109095/135‑1 (MQ=255)

                                             gccgctgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGTCAG‑TTCGGTGGCTAATTTCGCC‑ATTGa                                                                                             <  2:916135/132‑1 (MQ=255)

                                             gccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGG‑CCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGa                                                                                             >  1:916135/1‑132 (MQ=255)

                                                     aTTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACg                                                                              <  2:141412/139‑1 (MQ=255)

                                                       tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCATGGCCAG‑TTCGTTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCa                                                                            <  1:976648/139‑1 (MQ=255)

                                                       tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCa                                                                            <  2:1131943/139‑1 (MQ=255)

                                                         tGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACg                                                                          <  1:142525/139‑1 (MQ=255)

                                                                ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTa                                                                   >  2:1225472/1‑139 (MQ=255)

                                                                        gAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCggg                                                           <  2:989425/139‑1 (MQ=255)

                                                                         agtGGCGACGCACTAACGTCGGCATAAAACAGTGGCAGGCACGAGGATTAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTACGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt                                                          <  1:890499/137‑1 (MQ=255)

                                                                          aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc                                                         <  1:1388253/139‑1 (MQ=255)

                                                                                 cGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAg                                                                                           <  2:1365943/98‑1 (MQ=255)

                                                                                 cGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAg                                                                                           >  1:1365943/1‑98 (MQ=255)

                                                                                 cGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTaa                                                  >  1:1264937/1‑139 (MQ=255)

                                                                                   cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCg                                                                                                                  >  2:603156/1‑74 (MQ=255)

                                                                                   cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCg                                                                                                                  <  1:603156/74‑1 (MQ=255)

                                                                                   cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACg                                                >  1:282651/1‑139 (MQ=255)

                                                                                   cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACg                                                >  2:849494/1‑139 (MQ=255)

                                                                                   cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGACAACGCACGGTCGTTAATTGCGGGTCGGTGTAACg                                                >  1:740252/1‑139 (MQ=255)

                                                                                     cTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACggg                                              <  2:1478075/139‑1 (MQ=255)

                                                                                      tAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc                                             <  1:1225472/139‑1 (MQ=255)

                                                                                      tAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GACCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCCATGGGATAACGCACGGTCGTTAATTGCGGGGCGGTGTAACggct                                             >  2:890499/1‑137 (MQ=255)

                                                                                       aaCGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCg                                            <  1:1012796/139‑1 (MQ=255)

                                                                                         cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                          <  1:299098/139‑1 (MQ=255)

                                                                                         cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                          <  1:127898/139‑1 (MQ=255)

                                                                                             ggCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGa                                      <  2:1264937/139‑1 (MQ=255)

                                                                                              gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTaa                                                  >  1:1209204/1‑126 (MQ=255)

                                                                                              gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTaa                                                  <  2:1209204/126‑1 (MQ=255)

                                                                                                aTAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAata                                   <  1:1068133/139‑1 (MQ=255)

                                                                                                         tggctggcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACg                                                                          >  1:659988/1‑91 (MQ=255)

                                                                                                         tggctggcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACg                                                                          <  2:659988/91‑1 (MQ=255)

                                                                                                          ggctggcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCa                                                                            <  2:790985/88‑1 (MQ=255)

                                                                                                          ggctggcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCa                                                                            >  1:790985/1‑88 (MQ=255)

                                                                                                             tggcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                      <  1:849494/139‑1 (MQ=255)

                                                                                                              ggcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCa                                                                                                                        <  1:447924/42‑1 (MQ=255)

                                                                                                              ggcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCa                                                                                                                        >  2:447924/1‑42 (MQ=255)

                                                                                                              ggcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgat                     >  1:1334800/1‑139 (MQ=255)

                                                                                                                cACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCa                                                                            <  2:983514/82‑1 (MQ=255)

                                                                                                                cACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCa                                                                            >  1:983514/1‑82 (MQ=255)

                                                                                                                 aCGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgatgat                  >  2:1056898/1‑139 (MQ=255)

                                                                                                                          aaatttccttgct‑g‑cCCCCTGCAAAGCCCGTTTCGTTGGC‑AATTTCGCCAATTGAAGCAATGGG‑TAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGt        <  1:88590/130‑1 (MQ=255)

                                                                                                                              ttGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAAc     >  1:1387242/1‑139 (MQ=255)

                                                                                                                               tGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGc                                                                                                    <  2:301964/44‑1 (MQ=255)

                                                                                                                               tGCCTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGc                                                                                                    >  1:301964/1‑44 (MQ=255)

                                                                                                                                 ccTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                          <  2:1116765/99‑1 (MQ=255)

                                                                                                                                 ccTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                          >  1:1116765/1‑99 (MQ=255)

                                                                                                                                 ccTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg                       <  1:962338/118‑1 (MQ=255)

                                                                                                                                 ccTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg                       >  2:962338/1‑118 (MQ=255)

                                                                                                                                 ccTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa  >  2:1118867/1‑139 (MQ=255)

                                                                                                                                 ccTGCT‑GCCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa  >  2:128832/1‑139 (MQ=255)

                                                                                                                                   tgct‑gcCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCaa                                                                                        <  1:652422/51‑1 (MQ=255)

                                                                                                                                   tgct‑gcCCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCaa                                                                                        >  2:652422/1‑51 (MQ=255)

CAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑CCCCCTGCAAGGCCAG‑TTCGGTGGCTAATTTCGCC‑ATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA  >  NC_000913/2240614‑2240880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A