Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A9 F1 I2 R1 | 760 | 52.7 | 2744488 | 90.2% | 2475528 | 105.0 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,240,754 | (C)5→6 | coding (915/1041 nt) | galS ← | galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,240,749 | 1 | . | C | 98.1% | 180.7 / ‑3.5 | 52 | coding (920/1041 nt) | galS | galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (23/28); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
CACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑C‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA > NC_000913/2240612‑2240880 | cacaGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCtgctg‑c > 2:1125548/1‑139 (MQ=255) tGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccccctg < 2:117974/139‑4 (MQ=255) cGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGc < 2:436756/139‑1 (MQ=255) ggTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGcc > 2:1347665/1‑139 (MQ=255) aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa > 2:460226/1‑139 (MQ=255) tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa < 2:619759/139‑1 (MQ=255) gccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGTAGCAATGGGa < 1:810214/139‑1 (MQ=255) ccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGAt > 1:56275/1‑139 (MQ=255) cgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATa > 1:23228/1‑139 (MQ=255) tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:476268/139‑1 (MQ=255) tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 2:1151628/139‑1 (MQ=255) tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 2:23228/139‑1 (MQ=255) tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 2:56275/139‑1 (MQ=255) tGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg < 1:182651/139‑1 (MQ=255) tGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg > 1:684404/1‑139 (MQ=255) gtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGtt < 1:1347665/139‑1 (MQ=255) ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTa > 1:249434/1‑139 (MQ=255) tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTaa > 2:463467/1‑139 (MQ=255) cGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTCCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAAtt > 1:982871/1‑139 (MQ=255) aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa > 2:1015294/1‑104 (MQ=255) aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa < 1:1015294/104‑1 (MQ=255) aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc < 2:1121094/139‑1 (MQ=255) tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCg < 2:27691/82‑1 (MQ=255) tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCg > 1:27691/1‑82 (MQ=255) cGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccccctgc > 1:134058/1‑60 (MQ=255) cGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccccctgc < 2:134058/64‑5 (MQ=255) cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAAttt > 2:688595/1‑85 (MQ=255) cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAAttt < 1:688595/85‑1 (MQ=255) cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa > 2:284727/1‑111 (MQ=255) cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:284727/111‑1 (MQ=255) aCTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACgg < 1:338784/139‑1 (MQ=255) cTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACggg < 1:463467/139‑1 (MQ=255) cGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccc > 1:1153214/1‑48 (MQ=255) cGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccc < 2:1153214/48‑1 (MQ=255) ggCATAAAACAGTGGCTGGGACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc < 1:1012690/132‑1 (MQ=255) ggCATAAAACAGTGGCTGGGACGAGGATCAATATTGCCTGCTG‑CACCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc > 2:1012690/1‑132 (MQ=255) ggCATAAAACAGTGGCTGGCACGAGGATCAATATTGCctgctg > 2:804722/1‑43 (MQ=255) ggCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTc < 1:477243/76‑1 (MQ=255) ggCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTc > 2:477243/1‑76 (MQ=255) ggCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc < 2:1287817/120‑1 (MQ=255) ggCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc > 1:1287817/1‑120 (MQ=255) ggCATAAAACAGTGGCTGGCACAAGGATCAATATTGCctgctg < 1:804722/43‑1 (MQ=255) gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCg > 1:300540/1‑63 (MQ=255) gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCg < 2:300540/63‑1 (MQ=255) cATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAat < 1:379821/139‑1 (MQ=255) tggctggcACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGaaa > 2:451845/1‑139 (MQ=255) ctggcACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg > 2:587862/1‑139 (MQ=255) tggcACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga < 2:249434/139‑1 (MQ=255) cACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgatga > 2:43036/1‑139 (MQ=255) ggATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag < 2:484810/139‑1 (MQ=255) tCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgagaga < 1:460226/139‑1 (MQ=255) atatTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt > 1:168523/1‑105 (MQ=255) atatTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt < 2:168523/105‑1 (MQ=255) tGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa > 1:1158015/1‑137 (MQ=255) tGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa < 2:1158015/137‑1 (MQ=255) gCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg > 1:1221737/1‑51 (MQ=255) gCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg < 2:1221737/51‑1 (MQ=255) gCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 2:138537/66‑1 (MQ=255) gCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa > 1:138537/1‑66 (MQ=255) ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa > 1:874019/1‑139 (MQ=255) ctgctgcC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa > 2:635084/1‑64 (MQ=255) ctgctgcC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:635084/64‑1 (MQ=255) | CACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑C‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA > NC_000913/2240612‑2240880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |