Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,240,754	(C)_5→6	coding (915/1041 nt)	galS ←	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,240,749	1	.	C	100.0%	155.0 / NA	43	A307G (GCA→GGA)	galS	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (15/28); total (15/28)

TCACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑C‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAT  >  NC_000913/2240611‑2240882

tcacAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCctgctg                                                                                                                                         <  1:1053072/139‑1 (MQ=255)

tcacAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCctgctg                                                                                                                                         <  2:700616/139‑1 (MQ=255)

      aTTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccccc                                                                                                                                 >  1:881597/1‑138 (MQ=255)

            aGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAg                                                                                                                            <  1:787974/139‑1 (MQ=255)

                gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCa                                                                                                                        >  1:840650/1‑139 (MQ=255)

                gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCa                                                                                                                        >  2:247715/1‑139 (MQ=255)

                gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCa                                                                                                                        >  2:952929/1‑139 (MQ=255)

                gTTACATCGCCAGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGCCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CTCCCCTGCAAGGCCa                                                                                                                        >  2:716602/1‑139 (MQ=255)

                   aCATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGtt                                                                                                                     <  1:952929/139‑1 (MQ=255)

                                         aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                               <  1:206081/139‑1 (MQ=255)

                                         aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                               <  2:140102/139‑1 (MQ=255)

                                           tGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg                                                                                             <  2:282234/139‑1 (MQ=255)

                                             aTCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCa                                                                                           <  2:840650/139‑1 (MQ=255)

                                              tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                          <  1:123609/139‑1 (MQ=255)

                                                   gccgtATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAACACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                     <  1:696986/139‑1 (MQ=255)

                                                   gccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                     <  1:239997/139‑1 (MQ=255)

                                                   gccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                     <  1:308763/139‑1 (MQ=255)

                                                          tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              <  2:880689/139‑1 (MQ=255)

                                                          tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              <  2:204007/139‑1 (MQ=255)

                                                                   ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCg                                                                                                      >  2:979805/1‑106 (MQ=255)

                                                                   ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCg                                                                                                      <  1:979805/106‑1 (MQ=255)

                                                                           gAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCggg                                                             <  1:1031257/139‑1 (MQ=255)

                                                                                   aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTa                                                     >  2:529603/1‑139 (MQ=255)

                                                                                    cGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTaa                                                    <  1:965584/139‑1 (MQ=255)

                                                                                    cGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTaa                                                    <  1:445280/139‑1 (MQ=255)

                                                                                      cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACg                                                  <  1:424537/139‑1 (MQ=255)

                                                                                            cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                            <  2:881597/139‑1 (MQ=255)

                                                                                            cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                            <  1:434617/139‑1 (MQ=255)

                                                                                                ggCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGa                                        >  1:860566/1‑139 (MQ=255)

                                                                                                 gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                     >  2:735806/1‑93 (MQ=255)

                                                                                                 gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                     <  1:735806/93‑1 (MQ=255)

                                                                                                 gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccc                                                                                                                                   <  2:46059/46‑1 (MQ=255)

                                                                                                 gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccc                                                                                                                                   >  1:46059/1‑46 (MQ=255)

                                                                                                     aaaaCAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAAttt                                                                                                        >  2:289970/1‑70 (MQ=255)

                                                                                                     aaaaCAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAAttt                                                                                                        <  1:289970/70‑1 (MQ=255)

                                                                                                           gTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGaa                             <  1:1043612/139‑1 (MQ=255)

                                                                                                               ctggcACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg                         >  2:810277/1‑139 (MQ=255)

                                                                                                               ctggcACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg                         <  2:860566/139‑1 (MQ=255)

                                                                                                               aagagacaaGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg                         <  1:221727/131‑1 (MQ=255)

                                                                                                                     cGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGAtt                   <  1:810277/139‑1 (MQ=255)

                                                                                                                                   gCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                               <  1:845760/49‑1 (MQ=255)

                                                                                                                                   gCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                               >  2:845760/1‑49 (MQ=255)

                                                                                                                                   gCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACgg     >  2:325430/1‑139 (MQ=255)

                                                                                                                                    ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                     <  2:304199/58‑1 (MQ=255)

                                                                                                                                    ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                     >  1:304199/1‑58 (MQ=255)

                                                                                                                                    ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgagag              <  1:687273/129‑1 (MQ=255)

                                                                                                                                    ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgagag              >  2:687273/1‑129 (MQ=255)

                                                                                                                                    ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa    >  2:190746/1‑139 (MQ=255)

                                                                                                                                    ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa    >  1:933609/1‑139 (MQ=255)

                                                                                                                                      tgctgcC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGTGGGTCg                                                          >  2:1042881/1‑83 (MQ=255)

                                                                                                                                      tgctgcC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGTGGGTCg                                                          <  1:1042881/83‑1 (MQ=255)

                                                                                                                                      tgctgcC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAt  <  2:160049/139‑1 (MQ=255)

TCACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑C‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAT  >  NC_000913/2240611‑2240882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A