Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I2 R1 766 64.2 3232166 90.9% 2938038 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,240,754 (C)5→6 coding (915/1041 nt) galS ← galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,240,7491.C96.6% 201.0 / ‑0.5 59coding (920/1041 nt)galSgalactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (25/32);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

TCACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATG  >  NC_000913/2240611‑2240873
                                                                                                                                           |                                                                                                                             
tcacAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCctgctg                                                                                                                                <  1:736500/139‑1 (MQ=255)
 cacaGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCtgctg‑c                                                                                                                              >  1:1027465/1‑139 (MQ=255)
  acaGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑cc                                                                                                                             <  2:1273226/139‑1 (MQ=255)
    aGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc‑c                                                                                                                          <  1:1371793/139‑1 (MQ=255)
      aTTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc‑ccc                                                                                                                        >  1:673719/1‑138 (MQ=255)
        tGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc‑ccctg                                                                                                                      <  1:1088655/139‑4 (MQ=255)
        tGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc‑ccctg                                                                                                                      >  1:514613/1‑136 (MQ=255)
                gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCa                                                                                                               >  2:176467/1‑139 (MQ=255)
                     aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCg                                                                                                          <  1:35188/139‑1 (MQ=255)
                         ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTgg                                                                                                      >  2:1363511/1‑139 (MQ=255)
                                   tgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGc                                                                                            <  2:1027465/139‑1 (MQ=255)
                                           tGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg                                                                                    <  1:337849/139‑1 (MQ=255)
                                              tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                 <  2:671568/139‑1 (MQ=255)
                                              tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                 <  1:176467/139‑1 (MQ=255)
                                               cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                      <  1:1392191/133‑1 (MQ=255)
                                               cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                      >  2:1392191/1‑133 (MQ=255)
                                               cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAAt                                                                                >  2:899588/1‑139 (MQ=255)
                                                 ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATgg                                                                              >  2:532691/1‑139 (MQ=255)
                                                          tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                     <  1:532691/139‑1 (MQ=255)
                                                              gCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                      >  1:1253088/1‑118 (MQ=255)
                                                              gCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                      <  2:1253088/118‑1 (MQ=255)
                                                                   ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAg                                                                                                                   >  1:1247550/1‑84 (MQ=255)
                                                                   ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAg                                                                                                                   <  2:1247550/84‑1 (MQ=255)
                                                                    tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAAttt                                                                                               <  2:1120231/103‑1 (MQ=255)
                                                                    tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCCGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAAttt                                                                                               >  1:1120231/1‑103 (MQ=255)
                                                                       gACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTg                                                        <  1:45224/139‑1 (MQ=255)
                                                                         cAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCg                                                      <  2:514613/139‑1 (MQ=255)
                                                                            aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt                                                   <  2:929447/139‑1 (MQ=255)
                                                                            aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt                                                   <  2:1587822/139‑1 (MQ=255)
                                                                            aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCTCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt                                                   >  1:693433/1‑139 (MQ=255)
                                                                             aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc                                                  <  1:84401/139‑1 (MQ=255)
                                                                             aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc                                                  <  1:27047/139‑1 (MQ=255)
                                                                              tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt                                                   >  1:1565358/1‑137 (MQ=255)
                                                                              tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt                                                   <  2:1565358/137‑1 (MQ=255)
                                                                              tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCg                                                 >  2:681829/1‑139 (MQ=255)
                                                                                  gACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGgtgt                                             >  2:1244010/1‑139 (MQ=255)
                                                                                   aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTa                                            <  1:1202721/139‑1 (MQ=255)
                                                                                     gCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAAc                                          >  2:1451375/1‑139 (MQ=255)
                                                                                            cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                   <  1:1244010/139‑1 (MQ=255)
                                                                                            cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCTCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                   <  2:693433/139‑1 (MQ=255)
                                                                                                 gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg                                                                                       <  2:620214/82‑1 (MQ=255)
                                                                                                 gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg                                                                                       >  1:620214/1‑82 (MQ=255)
                                                                                                 gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                     <  2:748875/100‑1 (MQ=255)
                                                                                                 gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                     >  1:748875/1‑100 (MQ=255)
                                                                                                 gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGaa                              >  2:1178367/1‑139 (MQ=255)
                                                                                                   aTAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAata                            <  1:1363511/139‑1 (MQ=255)
                                                                                                            tggctggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCg                                                                                                          <  2:67768/52‑1 (MQ=255)
                                                                                                            tggctggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCg                                                                                                          >  1:67768/1‑52 (MQ=255)
                                                                                                             ggctggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAAc                  <  1:1178367/139‑1 (MQ=255)
                                                                                                                 ggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgat              >  2:295523/1‑139 (MQ=255)
                                                                                                                   cACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGTCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg                                                                   >  2:1187652/1‑84 (MQ=255)
                                                                                                                   cACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGTCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg                                                                   <  1:1187652/84‑1 (MQ=255)
                                                                                                                        ggATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag       <  1:110034/139‑1 (MQ=255)
                                                                                                                             aaTATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATg  >  1:559506/1‑139 (MQ=255)
                                                                                                                                 ttGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                     <  1:550467/68‑1 (MQ=255)
                                                                                                                                 ttGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                     >  2:550467/1‑68 (MQ=255)
                                                                                                                                   gCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                 <  1:326489/54‑1 (MQ=255)
                                                                                                                                   gCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                 >  2:326489/1‑54 (MQ=255)
                                                                                                                                    ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                     <  1:45698/65‑1 (MQ=255)
                                                                                                                                    ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                     >  2:45698/1‑65 (MQ=255)
                                                                                                                                    ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACgg                                        >  1:1068516/1‑94 (MQ=255)
                                                                                                                                    ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACgg                                        <  2:1068516/94‑1 (MQ=255)
                                                                                                                                    ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGaa                    <  2:429484/114‑1 (MQ=255)
                                                                                                                                    ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGaa                    >  1:429484/1‑114 (MQ=255)
                                                                                                                                    ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTga        <  2:700033/126‑1 (MQ=255)
                                                                                                                                    ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTga        >  1:700033/1‑126 (MQ=255)
                                                                                                                                      tgctgcCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg                                                                   <  1:904983/65‑1 (MQ=255)
                                                                                                                                      tgctgcCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg                                                                   >  2:904983/1‑65 (MQ=255)
                                                                                                                                      tgctgcCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag       <  2:690861/125‑1 (MQ=255)
                                                                                                                                      tgctgcCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag       >  1:690861/1‑125 (MQ=255)
                                                                                                                                           |                                                                                                                             
TCACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATG  >  NC_000913/2240611‑2240873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A