Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,240,754	(C)_5→6	coding (915/1041 nt)	galS ←	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,240,749	1	.	C	98.7%	274.3 / ‑3.9	77	coding (920/1041 nt)	galS	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (37/39); total (38/39)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA  >  NC_000913/2240612‑2240880

cacaGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCtgctg‑‑c                                                                                                                                     >  2:1411425/1‑139 (MQ=255)

  caGATTGAAAGCGGTTACATCGCTTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGAGACGCACTAACGTCGGCATAAAACAGTGGCTGGCAAGAGGATCAATATTGCCTGCTG‑‑ccc                                                                                                                                   <  1:1283044/139‑1 (MQ=255)

  caGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑‑ccc                                                                                                                                   <  2:188979/139‑1 (MQ=255)

   aGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑‑ccc‑c                                                                                                                                 <  2:1590885/139‑1 (MQ=255)

   aGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑‑ccc‑c                                                                                                                                 <  1:999096/139‑1 (MQ=255)

   aGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑‑ccc‑c                                                                                                                                 <  2:1462188/139‑1 (MQ=255)

         aaaGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑‑ccc‑ccctgca                                                                                                                           >  1:713488/1‑134 (MQ=255)

           aGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAg                                                                                                                          <  2:1531042/139‑1 (MQ=255)

                ttACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAg                                                                                                                     >  2:942974/1‑139 (MQ=255)

                    aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCg                                                                                                                 <  1:905920/139‑1 (MQ=255)

                    aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCg                                                                                                                 <  1:1411425/139‑1 (MQ=255)

                          tGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCt                                                                                                           >  1:603500/1‑139 (MQ=255)

                            aTTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTaa                                                                                                         >  2:1330082/1‑139 (MQ=255)

                                gttgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTc                                                                                                     <  2:713488/139‑1 (MQ=255)

                                  tgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGc                                                                                                   <  1:177339/139‑1 (MQ=255)

                                      ttAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAtt                                                                                               <  2:929380/139‑1 (MQ=255)

                                       tAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg                                                                                              >  1:321608/1‑139 (MQ=255)

                                        aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTgg                                                                                                             <  2:692705/123‑1 (MQ=255)

                                        aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTgg                                                                                                             >  1:692705/1‑123 (MQ=255)

                                           gATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGc                                                                                          >  2:364199/1‑139 (MQ=255)

                                              cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAAt                                                                                       <  2:525060/139‑1 (MQ=255)

                                               gccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCa                                                                                         >  2:634822/1‑136 (MQ=255)

                                               gccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCa                                                                                         <  1:634822/136‑1 (MQ=255)

                                               gccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATg                                                                                      >  2:891356/1‑139 (MQ=255)

                                               gccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATg                                                                                      >  2:1232678/1‑139 (MQ=255)

                                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATgg                                                                                     >  2:1485676/1‑139 (MQ=255)

                                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATgg                                                                                     >  1:143870/1‑139 (MQ=255)

                                                    cgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATCTTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATa                                                                                 >  1:1034935/1‑139 (MQ=255)

                                                        ttCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGc                                                                             >  1:736511/1‑139 (MQ=255)

                                                         tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  2:727888/139‑1 (MQ=255)

                                                         tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  1:1330082/139‑1 (MQ=255)

                                                         tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  2:143870/139‑1 (MQ=255)

                                                         tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  2:772733/139‑1 (MQ=255)

                                                         tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  1:15393/139‑1 (MQ=255)

                                                                tgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGt                                                                     >  1:364819/1‑139 (MQ=255)

                                                                  ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTa                                                                   >  2:376291/1‑139 (MQ=255)

                                                                  ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTa                                                                   >  2:1294879/1‑139 (MQ=255)

                                                                       aCAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGc                                                              >  2:807725/1‑139 (MQ=255)

                                                                          gAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCggg                                                           <  1:1232678/139‑1 (MQ=255)

                                                                           aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt                                                          <  1:1294879/139‑1 (MQ=255)

                                                                           aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt                                                          <  1:807725/139‑1 (MQ=255)

                                                                            aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            >  2:58338/1‑120 (MQ=255)

                                                                            aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  1:58338/120‑1 (MQ=255)

                                                                            aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc                                                         <  2:321608/139‑1 (MQ=255)

                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg                                                                                              >  1:538509/1‑96 (MQ=255)

                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg                                                                                              <  2:538509/96‑1 (MQ=255)

                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTa                                                   >  2:526946/1‑139 (MQ=255)

                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑‑ccc                                                                                                                                   <  1:476018/59‑1 (MQ=255)

                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑‑ccc                                                                                                                                   >  2:476018/1‑59 (MQ=255)

                                                                                     cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACg                                                >  2:1418830/1‑139 (MQ=255)

                                                                                           cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                          <  2:1034935/139‑1 (MQ=255)

                                                                                               ggCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGa                                      >  2:621001/1‑139 (MQ=255)

                                                                                                         aGTGGCTGGCACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGa                            <  2:1394066/139‑1 (MQ=255)

                                                                                                           tggctggcACGAGGATCAATATTGCCTGCTGCCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAt                                                                                                <  2:969230/70‑1 (MQ=255)

                                                                                                           tggctggcACGAGGATCAATATTGCCTGCTGCCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAt                                                                                                >  1:969230/1‑70 (MQ=255)

                                                                                                            ggctggcACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAAc                         <  2:364819/139‑1 (MQ=255)

                                                                                                             ataagagacaGGATCAATATTGCCGGCTGC‑CCC‑CCTGCAAGGCTAGTTCGATGGCTAATTTCGCCATTGAAGCAAGGGGATAGCGCACGGTCGTTAATTGCGGGTCGATGTAGCGGGCGATGGGAATATCATCGAAAcc                        <  2:1537479/130‑1 (MQ=255)

                                                                                                              ctggcACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg                       <  2:603500/139‑1 (MQ=255)

                                                                                                               tggcACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                      <  1:482276/139‑1 (MQ=255)

                                                                                                                ggcACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGaa                                                                                            >  1:1441159/1‑68 (MQ=255)

                                                                                                                ggcACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGaa                                                                                            <  2:277936/68‑1 (MQ=255)

                                                                                                                ggcACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGaa                                                                                            >  1:277936/1‑68 (MQ=255)

                                                                                                                ggcACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGaa                                                                                            <  2:1441159/68‑1 (MQ=255)

                                                                                                                ggcACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGa                                           >  1:676757/1‑117 (MQ=255)

                                                                                                                ggcACGAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGa                                           <  2:676757/117‑1 (MQ=255)

                                                                                                                     gAGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTg                <  1:364199/139‑1 (MQ=255)

                                                                                                                      aGGATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTga               <  1:526946/139‑1 (MQ=255)

                                                                                                                        gATCAATATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgaga             >  1:1589870/1‑139 (MQ=255)

                                                                                                                            aaTATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  1:320667/72‑1 (MQ=255)

                                                                                                                            aaTATTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            >  2:320667/1‑72 (MQ=255)

                                                                                                                               atTGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa      <  1:942974/139‑1 (MQ=255)

                                                                                                                                ttGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAAc     >  2:1408738/1‑139 (MQ=255)

                                                                                                                                 tGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCt                                                                                                           >  2:726794/1‑36 (MQ=255)

                                                                                                                                 tGCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCt                                                                                                           <  1:726794/36‑1 (MQ=255)

                                                                                                                                  gCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTc                                                                                                     <  2:1195695/41‑1 (MQ=255)

                                                                                                                                  gCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTc                                                                                                     >  1:1195695/1‑41 (MQ=255)

                                                                                                                                  gCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                   <  1:1151025/59‑1 (MQ=255)

                                                                                                                                  gCCTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa                                                                                   >  2:1151025/1‑59 (MQ=255)

                                                                                                                                   tctgctgc‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa      <  2:1465033/134‑1 (MQ=255)

                                                                                                                                   tctgctgc‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa      >  1:1465033/2‑135 (MQ=255)

                                                                                                                                   ccTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                        <  2:148812/53‑1 (MQ=255)

                                                                                                                                   ccTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                        >  1:980926/1‑53 (MQ=255)

                                                                                                                                   ccTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                        >  1:148812/1‑53 (MQ=255)

                                                                                                                                   ccTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                        <  2:980926/53‑1 (MQ=255)

                                                                                                                                   ccTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGTGATGTAACGGa  >  1:1498449/1‑139 (MQ=255)

                                                                                                                                   ccTGCTGC‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa  >  1:1158578/1‑139 (MQ=255)

                                                                                                                                   ccTGCTGC‑CCC‑CCTGCAAGGACAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa  >  1:946467/1‑139 (MQ=255)

                                                                                                                                   ccTGCTG‑‑CCCTCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGCAACCGATGATTgagactgtctcttat  >  1:1537479/1‑128 (MQ=255)

                                                                                                                                     tgctgc‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                             >  2:1493391/1‑46 (MQ=255)

                                                                                                                                     tgctgc‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                             <  1:1493391/46‑1 (MQ=255)

                                                                                                                                     tgctgc‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGAtt                 >  2:521438/1‑122 (MQ=255)

                                                                                                                                     tgctgc‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGAtt                 <  1:521438/122‑1 (MQ=255)

CACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA  >  NC_000913/2240612‑2240880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A