Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,240,754 |
(C)5→6 |
coding (915/1041 nt) |
galS ← |
galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,240,749 | 1 | . | C | 93.9%
| 108.2
/ 0.1
| 33 | coding (920/1041 nt) | galS | galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (12/19); total (13/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
AGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑CCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA > NC_000913/2240615‑2240880
|
aGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccc < 1:381210/139‑1 (MQ=255)
aTTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccc > 1:60359/1‑138 (MQ=255)
gAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc < 2:729907/139‑5 (MQ=255)
cGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACTAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGc > 1:714494/1‑139 (MQ=255)
gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCa > 2:966667/1‑139 (MQ=255)
ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTgg > 1:845694/1‑139 (MQ=255)
ttAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAtt < 2:822055/139‑1 (MQ=255)
ttAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAtt > 2:118157/1‑139 (MQ=255)
ttAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAtt > 1:1013750/1‑139 (MQ=255)
tAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg > 1:1062409/1‑139 (MQ=255)
aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa < 2:395603/139‑1 (MQ=255)
tGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg < 2:270477/139‑1 (MQ=255)
tGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg < 2:541756/139‑1 (MQ=255)
tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa < 2:845694/139‑1 (MQ=255)
tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa < 2:1062409/139‑1 (MQ=255)
cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAAt < 2:245446/139‑1 (MQ=255)
cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAAt < 2:817168/139‑1 (MQ=255)
gccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa < 2:1013750/139‑1 (MQ=255)
gccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGa < 1:976696/139‑1 (MQ=255)
tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 2:1108482/139‑1 (MQ=255)
tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:809101/139‑1 (MQ=255)
tgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc > 2:296289/1‑79 (MQ=255)
tgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑cccccctgc < 1:296289/83‑5 (MQ=255)
aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt > 2:1108187/1‑139 (MQ=255)
aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTa > 2:223624/1‑139 (MQ=255)
tAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc < 1:223624/139‑1 (MQ=255)
tggctggcACGAGGATCAATATTGCCTGCTAC‑CCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa > 2:774214/1‑72 (MQ=255)
tggctggcACGAGGATCAATATTGCCTGCTAC‑CCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa < 1:774214/72‑1 (MQ=255)
ggctggcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAAc < 2:290743/139‑1 (MQ=255)
gcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc < 2:1014016/102‑1 (MQ=255)
gcACGAGGATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc > 1:1014016/1‑102 (MQ=255)
ggATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag < 2:60359/139‑1 (MQ=255)
ggATCAATATTGCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag < 1:1108187/139‑1 (MQ=255)
atcgCCTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGCGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa < 2:498245/136‑1 (MQ=255)
ccTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa > 1:779777/1‑48 (MQ=255)
ccTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa < 2:779777/48‑1 (MQ=255)
ccTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc < 1:85281/96‑1 (MQ=255)
ccTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc > 2:85281/1‑96 (MQ=255)
ccTGCT‑GCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa > 1:966066/1‑139 (MQ=255)
|
AGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCT‑G‑CCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA > NC_000913/2240615‑2240880
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
