Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,268,487	(T)_7→8	intergenic (+180/+322)	tyrB → / ← yjbS	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,268,480	1	.	T	93.3%	88.1 / ‑0.3	30	intergenic (+173/+329)	tyrB/yjbS	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent/uncharacterized protein
Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (13/15); total (13/17)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTAAACTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCTGTGACTTTCTGA  >  NC_000913/4268346‑4268613

aaTTAAACTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑‑tttt                                                                                                                                   >  2:452086/1‑139 (MQ=255)

       cTGTCGTCGCTTTCTCTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACCTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑‑ttttttttggg                                                                                                                            >  1:933002/1‑135 (MQ=255)

       cTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑‑TTTTTTTtggg                                                                                                                            <  1:1194538/139‑2 (MQ=255)

       cTGTCGTCCCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑‑ttttttttggg                                                                                                                            <  2:933002/139‑5 (MQ=255)

           cgtcgCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGa                                                                                                                         <  1:340673/139‑1 (MQ=255)

                  ttCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGtaataa                                                                                                                  >  1:924597/1‑139 (MQ=255)

                         tttctttATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCtac                                                                                                           <  1:452086/139‑3 (MQ=255)

                               tataGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCt                                                                                                     <  2:213044/139‑1 (MQ=255)

                               tataGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCt                                                                                                     <  2:1392571/139‑1 (MQ=255)

                                     tgatTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCat                                                                                               >  1:346050/1‑139 (MQ=255)

                                       atTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑‑ttttttttggg                                                                                                                            >  2:317068/1‑103 (MQ=255)

                                       atTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑‑TTTTTTTtggg                                                                                                                            <  1:317068/107‑2 (MQ=255)

                                              tGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAAt                                                                                       <  2:472138/139‑1 (MQ=255)

                                                              cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCgg                                                                      >  2:110861/1‑139 (MQ=255)

                                                               gTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCggg                                                                     <  2:346050/139‑1 (MQ=255)

                                                                             aCGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTCTCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGa                                                       <  2:521779/139‑1 (MQ=255)

                                                                                  gttAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGc                                                                                                           >  1:163325/1‑82 (MQ=255)

                                                                                  gttAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGc                                                                                                           <  2:163325/82‑1 (MQ=255)

                                                                                           tttATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAata                                                    <  1:875237/128‑1 (MQ=255)

                                                                                           tttATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAata                                                    >  2:875237/1‑128 (MQ=255)

                                                                                            ttATTTTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCt                                        <  1:1459878/139‑1 (MQ=255)

                                                                                            ttATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCt                                        >  1:1462505/1‑139 (MQ=255)

                                                                                                         tATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTg                                                                                                            >  2:1168938/1‑58 (MQ=255)

                                                                                                         tATCCCAATTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTg                                                                                                            <  1:1168938/58‑1 (MQ=255)

                                                                                                               aaTTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTtaataat                                                                               <  2:183633/81‑1 (MQ=255)

                                                                                                               aaTTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTtaataat                                                                               >  1:183633/1‑81 (MQ=255)

                                                                                                                aTTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTg                                                                                                            <  2:437351/51‑1 (MQ=255)

                                                                                                                aTTATCTGGAATTCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTg                                                                                                            >  1:437351/1‑51 (MQ=255)

                                                                                                                           ttCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGt                                                                >  1:473401/1‑84 (MQ=255)

                                                                                                                           ttCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGt                                                                <  2:473401/84‑1 (MQ=255)

                                                                                                                           ttCCTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCTGTGAc         >  2:1114266/1‑139 (MQ=255)

                                                                                                                              cTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTg                                                                                                            <  2:156354/37‑1 (MQ=255)

                                                                                                                              cTTATCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTg                                                                                                            >  1:156354/1‑37 (MQ=255)

                                                                                                                                  tCCTGT‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCTGTGACTTTCTga  >  1:1320526/1‑139 (MQ=255)

AATTAAACTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCTGTGACTTTCTGA  >  NC_000913/4268346‑4268613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A