Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,268,487	(T)_7→8	intergenic (+180/+322)	tyrB → / ← yjbS	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,268,480	1	.	T	96.9%	97.6 / ‑3.3	32	intergenic (+173/+329)	tyrB/yjbS	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent/uncharacterized protein
Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (17/14); total (17/15)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCT  >  NC_000913/4268353‑4268601

cTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑TTTTTTTtggg                                                                                                                <  2:537880/139‑2 (MQ=255)

                  tttctttATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGc                                                                                               <  1:379484/139‑1 (MQ=255)

                    tctttATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTaataat                                                                                                     <  2:720615/131‑1 (MQ=255)

                    tctttATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTaataat                                                                                                     >  1:720615/1‑131 (MQ=255)

                    tctttATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGcac                                                                                             >  2:53640/1‑139 (MQ=255)

                            gatgatTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAAcc                                                                                     >  1:246327/1‑139 (MQ=255)

                            gatgatTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAAcc                                                                                     >  1:308576/1‑139 (MQ=255)

                               gatTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTg                                                                                                               >  2:611692/1‑110 (MQ=255)

                               gatTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTg                                                                                                               <  1:611692/110‑1 (MQ=255)

                                         aTGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTta                                                                        <  2:570701/139‑1 (MQ=255)

                                         aTGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTta                                                                        <  2:623163/139‑1 (MQ=255)

                                                    cTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTAt                                                             <  2:246327/139‑1 (MQ=255)

                                                       cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATc                                                                                          >  1:636064/1‑107 (MQ=255)

                                                       cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATc                                                                                          <  2:636064/107‑1 (MQ=255)

                                                       cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAAGTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCgg                                                          >  1:522000/1‑139 (MQ=255)

                                                       cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑ttttttttggg                                                                                                                <  2:181976/84‑5 (MQ=255)

                                                       cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑ttttttttggg                                                                                                                >  1:181976/1‑80 (MQ=255)

                                                          gagagaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                                <  1:456112/98‑1 (MQ=255)

                                                          gagagaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                                >  2:456112/1‑98 (MQ=255)

                                                             agaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                                <  2:545234/95‑1 (MQ=255)

                                                             agaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                                >  1:545234/1‑95 (MQ=255)

                                                                 aataaACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACg                                                >  1:723127/1‑139 (MQ=255)

                                                                      aCGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTg                                                                                                               >  2:499132/1‑71 (MQ=255)

                                                                      aCGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTg                                                                                                               <  1:499132/71‑1 (MQ=255)

                                                                        gttgttAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTtaataat                                                                   <  1:600747/113‑1 (MQ=255)

                                                                        gttgttAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTtaataat                                                                   >  2:600747/1‑113 (MQ=255)

                                                                              aGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCt                                   <  2:672977/139‑1 (MQ=255)

                                                                                                   aTCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCttt              >  1:76130/1‑139 (MQ=255)

                                                                                                   aTCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCttt              >  2:549086/1‑139 (MQ=255)

                                                                                                    tCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCt                <  2:321647/136‑1 (MQ=255)

                                                                                                    tCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCt                >  1:321647/1‑136 (MQ=255)

                                                                                                         aTTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTa        >  1:56332/1‑139 (MQ=255)

                                                                                                               tGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCt  >  1:801955/1‑139 (MQ=255)

                                                                                                               tGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCt  <  2:801955/139‑1 (MQ=255)

CTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCT  >  NC_000913/4268353‑4268601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A