Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,268,487	(T)_7→8	intergenic (+180/+322)	tyrB → / ← yjbS	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,268,480	1	.	T	100.0%	125.3 / NA	40	intergenic (+173/+329)	tyrB/yjbS	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent/uncharacterized protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (20/20); total (20/20)

AACTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTA  >  NC_000913/4268351‑4268595

aaCTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑ttttttttg                                                                                                            >  1:1280942/1‑137 (MQ=255)

  cTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑ttttttttggg                                                                                                          <  1:626108/139‑5 (MQ=255)

      cgtcgCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGa                                                                                                       >  2:525679/1‑139 (MQ=255)

                   ctttctttATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          <  1:1519068/139‑1 (MQ=255)

                   ctttctttATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          <  2:1280942/139‑1 (MQ=255)

                   ctttctttATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          <  1:525679/139‑1 (MQ=255)

                                              ccATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTaataat                                                                                               >  2:42652/1‑107 (MQ=255)

                                              ccATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTaataat                                                                                               <  1:42652/107‑1 (MQ=255)

                                                  cGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTtaataat                                                             <  2:202837/137‑1 (MQ=255)

                                                  cGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTtaataat                                                             >  1:202837/1‑137 (MQ=255)

                                                         cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGtaat                                                                                                  <  2:866068/93‑1 (MQ=255)

                                                         cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGtaat                                                                                                  >  1:866068/1‑93 (MQ=255)

                                                         cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          >  2:802634/1‑101 (MQ=255)

                                                         cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          <  1:802634/101‑1 (MQ=255)

                                                         cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAAtt                                                                    <  1:540876/123‑1 (MQ=255)

                                                         cGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAAtt                                                                    >  2:540876/1‑123 (MQ=255)

                                                             agagaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTg                                                                                                         <  2:685340/82‑1 (MQ=255)

                                                             agagaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTg                                                                                                         >  1:685340/1‑82 (MQ=255)

                                                             agagaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTtaataat                                                             >  1:1614909/1‑126 (MQ=255)

                                                             agagaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTtaataat                                                             <  2:1614909/126‑1 (MQ=255)

                                                             agagaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGttt                                                >  2:677869/1‑139 (MQ=255)

                                                               agaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          <  1:11481/95‑1 (MQ=255)

                                                               agaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          >  2:11481/1‑95 (MQ=255)

                                                               agaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAAc                                                                                >  1:441071/1‑105 (MQ=255)

                                                               agaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAAc                                                                                <  2:441071/105‑1 (MQ=255)

                                                               agaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGt                                              >  1:282620/1‑139 (MQ=255)

                                                                   aataaACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAAttt                                                                                             >  2:1547183/1‑88 (MQ=255)

                                                                   aataaACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAAttt                                                                                             <  1:1547183/88‑1 (MQ=255)

                                                                    ataaACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGa                                         <  2:282620/139‑1 (MQ=255)

                                                                           ttgttAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGc                                                                                         <  2:677788/84‑1 (MQ=255)

                                                                           ttgttAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGc                                                                                         >  1:677788/1‑84 (MQ=255)

                                                                                     ttttATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATc                        >  2:1404748/1‑139 (MQ=255)

                                                                                               acgCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGtaat                                                                                                  >  2:186937/3‑55 (MQ=255)

                                                                                               aaGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGtaat                                                                                                  <  1:186937/55‑1 (MQ=255)

                                                                                                     aTCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          >  1:1575408/1‑57 (MQ=255)

                                                                                                     aTCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          <  2:1575408/57‑1 (MQ=255)

                                                                                                       cccAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          >  2:64872/1‑55 (MQ=255)

                                                                                                       cccAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTg                                                                                          <  1:64872/55‑1 (MQ=255)

                                                                                                                 tGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAAc                                                                                <  2:433739/55‑1 (MQ=255)

                                                                                                                 tGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAAc                                                                                >  1:433739/1‑55 (MQ=255)

                                                                                                                         cTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTa  <  1:457285/125‑1 (MQ=255)

                                                                                                                         cTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTa  >  2:457285/1‑125 (MQ=255)

AACTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTA  >  NC_000913/4268351‑4268595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A