Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F20 I2 R1
|
11 |
30.6 |
1075110 |
91.5% |
983725 |
145.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_bme_pgi |
3,486,574 |
C→A |
A152E (GCA→GAA) |
crp → |
cAMP‑activated global transcription factor, mediator of catabolite repression |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_bme_pgi | 3,486,574 | 0 | C | A | 96.5%
| 80.5
/ NA
| 29 | A152E (GCA→GAA) | crp | cAMP‑activated global transcription factor, mediator of catabolite repression |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (18/10); total (19/10) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACAC > NC_000913_3_bme_pgi/3486430‑3486719
|
gCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCCCGGACTCTGCTGCATCTTGAaaaa > 2:182394/1‑149 (MQ=255)
agtGATTCATGGAAACGCGGACATTCGGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAaacaac < 1:304702/147‑1 (MQ=255)
ttGATTCAGTTAAACCCGCACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAAcc < 1:90661/149‑1 (MQ=255)
ttCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGAc > 2:280204/1‑149 (MQ=255)
ggACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGCGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAACCTGGAAAAACAACCCGACGCCATGACTCAcc > 1:127647/1‑149 (MQ=255)
tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCcaa > 1:335606/1‑149 (MQ=255)
ttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGACTGCAAAt > 1:273805/1‑149 (MQ=255)
tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGAAGATCaa > 1:514995/1‑149 (MQ=255)
gaggtGGCGGGTCGACTGCAAGTCACTTCAGACAAAGCGGGCAACCTGGCCTTCCTCGACCTCACGGGCCGCCTCGCACAGTCCCTGCTCAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTAc < 2:342708/145‑1 (MQ=255)
tGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTCCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGt < 2:476531/149‑1 (MQ=255)
gcgcGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa > 1:389582/1‑149 (MQ=255)
gcgcGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa > 1:478080/1‑149 (MQ=255)
gcgcGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa > 2:147600/1‑149 (MQ=255)
gcgTCGTCTGCGCGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCTCATTGCACAGACTCTGCTGAAGCTGAAAAAACAACCAGACGCTATGCCTCACCCGGCCGGGATGCAAATCAACATATCCCGTCAgt > 2:35498/1‑148 (MQ=255)
tcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg < 1:305220/149‑1 (MQ=255)
tcTGCAAGTCAAGTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGGGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGTCGGTATGCAAATCAACATTACCCGTCAGGAAATTg < 2:454718/149‑1 (MQ=255)
tGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCAGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATAACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt > 2:528255/1‑149 (MQ=255)
aaGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGTGTCACCCATTCGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAg > 1:524978/1‑149 (MQ=255)
cACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAAGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTg < 1:437913/149‑1 (MQ=255)
aCTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATGTGGCAAAACAACCAGACGCTATAAGTCACCCGGTCGGTATGCAAATCAAAATTACCCGTCAGGGAATTGGGCCGATTGt > 1:149777/1‑149 (MQ=255)
agagAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCCAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt < 2:128450/149‑1 (MQ=255)
agagAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt < 1:280204/149‑1 (MQ=255)
gCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAACCAACCAGACGCTATGACTCACCCGGCCGGTATGCCAATCAAAATTACCCGTCAGGAAATTGCTCAGATTATCGCCTGTTTTCGTGaa > 1:262195/1‑149 (MQ=255)
gACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt > 2:106834/1‑149 (MQ=255)
tGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAg > 2:36547/1‑149 (MQ=255)
gggACGGATTGCACAGTGTCTGCCGTATGGTGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGc < 2:268583/149‑1 (MQ=255)
tgctgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGACAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGCTCATAACCTGAta > 2:445584/1‑148 (MQ=255)
tgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTcc > 1:223228/1‑149 (MQ=255)
tGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAGATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTGTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCacac > 2:340797/1‑149 (MQ=255)
|
GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACAC > NC_000913_3_bme_pgi/3486430‑3486719
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
