Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F7 I2 R1
|
12 |
31.6 |
1093458 |
93.0% |
1016915 |
144.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_bme_pgi |
3,486,574 |
C→A |
A152E (GCA→GAA) |
crp → |
cAMP‑activated global transcription factor, mediator of catabolite repression |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_bme_pgi | 3,486,574 | 0 | C | A | 97.2%
| 114.7
/ NA
| 36 | A152E (GCA→GAA) | crp | cAMP‑activated global transcription factor, mediator of catabolite repression |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (18/17); total (18/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACAC > NC_000913_3_bme_pgi/3486432‑3486719
|
cAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAaaca > 1:398820/1‑149 (MQ=255)
aTTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCACAGAAAGGGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAaacaac < 1:348958/149‑1 (MQ=255)
aTTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGACGCGTCGTCTGCACGTCACTTCAGGGAAAGTGGGCAACCTGGCGTGCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAaaaaacac > 2:227514/1‑145 (MQ=255)
cAGGTAAACCCGGACATTCGGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGc < 1:530021/149‑1 (MQ=255)
aGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCt > 2:18938/1‑149 (MQ=255)
gTAAACCCGGACATTCTGATGCGTTTTTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCACCCCGGCGTTCCTCGACGTGACGGGTCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTAt < 1:382849/149‑1 (MQ=255)
gTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGCCGCTAt > 2:213301/1‑149 (MQ=255)
aaaCCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGa < 1:18938/149‑1 (MQ=255)
ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCa > 2:483359/1‑149 (MQ=255)
tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCaaa > 1:191947/1‑149 (MQ=255)
tGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATc < 2:328568/149‑1 (MQ=255)
tGTCGGCGCAGATGGCGCGGCGTCGGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATc < 2:462872/149‑1 (MQ=255)
gtcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAAACAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTAGAGAAAtt > 2:369959/1‑149 (MQ=255)
tcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg < 2:217000/149‑1 (MQ=255)
tGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGACAGGAAATTgat > 1:222703/1‑147 (MQ=255)
cAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGACCGGTATGCAAGTCAAAATTACCCGCCGGGAAATTGGtaa > 2:162069/1‑147 (MQ=255)
gTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATg < 1:71955/96‑1 (MQ=255)
gTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATg > 2:71955/1‑96 (MQ=255)
tCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg > 2:148225/1‑149 (MQ=255)
tCAGACAGAGTTGGCAACCTGTCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg < 2:209416/149‑1 (MQ=255)
cAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGc < 1:384494/149‑1 (MQ=255)
tGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGt < 1:148225/149‑1 (MQ=255)
gCGGTCCTCGACGTGAGGAGCCGCAATGCACAGACTCTGCTGAATCTGGAAAAGCAACAAGACGCTATAAGTCACCCGGACGGTAATCAAATCAAAATTAGCAATAAGGAACGTGGATCGAGTGTCGGCTGGTCAGCTAAAACCGgggg > 2:221981/1‑149 (MQ=255)
gggCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGc < 1:528627/149‑1 (MQ=255)
cGCATTGCACAGACTCTGCTTAATCTGGAAAAACAACCCGACGCGATGACCCACACCGACGGAAGGCAAATCAAAATTACCCGTCAGCAAATTATTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTATGAAGATGCTGGa > 2:199396/1‑149 (MQ=255)
ggCACAGACGCTGATGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc < 1:369959/148‑1 (MQ=255)
cacaGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTATTGTGTGTTCTGGGGATGCATTCTGAAGATGCTGGAAGATcgg > 1:423813/1‑147 (MQ=255)
acatcctctGCTGTATCGTGCAAAACACCCAGCCGCTACGACTCACCCGGACGGTATGGAAATCAAAATTACCCGTCATGAAATTGGTTAGAGTGACGGCTGTTCTCGTGAAACTGTGGGTCGCATTCTGAAGATGCTGGAAGATCAGa < 2:463390/144‑1 (MQ=255)
gACTCTGCTGAATCTGGAAAAACACCCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAcc < 1:430043/149‑1 (MQ=255)
tctGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAcataa > 1:36863/1‑145 (MQ=255)
tctGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGa < 1:410593/149‑1 (MQ=255)
tctGCTGAATCTGGAAAAACAACCAGACGATATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGa < 1:337213/149‑1 (MQ=255)
tgctgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtc > 2:464542/1‑149 (MQ=255)
gctgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtct > 1:526897/1‑149 (MQ=255)
tgAATCTGGAAAAACAATCAGAGGCTATGGCGAACCTGGACGGTAGGCAAATCAAAATTACGCGTGAGGAAATTGGTGAGATTGTCGGCTGTTCGCGGGAAACCGTGGGTCGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTcc < 2:516473/149‑1 (MQ=255)
tGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGCTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCacac > 1:486411/1‑149 (MQ=255)
|
CAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACAC > NC_000913_3_bme_pgi/3486432‑3486719
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
