Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F45 I2 R1	10	30.1	1054246	93.1%	981503	143.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	3,486,574	C→A	A152E (GCA→GAA)	crp →	cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	3,486,574	0	C	A	100.0%	107.3 / NA	36	A152E (GCA→GAA)	crp	cAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (18/18); total (18/18)

GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCAC  >  NC_000913_3_bme_pgi/3486430‑3486717

gctaaTTGGTTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGACACTTCACAGAATGTGGGCAACCTGGCGTTCCTCGACGTGACCGGCCGCATTGCACAGACTCTGCTGAATCTGGAaaaa                                                                                                                                             <  2:437051/146‑1 (MQ=255)

  cAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAaaca                                                                                                                                           >  2:109629/1‑149 (MQ=255)

    aTTGATTCAGGTAAACCCGCACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGCATCTGGAAAaacaac                                                                                                                                         >  2:35497/1‑149 (MQ=255)

        aTTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGa                                                                                                                                     <  1:109629/149‑1 (MQ=255)

         ttCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCAGAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAgca                                                                                                                                    >  2:462933/1‑147 (MQ=255)

              gTAAACCCGGACATTCTGATGCGTTTCTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAGGTGGGCCACCTGGCGTTCCTCGACGTCACGGACCGCCTTCCACAGACCCTGCTGAATCTGCAACAACAACACGACGCtct                                                                                                                               >  2:233769/1‑147 (MQ=255)

                      ggACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCAcc                                                                                                                       <  1:263211/149‑1 (MQ=255)

                       gACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCAccc                                                                                                                      <  2:287134/149‑1 (MQ=255)

                          aTTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATTACTCACCCGGa                                                                                                                   >  2:230638/1‑149 (MQ=255)

                              tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGt                                                                                                               <  2:149838/149‑1 (MQ=255)

                              tGATGCGTTTGGTTGGACAGGTGGCGCGTCGTCTGCAAGGCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGt                                                                                                               <  1:245170/149‑1 (MQ=255)

                                     tttaacTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCaaa                                                                                                        >  1:523030/6‑149 (MQ=255)

                                         tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaa                                                                                                    <  1:324992/149‑1 (MQ=255)

                                           tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaaaa                                                                                                  <  2:295168/149‑1 (MQ=255)

                                                     gcgcGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa                                                                                        <  1:76534/149‑1 (MQ=255)

                                                              cTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTAt                                                                                                             <  2:39328/119‑1 (MQ=255)

                                                              cTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTAt                                                                                                             >  1:39328/1‑119 (MQ=255)

                                                               tGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTTGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                                                                              >  1:141242/1‑149 (MQ=255)

                                                                  aaGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGCCAGGAAATTTGGCAg                                                                           >  1:311825/1‑149 (MQ=255)

                                                                       gattCACAGAAAGTTGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCGGCTGAATCTGGACAAACAACCAGCCGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt                                                                      <  1:230638/147‑1 (MQ=255)

                                                                             gagaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTg                                                                >  1:308666/1‑149 (MQ=255)

                                                                                                tgTTCCTGGGCGTGGCGGGACGCATTGGAGAGATTGTGCTGGATCTGGAAAACCAATCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTTAGATTGTCGGCTGTTCTCGTGAAACCGTGGGa                                             <  2:251201/148‑1 (MQ=255)

                                                                                                      tCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAtt                                       >  2:443582/1‑149 (MQ=255)

                                                                                                      tCGACGTGACGGGCCGCATTGAACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTAACCAGGACGGTATGCAAATGAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGAGACGCAtt                                       >  1:468240/1‑149 (MQ=255)

                                                                                                              aCGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGAAGCATTCtaacaat                               >  2:396657/1‑143 (MQ=255)

                                                                                                                gggCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCCTTCTGAAGAttc                             >  1:90029/1‑147 (MQ=255)

                                                                                                                 ggCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCt                            <  1:160288/149‑1 (MQ=255)

                                                                                                                  gCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                           <  1:220170/149‑1 (MQ=255)

                                                                                                                  gCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGGCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                           >  2:479355/1‑149 (MQ=255)

                                                                                                                       ctggCACAGCCTCTGCGCAATCTGTAAAAACAACCAGTCGCTATGACTCACCCGGACGGTATGCAAATCAAAATTCCCCGTCAGGAAATTGTTCAGATTGTCGGCTGTTCTCGTGCAACCGTGGGACTCATTCTGAAGATGCTGGAAGa                      <  1:208647/146‑1 (MQ=255)

                                                                                                                         tGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                    <  1:439253/149‑1 (MQ=255)

                                                                                                                                    tgctgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAcc              <  2:201002/144‑1 (MQ=255)

                                                                                                                                    tgctgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAcc              >  1:201002/1‑144 (MQ=255)

                                                                                                                                    tgctgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtc         >  2:289702/1‑149 (MQ=255)

                                                                                                                                      cggAATCTGGAACAACCACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtctc       <  1:525139/147‑1 (MQ=255)

                                                                                                                                           tCTGGAAAAACAACCAGACGCGATGACGCGCCCGGACGGGAGGCAAATCAAAATTACCCGGCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTGAGcac  >  2:261099/1‑149 (MQ=255)

GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCAC  >  NC_000913_3_bme_pgi/3486430‑3486717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A