Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F45 I1 R1	12	36.2	1274254	93.7%	1193975	141.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	3,486,574	C→A	A152E (GCA→GAA)	crp →	cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	3,486,574	0	C	A	100.0%	69.7 / NA	26	A152E (GCA→GAA)	crp	cAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (12/14); total (12/14)

GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGA  >  NC_000913_3_bme_pgi/3486430‑3486697

gCCAATTGATTCAGGTACACCCAGACACTCTGACGCGTTAGTCTACACAGAAGCCGCGTCGTCTACAAGTCACCTCAGAGAAAGTGGGCAACCTGCCGTTCCTCGACGTGACCGGCCGCATTGCACAGACTCTGCTGAATCTGGAaaaa                                                                                                                         >  2:290131/1‑149 (MQ=255)

            aGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCGCAGACTCTGCTGAATCTGGAAAAACAACCAGACGCt                                                                                                             >  2:148631/1‑149 (MQ=255)

            aGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCt                                                                                                             >  2:483143/1‑149 (MQ=255)

              gTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTAt                                                                                                           <  2:385565/149‑1 (MQ=255)

                       gACGTTCTGATGCGGTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCAccc                                                                                                  <  2:115604/149‑1 (MQ=255)

                              tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGt                                                                                           <  1:173763/149‑1 (MQ=255)

                               gATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCATAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTa                                                                                          <  1:501784/149‑1 (MQ=255)

                                     tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACCGTATGCaaa                                                                                    >  2:461214/1‑149 (MQ=255)

                                           tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGTCTCGCCCGGACGATATGCAAATCaaaa                                                                              >  1:194791/1‑149 (MQ=255)

                                               caGATGGGGCCTCGTCTGCAAGTCACTTCCGAGAAGTTGGCCAACCTGGCGTTCGTTGACGTGACGGTCCGCATTGCACAGACTCTGCTTATTCTTTAAAAACAACCAGTCGCTATGACTCTCCCGGACTTTATGCAAATCAAAATTAc                                                                          <  2:121983/149‑1 (MQ=255)

                                                          tcgtcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGCATCTGGAAAAACAACCAGACGCTATGAATCACCCGGACGGTGTGCAAATCAAAATTACCAGTCAGGaaa                                                               >  1:451955/1‑149 (MQ=255)

                                                                       cctgcAGAGGAAGGGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACGCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt                                                  <  2:424661/145‑1 (MQ=255)

                                                                         ctcctagaAAGTCGGCAACCGGTCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTAGGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCg                                                <  1:592030/144‑1 (MQ=255)

                                                                            agagAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                             >  2:402337/1‑149 (MQ=255)

                                                                                  aGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAGTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                       <  2:490990/149‑1 (MQ=255)

                                                                                           ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                             <  2:263252/134‑1 (MQ=255)

                                                                                           ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                             >  1:263252/1‑134 (MQ=255)

                                                                                            cTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGt                             <  1:334003/149‑1 (MQ=255)

                                                                                                  ttCCTCGACGTGAAGGGCCGCAGTGCACAGACTCTGCTGCATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCCGTTCTCGTGAAACCGTGGGagg                       >  2:554535/1‑147 (MQ=255)

                                                                                                          cGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGCCGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGa               >  1:143391/1‑149 (MQ=255)

                                                                                                          cGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGa               >  1:76599/1‑149 (MQ=255)

                                                                                                           gTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGGCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGGAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCGTTCTGaa              >  1:336766/1‑149 (MQ=255)

                                                                                                           gTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGAGGCATTCTGaa              <  1:620916/149‑1 (MQ=255)

                                                                                                                gggCAGCATTGCACAGACTTTTTTGAATCGTTAAAAACAACCAGACGCTATGGCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGTGACGCATTCTGAAGATGc         <  2:451955/149‑1 (MQ=255)

                                                                                                                   ccGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTgg      <  1:483143/149‑1 (MQ=255)

                                                                                                                       aTTCCACAGACTCTGCTGAATCTGGAAAAACCCCCCAACAAATCCATGGAGCCTAACAGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTTGGACGCATTCTGAAGATGCTGGAAGa  <  1:211432/149‑1 (MQ=255)

GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGA  >  NC_000913_3_bme_pgi/3486430‑3486697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A